Abstract: The present invention relates to the use of a protein comprising an LOV domain for the photosensitive defunctionalization of a molecule and to a method for the photosensitive defunctionalization of a target molecule.
Type:
Application
Filed:
August 16, 2011
Publication date:
September 5, 2013
Applicant:
EVOCATAL GMBH
Inventors:
Karl Erich Jaeger, Thomas Drepper, Stephan Endres, Janko Potzkei
Abstract: The present invention provides variants of fluorescent proteins, which are improved with regard to their properties for use as reporter proteins and/or in analytics. In particular, variants of fluorescent proteins are provided, which fluoresce brighter, show improved quantum yield and/or have shifted excitation or emission spectra. The fluorescent proteins according to the invention comprise in their LOV domain besides the substitution of a cysteine with an amino acid that does not covalently bind FMN at least one further point mutation.
Type:
Application
Filed:
May 19, 2010
Publication date:
December 2, 2010
Applicant:
EVOCATAL GMBH
Inventors:
Thorsten Eggert, Sascha Hausmann, Michael Puls
Abstract: The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants (Arabidopsis) from which this enzyme or its gene is isolated is also described as non-cyanogenic. All HNL-containing plants described so far are cyanogenic plants and so it has until now been assumed that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly, it transpires that a polypeptide (AtHNL) of the invention is (R)-selective. The amino acid sequence gives a theoretical molecular weight of 29.2 kDa for the AtHNL subunit. The calculated molecular mass of the protein of approximately 30 kDa can be confirmed by SDS gel electrophoresis.