Abstract: Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.
Type:
Grant
Filed:
January 5, 2006
Date of Patent:
July 1, 2008
Assignee:
Schering Corporation
Inventors:
Susan V. Cannon-Carlson, Collette Cutler, Gary J. Vellekamp, Marcio Voloch