Patents Represented by Attorney Allan M. Kiang
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Patent number: 7601491Abstract: The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.Type: GrantFiled: February 6, 2003Date of Patent: October 13, 2009Assignee: Becton, Dickinson and CompanyInventors: Matthew P. Collis, Donald W. Copertino, Karen Eckert, Thomas L. Fort
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Patent number: 7521185Abstract: Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the replicase gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARS-CoV infection.Type: GrantFiled: September 13, 2004Date of Patent: April 21, 2009Assignee: Becton, Dickinson and CompanyInventors: Jianrong Lou, James A. Price, Jr., Daretta A. Yursis, David M. Wolfe, Lisa M. Keller, Tobin J. Hellyer
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Patent number: 7229800Abstract: The present invention relates to nucleic acid amplification assays for the detection of nucleic acid sequences of Neisseria gonorrhoeae. The present invention provides oligonucleotides that are complementary or that anneal to nucleic acid sequences of Neisseria gonorrhoeae. The present invention also provides internal amplification controls (IACs) that can be used in nucleic acid amplification reactions.Type: GrantFiled: April 16, 2004Date of Patent: June 12, 2007Assignee: Becton, Dickinson and CompanyInventor: James M. Harris
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Patent number: 7223536Abstract: The present invention provides methods for detecting and identifying sequence variations in a nucleic acid sequence of interest using a detector primer. It has been found that the reduced efficiency of primer extension by DNA polymerases when the 3? end of a primer does not hybridize perfectly with the target can be adapted for use as a means for distinguishing or identifying the nucleotide in the target which is at the site where the diagnostic mismatch between the detector primer and the target occurs. The detector primer hybridizes to the sequence of interest and is extended with polymerase. The efficiency of detector primer extension is detected as an indication of the presence and/or identity of the sequence variation in the target. The inventive methods make use of nucleotide mismatches at or near the 3? end of the detector primer to discriminate between the nucleotide sequence of interest and a second nucleotide sequence which may occur at that same site in the target.Type: GrantFiled: February 7, 2001Date of Patent: May 29, 2007Assignee: Becton, Dickinson and CompanyInventors: David J. Wright, Maria A. Milla, James G. Nadeau, G. Terrance Walker
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Patent number: 7029840Abstract: The present invention provides a method for preserving cells and nucleic acids in a sample by exposing the sample to a composition that causes an inhibitory affect on proteolytic agents and/or nucleic acid degradative agents in the sample. Such compositions that are useful in the method of the present invention include chelating agents such as sodium citrate, sodium borate, sodium fluoride and EDTA, that will bind trace metals necessary for proteolytic activity of proteolytic agents and/or nuclease activity of nuclease agents in a sample.Type: GrantFiled: August 20, 2002Date of Patent: April 18, 2006Assignee: Becton, Dickinson and CompanyInventor: Ray A. McMillian
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Patent number: 6939696Abstract: The present invention relates to a method for disrupting cells by subjecting the cells to ultrasonic energy in the absence of beads. The present invention also relates to the enhancement of cell disruption methods using ultrasonic energy by reducing the surface tension of the liquid in which the cells are located.Type: GrantFiled: August 3, 1998Date of Patent: September 6, 2005Assignee: Becton, Dickinson and CompanyInventors: Oscar J. Llorin, Matthew P. Collis, Michael C. Little, James M. Harris
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Patent number: 6916608Abstract: The present invention relates to a method and composition for stabilizing clinical specimens (i.e., cells in biological samples) for transport and subsequent testing for diagnosis. The composition is specifically capable of maintaining nucleic acid in the cells intact for hybridization with oligonucleotide capture and detector probes.Type: GrantFiled: September 10, 1999Date of Patent: July 12, 2005Assignee: Becton, Dickinson and CompanyInventors: Dolores M. Berger, Daretta A. Yursis, William A. Nussbaumer, Anne B. Brown
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Patent number: 6770752Abstract: Primers and probes derived from the HIV-1 pol gene which facilitate detection and/or quantification of all presently known genotypes of HIV-1 (A-I and O). Disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of HIV nucleic acids.Type: GrantFiled: January 9, 2001Date of Patent: August 3, 2004Assignee: Becton, Dickinson and CompanyInventors: Tobin J. Hellyer, Qimin You, James M. Harris
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Patent number: 6743582Abstract: The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.Type: GrantFiled: June 28, 2001Date of Patent: June 1, 2004Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, Tobin J. Hellyer
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Patent number: 6682889Abstract: Amplification primers and methods for specific amplification and detection of a rnpB gene sequence are disclosed. The primer-target binding sequences are useful for amplification and detection of organisms of the Chlamydiaceae family in a variety of amplification and detection reactions.Type: GrantFiled: November 8, 2000Date of Patent: January 27, 2004Assignee: Becton, Dickinson and CompanyInventors: Sha-Sha Wang, David Wolfe
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Patent number: 6672458Abstract: A system and method for manipulating magnetically responsive particles in a solution to separate nucleic acid molecules from cell components in a cell solution. The system and method employ a device capable of receiving a plurality of tubes, each of which contain respective sample and magnetically responsive particles. The device includes heating and cooling devices to facilitate a lysing step to release the nucleic acid molecules from the cells in the cell solution. The device further includes moveable magnets which can be moved proximate to and away from the tube to hold the magnetically responsive particles to which the nucleic acid molecules become bound, so that the molecule-bound particles can be separated from the remainder of the solution, and washed as appropriate. The system also employs an electromagnet which is capable of demagnetizing the particles to allow the particles to freely mix with solution, such as elution solutions which are used to unbind the molecules from the particles.Type: GrantFiled: May 17, 2001Date of Patent: January 6, 2004Assignee: Becton, Dickinson and CompanyInventors: Timothy Roy Hansen, Bradley Scott Thomas, John Joseph Bianco, Matthew P. Collis
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Patent number: 6656680Abstract: The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.Type: GrantFiled: June 28, 2001Date of Patent: December 2, 2003Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, Tobin J. Hellyer
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Patent number: 6597450Abstract: An apparatus and method employing a plurality of light emitting devices which each can get light through a respective optical fiber toward a respective sample of a plurality of samples in a time-staggered manner. Light is generated in each of the samples at different times consistent with the times at which light is irradiated onto the sample. A single detector is used to detect the lights emitted from the plurality of samples at these different times. A plurality of bifurcated optical cable are coupled to the light emitting devices and single light detector, and the integrated end of each bifurcated cable acts as the light emitting port and light detecting port. Multiple targets can be detected from each of the plurality of samples in the same manner by providing an apparatus and method employing a different plurality of light emitting devices and single detector for each target to be detected.Type: GrantFiled: January 14, 2000Date of Patent: July 22, 2003Assignee: Becton, Dickinson and CompanyInventors: Jeffrey P. Andrews, Christian V. O'Keefe, Brian G. Scrivens, Willard C. Pope, Timothy Hansen, Frank L. Failing
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Patent number: 6586584Abstract: Primers and probes derived from the 5′ untranslated region of the HCV genome which facilitate detection and/or quantification of all presently known genotypes of HCV. Disclosed sequences may be used in a variety of primer and probe constructs for amplification and/or detection of HCV nucleic acids.Type: GrantFiled: January 29, 2001Date of Patent: July 1, 2003Assignee: Becton, Dickinson and CompanyInventors: Ray A. McMillian, Tobin J. Hellyer
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Patent number: 6583279Abstract: Primers and probes derived from the HBV DNA polymerase gene which facilitate detection and/or quantification of all presently known genotypes of HBV. Disclosed sequences may be used in a variety of primer and probe constructs for detection of HBV nucleic acids.Type: GrantFiled: January 26, 2001Date of Patent: June 24, 2003Assignee: Becton, Dickinson and CompanyInventors: Dolores M. Berger, William A. Nussbaumer, Thomas L. Fort, Tobin J. Hellyer
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Patent number: 6342655Abstract: CP genes of CMV strains V27, V33, V34, and A35 (CMV-V27, CMV-V33, CMV-V34, and CMV-A35 respectively) are provided.Type: GrantFiled: July 14, 2000Date of Patent: January 29, 2002Assignee: Seminis Vegetable Seeds, Inc.Inventors: Maury L. Boeshore, J. Russell McMaster, David M. Tricoli, John F. Reynolds, Kim J. Carney
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Patent number: RE39885Abstract: Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.Type: GrantFiled: May 20, 1998Date of Patent: October 16, 2007Assignee: Becton, Dickinson and CompanyInventors: James G. Nadeau, George T. Walker