Abstract: An improved method for measuring the activity of a promoter sequence in a mammalian cell using a reporter gene is provided. The improvement involves using a reporter cassette containing a DNA sequence encoding a cytochrome P450 with a polyadenylation signal sequence as the reporter gene. Compositions containing the cytochrome P450 reporter cassette also are provided.
Type:
Grant
Filed:
August 22, 1996
Date of Patent:
March 10, 1998
Assignees:
Gentest Corporation, The United States of America as represented by the Secretary of the Department of Health and Human Services
Inventors:
Charles L. Chrespi, Bruce W. Penman, Frank J. Gonzalez, Harry V. Gelboin, Talia Sher
Abstract: The invention provides monoclonal antibodies and other binding agents to human cytochrome P450 1A2 having advantageous properties, including capacity substantially to inhibit enzyme activity of human cytochrome P450 1A2 and lack of specific binding to other human cytochromes P450. The binding agents of the invention are useful inter alia in methods for screening drugs for metabolism by cytochrome P450 1A2, and in methods of measuring p450 1A2 levels in individuals relative to p450 1A2 levels in a control population.
Type:
Grant
Filed:
April 8, 1999
Date of Patent:
January 1, 2002
Assignee:
The United States of America as represented by the Department
of Health and Human Services
Inventors:
Harry V. Gelboin, Frank J. Gonzalez, Tian J. Yang
Abstract: The invention provides monoclonal antibodies and other binding agents to human cytochrome P450 2A6 having advantageous properties, including capacity substantially to inhibit enzyme activity of human cytochrome P450 2A6 and lack of specific binding to other human cytochromes P450. The binding agents of the invention are useful inter alia in methods for screening drugs for metabolism by cytochrome P450 2A6, and in methods of measuring p450 2A6 levels in an individual relative to p450 2A6 levels in a control population.
Type:
Grant
Filed:
April 8, 1999
Date of Patent:
November 27, 2001
Assignee:
The United States of America as represented by the Department
of Health and Human Services
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