Abstract: The problem of detecting whether a urine sample is true human urine or a counterfeit urine product is solved by the use of reagent systems that detect two markers normally present in human urine. The markers acid phosphatase and alkaline phosphatase catalyze the substrates thymolphthalein monophosphate and p-nitrophenol phosphate, respectively. These substrates are formulated as spot tests on a dip stick or as reagents for use in automated chemical analyzers. The presence of the markers can be qualitatively detected by color-changes in the sample, formed by the pH-specific chromogens that result from catalysis of the substrates with the markers. The control reagent can further indicate whether a counterfeit urine product contains one or both of the chromogens.

Abstract: Described herein are kits for assessing the calcium oxalate titration test of an animal as well as methods for predicting the risk of calcium oxalate stone formation. Further described herein are methods and compositions for—inter alia—lowering the specific gravity of urine and lowering the calcium oxalate titration test in felines. In particular, diets and methods utilizing certain amounts and ratios of arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are disclosed herein.

Abstract: A building construction product comprising an ionochromic dye, wherein the building construction product has a first color when the product comprises water, wherein the first color is dependent on the presence of metal ions in solution in the product; and wherein the product has a second color when the product has solidified. Methods of monitoring setting and drying reactions of building construction products, including setting-type and drying-type joint compounds and plasters.

Abstract: A water hardness-measuring method and a fouling prevention method for hardness-measuring device include adding a colorant containing Eriochrome Black T and/or Calmagite and a sulfate ester-type anionic surfactant having a bonding unit derived from a sulfate ester including an alkyl group having 8 to 18 carbon atoms that may have one or more substituent in its chemical formula to a sample water.

Abstract: To provide to a reagent composition used in quantitative measurement of lithium in aqueous solutions such as biological specimens and environmental liquid samples by a simple colorimeter or ultraviolet-visible light spectrophotometer immediately, a reagent composition which permits to measure a concentration of lithium by visual observation, and a method and apparatus for measuring lithium ion by using the reagent. A reagent composition for measuring lithium comprising F28 tetraphenylporphyrin compound as chelating agent, including further a water-soluble organic solvent, a pH modifier and a stabilizer, and a method and apparatus for measuring lithium ion by using the reagent.

Abstract: A sensor including a fluorinated receptor can be used to identify an analyte through shift in 19F NMR resonance of the receptor when the receptor interacts with the analyte.

Abstract: Compositions and methods for increasing fluorescent signals generated by biomarkers are described. This serves to increase the accuracy of results when the biomarkers are used for the detection and diagnosis of physiological conditions, such as organ function and plasma volume.

Abstract: The present disclosure is directed to methods and systems for detecting a chemical substance. The methods and systems include chemically modifying a sample of a substance of interest through combination with a reagent to increase the volatility of the substance of interest. The systems and methods further include performing an analysis of the substance of interest.

Abstract: [Problem] To provide to a reagent composition used in quantitative measurement of lithium in aqueous solutions such as biological specimens and environmental liquid samples by a simple colorimeter or ultraviolet-visible light spectrophotometer immediately, a reagent composition which permits to measure a concentration of lithium by visual observation, and a method and apparatus for measuring lithium ion by using the reagent. [Solution] A reagent composition for measuring lithium comprising F28 tetraphenylporphyrin compound as chelating agent, including further a water-soluble organic solvent, a pH modifier and a stabilizer, and a method and apparatus for measuring lithium ion by using the reagent.

Abstract: Methods, computer program products, NMR assays and automated/semi-automated systems measure concentrations of ionized calcium and/or magnesium or other metabolites in clinical biosamples using NMR data obtained from an NMR spectrometer, such as a clinical NMR Analyzer.

Abstract: Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (Mc), a reporter molecule and two or more lipophilic groups (GL) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.

Abstract: The current invention provides compositions, which are useful as stationary phases for a variety of chromatographic applications, such as high performance liquid chromatography (HPLC) and solid-phase extraction (SPE). The compositions include a porous solid support (e.g., silica gels, silica monoliths or synthetic organic resins) having an exterior surface and pore openings defined by “interior walls”. To the solid support are covalently bound organic ion-exchange ligands (e.g., silyl ligands), which incorporate at least one ion-exchange group (e.g., ionic or ionizable group). The compositions further include micro-particles (e.g., latex particles) incorporating ion-exchange groups having a charge that is opposite to the charge found on the support. The micro-particles are bound to the exterior surface of the support (e.g., via electrostatic forces).

Abstract: Nanoporous polymorphic crystals of CaCO3 were used as sorbent and were applied in the dispersive micro-solid-phase extraction of selected polycyclic aromatic hydrocarbons as target analytes. After separation of the analytes on gas chromatography, they were successfully quantified with external calibration using flame ionization detection. Performance of the dispersive micro-solid-phase extraction was compared with a previously optimized solid-phase extraction technique.

Abstract: A safer, low-cost practical method of dissolving beryllium or a beryllium compound including beryllium oxide in a sample is disclosed. This method discloses use of acidic solutions under mild heating conditions to dissolve beryllium and its compounds. These solutions may then be used to quantitatively analyze for beryllium so as to be able to determine the amount of beryllium in the sample. These solutions may also be combined with fluorescent dye indicating solutions to determine beryllium by fluorescence. A preferred fluorescent dye indicating solution contains a dye (10-hydroxybenzo[h]quinoline-7-sulfonate) which specifically binds with beryllium under basic conditions.

Abstract: A system and method to generate a concentration gradient eluent flow are described. The concentration gradient eluent flow can include at least two different generants. A liquid can be pumped to an eluent generating device. A first controlling signal can be applied to a first eluent generator to generate a first generant. A second controlling signal can be applied to a second eluent generator to generate a second generant. Either the first and/or the second controlling signal can be varied as a function of time to generate the concentration gradient eluent flow.

Abstract: This invention relates to methods and apparatus for determination of ion concentrations, particularly in downhole water from hydrocarbon wells, aquifers etc. It is useful in a wide range of applications, including predicting the formation of scale and fingerprinting waters from different sources. More particularly, the invention relates to the use of ligands whose electronic configuration is altered by the binding of the scaling ions within a water sample. These alterations are detected electrochemically by applying varying potential to electrodes and measuring current flow as potential is varied, from which is derived the concentration of scaling ions in the fluid.

Abstract: The preparation of silver quantum clusters embedded in organic-templated-boehmite-nanoarchitecture (OTBN) and its use as a sensor for quantity of water flow measured by change of color in visible light upon flow of contaminated water have been provided. Silver quantum clusters-embedded OTBN are highly luminescent. Since the quantum clusters are embedded in the matrix, they are highly stable over a long period of time. The composition described here is utilized in the form of a device for ‘visible/ultraviolet light color change-based detection’ upon passage of water through a water purification device. Upon interaction with ions present in water, luminescent silver clusters undergo chemical transformation to Ag2S nanoparticles. The transformation is reflected in the form of visible color change (from pink to black) and luminescence quenching (from red emission to negligible luminescence).

Abstract: The present invention provides an electrochemical sensor having a solid electrically conductive substrate and a compound immobilized thereon which has binding affinity for an analyte species to be detected and also displays electrochemical behavior which is modified upon binding of that analyte species, so that binding of an analyte species can be detected by measuring electrochemical properties, such as by voltammetry. Desirably the immobilized compound contains both a first moiety with binding affinity for the analyte and a separate second moiety which is a redox system whose electrochemical oxidation/reduction properties become modified when the first moiety binds the analyte. The analyte binding moiety may be a diol or polyol and preferably is a sugar. The electrochemical sensor finds use in methods of analyzing reservoir and process fluids, particularly at a subterranean location. The electrochemical sensor may be a component of a downhole tool, adapted for deployment downhole.

Abstract: [Problem] To provide to a reagent composition used in quantitative measurement of lithium in aqueous solutions such as biological specimens and environmental liquid samples by a simple colorimeter or ultraviolet-visible light spectrophotometer immediately, a reagent composition which permits to measure a concentration of lithium by visual observation, and a method and apparatus for measuring lithium ion by using the reagent. [Solution] A reagent composition for measuring lithium comprising F28 tetraphenylporphyrin compound as chelating agent, including further a water-soluble organic solvent, a pH modifier and a stabilizer, and a method and apparatus for measuring lithium ion by using the reagent.

Abstract: A package comprising a body comprising a first compartment containing a first substance and a second compartment containing a second substance. The package includes a first state in which the first compartment is isolated from the second compartment such that the first substance is separated from the second substance, and a second state in which the first compartment communicates with the second compartment such that the first substance and the second substance combine.

Abstract: Reagents and methods for determination of calcium within a sample. Specific embodiments include a reagent for determination of calcium comprising a mono-nitro substituted BAPTA-type chelator (BAPTA=1,2-bis(2-aminophenoxy)ethane-N,N,N?,N?-tetraacetic acid). Methods for accurate determination of calcium in a sample, such as a blood, whole blood, plasma or serum or any other aqueous liquid sample including cerebrospinal fluid, lymph, salivary juice or urine. Embodiments are also useful for clinical diagnoses.

Abstract: Provided are automated methods for measuring soluble magnesium concentration in water using fluorescence. The methods employ the use of a pH-buffered liquid and a magnesium coordinating fluorescing reagent. In certain embodiments, the methods may further employ measuring total hardness concentration of the water by displacing any soluble calcium with soluble magnesium and then re-measuring the soluble magnesium concentration. Optionally, the soluble calcium concentration can be determined by subtracting the measured soluble magnesium concentration from the measured total hardness concentration.

Abstract: A low-cost practical method of determining beryllium or a beryllium compound thereof in a sample is disclosed by measuring fluorescence. This method discloses optical filters for use with low cost fluorometers which may be used to quantitatively determine the presence of beryllium. This method may be extended to estimate particle size distribution for particles comprising Beryllium. A method is also disclosed to store solutions for extended period of time so that these materials are stable during transportation and in the labs.

Abstract: A method of screening for increased risk of fatal prostate cancer in a subject comprises providing a blood sample collected from the subject, and then detecting the presence or absence of an increased level of serum calcium in the sample. An increased level of serum calcium indicates the subject is at increased risk of fatal prostate cancer.

Abstract: Coelenterazine analogs with different luminescence properties from conventional ones and coelenteramide analogs with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogs modified at the 8-position of coelenterazine and coelenteramide analogs modified at the 2- or 3-position of coelenteramide.

Abstract: The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc.

Abstract: An aquatic environment monitoring system and method that includes correction for adverse conditions in the monitoring system involving the development of confidence levels for certain conditions in the monitoring system using stored information related to the aquatic environment and/or the monitoring system. Corrections to adverse conditions may be made by the environment monitoring system automatically by the monitoring system and manually via communications to a user of the system.

Abstract: A system and method for determining a remaining processing capacity of a scrubber having a flow path and a processing material disposed along the flow path. A device may comprise a plurality of optical sensors disposed within the processing material and arranged along the flow path, a light source, and a processor for determining the capacity according to signals received from the optical sensor. The device may be used to illuminate processing material adjacent to each optical sensor using the light source, measure a light value reflected by the processing material at each optical sensor, and determine the remaining processing capacity of the scrubber, using the processor, based on the measured light value. Devices may comprise a memory, such as a non-volatile memory to allow multiple uses of a scrubber without reloading with fresh processing material.

Abstract: A method for determining the content of calcium element in an ore is provided. It includes: decomposing the ore with hydrochloric acid and nitric acid under heating condition, adding perchloric acid, cooling, adding a small amount of water and boiling the solution to dissolve the salts, then cooling, diluting to the constant volume, filtering into a dry beaker with dry filter-paper, masking interfering ions with triethanolamine, adjusting the pH value of the solution with KOH, using calcein-thymolphthalein as indicator, and determinating the content of calcium with EDTA titrimetry.

Abstract: A dry test strip for measuring calcium is comprising a support, a reagent layer provided on the support, and a reagent holding layer provided on the reagent layer, and containing, as reagents, o-cresolphthalene complexone, a magnesium selective masking agent, and a pH buffer for adjusting the pH of the environment for reaction of the o-cresolphthalene complexone with calcium to 10.0-11.0, wherein such reagents are present in either the reagent layer or the reagent holding layer or the both layers.

Abstract: A safer, low-cost practical method of dissolving beryllium or a beryllium compound including beryllium oxide in a sample is disclosed. This method discloses use of acidic solutions under mild heating conditions to dissolve beryllium and its compounds. These solutions may then be used to quantitatively analyze for beryllium so as to be able to determine the amount of beryllium in the sample. These solutions may also be combined with fluorescent dye indicating solutions to determine beryllium by fluorescence. A preferred fluorescent dye indicating solution contains a dye (10-hydroxybenzo[h]quinoline-7-sulfonate) which specifically binds with beryllium under basic conditions.

Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).

Abstract: In order to provide a reagent kit for detecting LA which can clearly separate a lupus anticoagulant (LA)-positive specimen group from an LA-negative specimen group, it is configured that the reagent kit for detecting LA contains a first clotting time-measuring reagent and a second clotting time-measuring reagent and at least one of the first clotting time-measuring reagent and the second clotting time-measuring reagent contains alkali metal salt. The presence or absence of LA can be determined using the kit.

Abstract: Methods and devices are disclosed providing techniques for measuring the amount of biodiesel in a fuel sample. The methods may be used in the field without the use of laboratory equipment. The biodiesel in the sample is converted to the corresponding free acid which can be isolated and quantified to provide information regarding the amount of biodiesel in the original sample.

Abstract: The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention.

Abstract: The method of the present disclosure determines whether a target DNA forms a G-quadruplex using a phenomenon in which thioflavin T generates a strong fluorescence when reacted with the G-quadruplex in the presence of potassium ions.

Abstract: A compound represented by the formula (I) (one of substituents represented by R1 is a trapping group for an object substance for measurement; R2 and R3 represent hydrogen, alkyl, or halogen; R4 and R5 represent alkyl or aryl; R6 and R7 represent hydrogen, alkyl, or halogen; R8 represents hydrogen, alkylcarbonyl, or alkylcarbonyloxymethyl, and X represents silicon, germanium, or tin, which can be used as a fluorescent probe that enables red color bioimaging using intramolecular photoinduced electron transfer.

Abstract: Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide.

Abstract: There has been a need for coelenterazine analogs that exhibit luminescence properties different from those of known coelenterazine analogs. The present invention provides the compound represented by general formula (1).

Abstract: An aquatic environment monitoring system that includes chemical indicators that are dyes immobilized in an immobilizing medium, such as a hydrogel, and supported on a holder such that an optical reader may be used to illuminate the indicator dyes. The chemical indicator dyes include chemical indicator dyes that are sensitive for detecting calcium, magnesium, and carbon dioxide present in the aquatic environment.

Abstract: The present invention relates to ?-conjugated fluoroionophores, methods for their preparation, and their use and a method for determining an alkali ion. Fluoroionophoric compounds of the general formula I are described Ionophore-?-Linker-Fluorophore??(I) wherein the Ionophore is an anilino containing crown ether or cryptand with one or more anilino donor moieties as electron donors, forming a stable complex with an alkali metal ion the ?-Linker is an aromatic or heteroaromatic conjugative linking moiety, and the Fluorophore is an electron acceptor moiety. Variation of the ionophoric unit offers a broad spectrum of detectable K+ and Na+-concentrations, ranging from high concentration around 800 mM down to very low concentrations around 3 mM.

Abstract: Systems and methods are provided for predicting the likelihood of acute kidney injury. An input interface is configured to receive a plurality of features derived from the results of a post-surgical metabolic blood panel and either a pre-surgical metabolic blood panel or a perisurgical metabolic blood panel. A predictive model is configured to calculate a parameter representing a likelihood of acute kidney injury from the plurality of features. A user interface is configured to provide the calculated parameter to a user in a human comprehensible form.

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

Abstract: An automatic method for identifying biological samples that are collected using the wrong blood preservative for subsequent analytical testing. The method also provides for identification and/or suppression of certain analytical test results that are substantially or partly adversely affected. The invention is particularly suited for use in point-of-care medical diagnostic testing.

Abstract: The invention is directed to a method of identifying a patient undergoing periodic hemodialysis treatments at increased risk for death that includes determining at least one of the patient's clinical or biochemical parameters, consisting of serum bicarbonate concentration level, serum potassium concentration level, serum calcium concentration level, hemoglobin concentration level, serum phosphorus concentration level, neutrophil to lymphocyte ratio, equilibrated normalized protein catabolic rate (enPCR), equilibrated fractional clearance of total body water by dialysis and residual kidney function (eKdrt/V), EPO resistance index, transferrin saturation index, serum ferritin concentration level, serum creatinine concentration level, platelet count, Aspartat-Aminotransferase level, and Alanin-Aminotransferase level at periodic hemodialysis treatments, and identifying a patient as having an increased risk for death if the patient has a significant change in the rate of change of at least one of the patient's cli

Abstract: The present invention provides an apparatus and rapid methods for extracting strontium ions from urine to provide a concentrated and purified strontium-90 extract suitable for scintillation measurements. The methods remove organic compounds, pigments, and alkali metal ions that can interfere with quantitative determination of strontium-90 in urine.

Abstract: The present technology provides a cell based assay for identifying compounds that modulate store-operated ionic calcium levels using itpr mutant cell lines, such as itpr-ku cells, which have abnormal ionic calcium levels.

Abstract: A method of determining beryllium or a beryllium compound thereof in a sample is disclosed by measuring fluorescence. This method discloses improved sample preparation methods, particularly for refractory beryllium materials. The method also discloses methods to improve the detection limit of beryllium including use of optical filters with specific characteristics for selecting the emission wavelengths of the fluorescence signal.

Abstract: Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, ? carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or GBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.

Abstract: A system to selectively deliver relatively small analyte molecules of interest to a SERS-active nanoparticle surface while excluding dozens to hundreds of other species in the environment. In particular, the present invention provides a permselective film that renders the particles of interest as viable small molecule optically addressable sensors.