Abstract: Systems, and their methods of use, for sorting or separating magnetic particles are provided. A system having a magnetic module with features that mate with voids in a flow module exerts a magnetic field on magnetic particles to separate particles.

Abstract: Provided herein are devices useful in processing fat for fat grafting and for delivering fat tissue grafts to a patient. Also provided are devices and methods for fat grafting and for treatment of plantar fasciitis.

Abstract: A blood treatment system employing at least two pumps for performing a low blood flow treatment is operable for high blood flow treatments such as extracorporeal membrane oxygenation (ECMO) by providing a fluid circuit with parallel blood flow paths and recruiting multiple ones of the pumps for parallel blood flow.

Abstract: A bioprocessing system includes a first module configured for enriching and isolating a population of cells, a second module configured for activating, genetically modifying, and expanding the population of cells, and a third module configured for harvesting the expanded population of cells.

Abstract: A gas filter device for a medical device that provides a gas to a patient including first and second gas filter elements; a body defining a cavity receives the first and second gas filter elements in series and spaced apart from, each other; first and second end walls at opposites ends of the cavity; a first coupler extending from the first end wall; a second coupler extending from the second end wall, wherein the first coupler and the second coupler define a flow channel in communication with the cavity.

Abstract: A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, first and second containers selectively connected to the first outlet; and a magnet. The system also includes a controller configured to operate the spinning membrane to receive biological fluid and to direct the target cells to the first container, to pause to permit magnetic particles to be associated with the target cells in the first container, to operate the spinning membrane to receive the contents of the first container with the magnet applied to the target cells associated with the magnetic particles, to remove or deactivate the magnet, and to transfer the target cells to the second container.

Abstract: Disclosed herein are systems and methods capable of identifying, tracking, and sorting particles flowing in a channel, for example, a microfluidic channel having a fluid medium. The channel and the fluid medium can have a similar refractive index such that they appear translucent or transparent when illuminated by electromagnetic radiation. The particles can have a refractive index substantially different from that of the channel and the medium, such that the particles interfere with the electromagnetic radiation. A sensor can be disposed adjacent to the channel to record the electromagnetic radiation. The sensor can be used for identifying, tracking, and sorting the particles.

Abstract: A reagent selection support apparatus for supporting selection of a reagent used for cell measurement is provided. The apparatus includes a processing unit configured to acquire order information including a first measurement item and a second measurement item different from the first measurement item, and determine a combination of a first fluorescence reagent used to measure a first target molecule corresponding to the first measurement item and a second fluorescence reagent used to measure a second target molecule corresponding to the second measurement item, based on information on a property of the first target molecule and a property of a first fluorescent stain contained in the first fluorescent reagent, and information on a property the second target molecule and a property of a second fluorescent stain contained in the second fluorescent reagent; and an output unit configured to output the determined combination of the first fluorescence reagent and the second fluorescence reagent.

Abstract: Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.

Abstract: According to one embodiment of the present disclosure, a cell culture system includes: a cell culture container; a liquid storage part configured to store a liquid including a culture medium or a reagent to be supplied to the cell culture container; and a cell collection part configured to collect cells cultured in the cell culture container, wherein the cell culture container, the liquid storage part, and the cell collection part are connected by spatially closed-system lines at least during a period from feeding of the liquid to the cell culture container to removal of the cultured cells, and wherein the cell culture container is arranged in an incubator in a form of a multistage shelf including a liquid supply/discharge port.

Abstract: The invention relates to a device for separation of fluidal biological material components, particularly separation of components of the material collected by liposuction for subsequent transfer of the adipose tissue in an injection form. The device comprises a container with an outlet duct arranged in it, the outlet duct having its upper end adapted for collecting the material, and its lower end that is in fluid communication with the inner space of the container at its bottom. Further, the invention relates to a separating float for separation of the fluidal biological material components, the float being intended to be used in the device, wherein the float comprises at least one by-pass opening for passage of the fluidal biological material contained in the container from the area below the separating float into the area above it. The invention also relates to a kit including the device and the float.

Abstract: An assay plate assembly comprising a plurality of microfluidic modules arranged in a rectilinear matrix of rows and columns microfluidic channels. Each microfluidic module has an inlet well leading to a serpentine microfluidic channel that is set at a cant angle. The well is laterally offset from the detection area to avoid optical interference. The geometric center of each detection area is positioned according to ANSI/SLAS standards for well-centers. A drain from each microfluidic channel is located so that it does not interfere with any detection areas. An array of micro-posts are disposed within each microfluidic channel. The micro-posts extend perpendicularly from the top surface of the top plate toward the underside and are equally distributed throughout the entire detection area. The plate assembly provides reduced assay time and sample volume, and increased sensitivity and specificity in biological and chemical assays.

Abstract: The present invention relates to a device and a method for FIG. 2 treating fat cells taken from a patient and intended for a transplant. More specifically, it relates to a device and a method by suctioning which makes it possible to separate the viable fat cells from the residues and elements which are not transplantable.

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

Abstract: The present invention aims to provide a device for cryopreservation which enables easy and reliable cryopreservation of a cell or tissue. The device for cryopreservation of a cell or tissue of the present invention includes a deposition part on which a cell or tissue is to be deposited together with a preservation solution, wherein a surface of the deposition part includes a protrusion for holding a cell or tissue and a recess for storing a preservation solution.

Abstract: Disclosed are methods and devices for processing lipoaspirate that include mechanically-processing harvested lipoaspirate in a liposuction filter canister. In some embodiments, the devices are liposuction devices that include a lipoaspirate processing unit for mechanically-processing lipoaspirate. The mechanical processing reduces the average size of adipose tissue pieces in the lipoaspirate without substantially rupturing lipocytes therein.

Abstract: Incubators including an enclosure with an internal chamber configured to support a cell culture plate comprising a plurality of wells are disclosed. The enclosure includes a plurality of openings configured to allow access to the wells. The incubators include a sealing element configured to seal the plurality of openings in the enclosure. The sealing element comprises a plurality of openings corresponding to at least a subset of the plurality of openings in the enclosure. Access to the internal chamber can be provided by aligning the plurality of openings in the sealing element with the plurality of openings in the enclosure. Methods for using the incubators are also provided.

Abstract: The invention relates to a porous planar cell capture system for use in determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using such a cell capture system. The cell capture system contains a fluid permeable, planar membrane adopted to retain cells thereon, a fluid permeable support member that supports the membrane, and an optional register associated with the membrane.

Abstract: A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C1 in the biological sample from passing and allows a second cell C2 having a smaller diameter than that of the first cell C1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L1 mainly including the first cell C1 and a second liquid L2 mainly including the second cell C2. A measurement target cell filtered by the filter among other cells can be easily collected.

Abstract: An apparatus for passive sorting of microdroplets including a main flow channel, a flow stream of microdroplets in the main flow channel wherein the microdroplets have substantially the same diameter and wherein the flow stream of microdroplets includes first microdroplets having a first degree of stiffness and second microdroplets having a second degree of stiffness wherein the second degree of stiffness is different than the first degree of stiffness. A second flow channel is connected to the main flow channel for the second microdroplets having a second degree of stiffness. A separator separates the second microdroplets having a second degree of stiffness from the first microdroplets and directs the second microdroplets having a second degree of stiffness into the second flow channel.

Abstract: The invention relates to a method for separating off a liquid supernatant from cells, comprising the steps: providing a mixture of the cells with a liquid, charging a first filter housing with the mixture, wherein in the filter housing a filter having a pore size of between 4 ?m and 50 ?m is provided on a flat base surface pierced in a sieve manner and the walls of the filter housing are connected so as to seal with the flat base surface that is pierced in a sieve manner, applying a differential pressure of at least 0.5 bar on the mixture, as a result of which the liquid portion of the mixture is forced through the filter and a filter cake containing cells remains in the filter housing, and removal of the filtered liquid.

Abstract: An acoustophoresis device made up of modular components is disclosed. Several modules are disclosed herein, including ultrasonic transducer modules, input/output modules, collection well modules, and various connector modules. These permit different systems to be constructed that have appropriate fluid dynamics for separation of particles, such as biological cells, from a fluid.

Abstract: An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.

Abstract: A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed.

Abstract: A bismuth-containing concentration agent for microorganisms is provided. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.

Abstract: A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway.

Abstract: The present invention relates to an electromagnetic bioaccelerator for obtaining biomass by simulating environmental marine conditions, comprising at least the following elements: octagonal biomass converters (1), seawater reserve tanks (3), particle filters (4), UV light filters (5), feedback and mixture tanks (6), pressurization feed tanks (8), manometers (9), pressure controllers (10), buffer tanks (11), expansion tanks with a safety valve (12), heat exchangers (13), temperature control thermostats (14), recycled water feedback tanks (15), reinjection pumps (16), centrifuges for separating the biomass from the water (17), desuperheaters (18); control panels (25), recirculation pumps (26), densimeters (27), biomass mechanical extraction systems by means of centrifugation (32) and biomass accumulation tanks (33).

Abstract: A method and device is provided for collecting filterable material to perform assays on a filtration apparatus by applying negative-pressure to the inlet chamber of the filtration apparatus which is independent of negative or positive pressure applied to its fluid path. The negative-pressure applied to the inlet chamber serves to actively aspirate fluid and promote flow into the inlet chamber via the fluid pathway. The negative-pressure applied to the inlet chamber facilitates the use of either negative or positive-pressure based fluid-filtration approaches. Use of the invention improves the functionality and scope of applications that can be performed with enclosed filtration apparatuses.

Abstract: The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.

Abstract: The invention describes an appliance and a method, with the help of which specific bio-particles, but also dissolved bio-molecules can be recognized in and separated from fluids making use of suitable carriers and known immobilization methods. The appliance can be used both discontinuously and also for direct and continuous treatment of fluids. Fields of application of the invention are animals, bio-technology (including biological research) and medicinal diagnostics. Areas of application of the invention comprise, among others, therapy of humans, in particular direct treatment of blood.

Abstract: The invention relates to a filtration unit, comprising a membrane filter that can be disposed on a filter support of a bottom part and an attachment that can be placed on the bottom part, wherein the membrane filter has a reinforcing edge, and wherein the membrane filter can be clamped to a clamping part of a cover of a culture medium unit for removal purposes and introduced into the culture media unit. The invention further relates to a method for the microbiological analysis of liquid samples following a filtration process, wherein after removing an attachment a membrane filter is lifted off a filter support for pouring in the liquid sample and set down on a surface of a culture medium disposed in the bottom part of a culture medium unit, and the bottom part is covered by a cover. The cover is placed on the membrane resting on the filter support such that a clamping part present on the filter support clamps to a reinforcing edge of the membrane.

Abstract: Embodiments described herein relate to separating and/or concentrating target cells from a carrier fluid that may include other non-target cells. Embodiments include a cell separator with a flow surface having indentations formed thereon. The indentations are configured to capture target cells by physical and/or chemical interactions. The indentations may also include a layer of support molecules that assist in releasing captured cells for collection.

Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

Abstract: Hydrophobically modified polysaccharide compositions, articles incorporating the compositions, and methods for cell culture, automatic release of cells, and release of cell culture coating from a substrate or the cell culture articles incorporating the coating compositions, as defined herein.

Abstract: The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample.

Abstract: The invention concerns a pump cart for a biological liquid treatment installation, having a first lateral face (40), and a second lateral face (41) by which it is configured to be juxtaposed against a conveying network cart (1) of the installation and a front face (42) meeting the lateral faces (40, 41), and comprising at least one pump (414), a pump support (50) on which is mounted said pump (414), and a guide member for making said pump (414) movable in translation and on which said support (50) is mounted; said support (50) being movable in translation in a direction going from the first lateral face (40) towards the second lateral face (41); whereby said pump (414) is disposed at a predetermined location on said cart (2) depending on the type of treatment carried out.

Abstract: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as animal cells from fluid-cell mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of falling cells as the cells coalesce, agglomerate, and the weight of the agglomerated mass overcomes the drag and ultrasonic standing wave forces in the device.

Abstract: The invention concerns a device comprising: a base (2); a moveable or removable door (20), said device having a closed door position; and in the closed door position, a circuit (8) comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed on said front face (5) of said base (2) and a second shell (17) disposed in said door (20); said bag being clamped between said first shell (16) and said second shell (17) in a state in which conduits of said network for conveying liquid are formed between said films.

Abstract: A sample preparation apparatus comprising: a storage chamber that can store therein a liquid sample including an analysis target to be analyzed; a concentrated sample storage chamber that is provided to communicate with the storage chamber and that stores therein concentrated liquid having an analysis target having a higher concentration than that of the liquid sample; and an analysis target transportation section for transporting the analysis target included in the liquid sample stored in the storage chamber to the concentrated sample storage chamber. A cell analyzer is also disclosed.

Abstract: The invention concerns a device comprising a base (2) and a door (20), said device having a closed door position in which a circuit (8) of the device comprises a bag comprising two flexible films and connectors of the conveying network, and a press (9) comprising a first shell (16) disposed on a front face (5) of said base (2) and a second shell (17) disposed in said door (20); and a hinge system hinging said door (20) relative to said base (2), and disposed only on one side of said door (20) so as to form lateral clearances between said door (20) and said base (2) over the rest of a perimeter of said door (20).

Abstract: A method to recover and harvest nutrients from a liquid stream by incorporating them into microorganisms grown in a rotating photobioreactor. The method further includes optionally integrating the rotating photobioreactor with a composting or biogenic drying process.

Abstract: The invention provides a settling device comprising an acoustic wave generator and an inclined settling chamber. The angle ? between the acoustic wave direction and the inclined settling chamber is greater than 0 and less than 90°. The invention also provides a concentration method and a separation method using the device. The invention can be used to concentrate or separate particles such as inorganic particles, organic particles, and biological particles, for example, mammalian cells, bacteria, yeast, algae, and plant cells. The invention exhibits technical merits such as higher efficiency, cost-effectiveness, and large-scale production.

Abstract: The present invention relates to a method of sampling biological material on a reference surface, such as the surface of an agar medium, with the aid of a sampling tool, said method making it possible to steer the displacement of the sampling tool towards the reference surface, from a first position towards a second position, and/or of the reference surface towards the sampling tool, from a first position towards a second position, said second position being a contact position in which the sampling tool and the reference surface are in contact, said method comprising the following steps: a) illuminating the sampling tool in said first position in order to make the sampling tool visible and to project an image of the sampling tool onto the reference surface, b) acquiring an image of both the sampling tool and its image projected onto the reference surface, c) processing the image obtained in step b) in order to determine if, on the processed image, the gap between the sampling tool and its projected image is

Abstract: An assembly capable of capturing and purifying expressed biological products during or at the end of a bioreaction cycle is disclosed wherein a binding resin is kept separated from the contents of the bioreactor allowing capturing, harvesting and purification of biological products in a bioreactor; the invention additionally provides means of removing undesirable metabolic products as well as provides for efficient loading of chromatography columns.

Abstract: The present invention relates to a system for detecting microbial contamination of a liquid specimen comprising a device for concentrating micro-organisms from a liquid specimen, having (i) a hyperbaric chamber, (ii) a filter housing comprising a liquid-permeable bed of an adsorbent material and adapted for being fluidly connected to the hyperbaric chamber, and (iii) a manner for pressurizing the hyperbaric chamber. The system also comprises a kit for detection of micro-organisms adsorbed to the adsorbent material, wherein the kit is based on enzymatic detection using chromogenic and/or fluorescent substrate analogues.

Abstract: Systems and methods for processing biological material utilizing chitosan are provided herein. Methods may include the steps of reducing the pH of an amount of culture to about 5 to 7, incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material; increasing the pH of the culture to about 7 to 10, to flocculate biological material in the culture, concentrating the flocculates, reducing the pH of the concentrated flocculates to about 3 to 5, and separating the recovered chitosan solution from the concentrated biological material.

Abstract: A device and a method for isolating a target from a sample are provided. The target is bound to solid phase substrate to form a target bound solid phase substrate. The device includes a first plate having a first region for receiving at least a portion of the sample. A second plate is spaced from the first plate by a distance and has a first region for receiving a reagent. A force attracts the target bound solid phase substrate toward the first region of the second plate such that the target bound solid phase substrate in the portion of the sample are drawn through the air gap and into the reagent by the force.

Abstract: Methods are disclosed for isolation of a stem-cell derived differentiated cell, which method entails illuminating an incident light onto a plurality of stem-cell derived cells that comprise at least an undifferentiated cell and a differentiated cell possessing a noncentrosymmetric structure, wherein the differentiated cell generates second-harmonic light from the incident light; and isolating the differentiated cell identified by the second-harmonic light. Devices for carrying out the methods are also provided.

Abstract: The disclosure relates to an apparatus for segregating particles on the basis of their ability to flow through a stepped passageway. At least some of the particles are unable to pass through a narrower passageway bounded by a segregating step, resulting in segregation of the particles. The breadth of the leading edge of at least one step of the apparatus is significantly greater than the overall width of the passageway in which the step occurs, permitting high and rapid sample throughput. The apparatus and methods described herein can be used to segregate particles of a wide variety of types. By way of example, they can be used to segregate circulating tumor cells from a human blood sample.

Abstract: A method for sorting motile cells includes introducing an initial population of motile cells into an inlet port of a microfluidic channel, the initial population of motile cells having a first average motility; incubating the population of motile cells in the microfluidic channel; and collecting a sorted population of motile cells at an outlet port of the microfluidic channel. The sorted population of motile cells has a second average motility higher than the first average motility.