Patents Represented by Attorney Danisco US Inc.
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Patent number: 7884192Abstract: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.Type: GrantFiled: June 1, 2007Date of Patent: February 8, 2011Assignee: Danisco US Inc.Inventors: Sierd Bron, Jan D. H. Jongbloed, Joerg Muller, Jan M. Van Dijl
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Patent number: 7858762Abstract: An evolvable production strain of B. subtilis exhibiting continuous or high level expression during protein evolution is described. An evolved Bacillus subtilis pstS promoter facilitates screening and production of secreted proteins.Type: GrantFiled: July 26, 2002Date of Patent: December 28, 2010Assignee: Genencor International, Inc.Inventors: Katherine D Collier, Edwin Lee, Nicholas Leiva, Thomas B Morrison, Volker Schellenberger, Olga V Selifonova, Adam C Kean
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Patent number: 7803591Abstract: Described are multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate. The complexes having a dehydrogenase subunit and a cytochrome C subunit. Also described are polynucleotides coding for the multimeric complexes and methods of use thereof.Type: GrantFiled: October 16, 2007Date of Patent: September 28, 2010Assignee: Genencor International, Inc.Inventors: Robert M. Caldwell, M. Harunur Rashid, Fernando Valle
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Patent number: 7723079Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.Type: GrantFiled: July 21, 2008Date of Patent: May 25, 2010Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward, III
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Patent number: 7629451Abstract: The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.Type: GrantFiled: July 2, 2008Date of Patent: December 8, 2009Assignee: Genencor International, Inc.Inventors: Kathleen A. Clarkson, Nigel Dunn-Coleman, Suzanne E. Lantz, Craig E. Pilgrim, Piet Van Solingen, Michael Ward
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Patent number: 7626004Abstract: The present invention relates to a method of purifying a protein of interest from its fusion analog, comprising obtaining a protein solution comprising a protein of interest and its fusion analog; adjusting the pH and/or ionic strength of the protein solution with an appropriate buffer for use with a Hydrophobic Charge Induction Chromatography (HCIC) resin, contacting the protein solution and HCIC resin column to allow binding of the protein of interest and its fusion analog, washing the resin and eluting the protein of interest from the resin by a pH gradient; wherein the protein of interest is substantially free of its fusion analog.Type: GrantFiled: March 13, 2007Date of Patent: December 1, 2009Assignee: Genencor International, Inc.Inventors: Robin Fong, Meng H. Heng
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Patent number: 7604974Abstract: The present invention provides a novel cellulase nucleic acid sequence, designated 029cel, and the corresponding 029cel amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding 029cel, recombinant 029cel proteins and methods for producing the same.Type: GrantFiled: April 28, 2004Date of Patent: October 20, 2009Assignee: Genencor International, Inc.Inventors: Brian E. Jones, William D. Grant, Shaun Heaphy, Susan Grant
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Patent number: 7592420Abstract: Described herein are protease inhibitors, variants thereof and methods for their production.Type: GrantFiled: August 30, 2007Date of Patent: September 22, 2009Assignee: Genencor International, Inc.Inventors: Hans De Nobel, David A. Estell, Wei Liu, Scott D. Power, Brian Schmidt, Huaming Wang
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Patent number: 7592157Abstract: Described herein are protease inhibitors, variants thereof and methods for their production.Type: GrantFiled: August 30, 2007Date of Patent: September 22, 2009Assignee: Genencor International, Inc.Inventors: Hans De Nobel, David A. Estell, Wei Liu, Scott D. Power, Brian Schmidt, Huaming Wang
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Patent number: 7563609Abstract: Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described.Type: GrantFiled: March 18, 2008Date of Patent: July 21, 2009Assignee: Genencor International, Inc.Inventors: Mark Donnelly, William H. Eschenfeldt, Jonathan Trent
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Patent number: 7563607Abstract: The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.Type: GrantFiled: December 21, 2005Date of Patent: July 21, 2009Assignee: Genencor International, Inc.Inventors: Gang Duan, Nigel Dunn-Coleman, Oreste Lantero, Craig E. Pilgrim, Jayarama K. Shetty
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Patent number: 7544490Abstract: The present invention relates to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms having exogenous nucleic acid sequences introduced therein and methods for producing proteins in such host cells, such as members of the genus Bacillus. More specifically, the present invention relates to the expression, production and secretion of a polypeptide of interest and to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention provides for the enhanced expression of a selected polypeptide by a microorganism.Type: GrantFiled: March 19, 2007Date of Patent: June 9, 2009Assignee: Genencor International, Inc.Inventors: Eugenio Ferrari, Anita Van Kimmenade
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Patent number: 7544488Abstract: The present invention relates to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms having exogenous nucleic acid sequences introduced therein and methods for producing proteins in such host cells, such as members of the genus Bacillus. More specifically, the present invention relates to the expression, production and secretion of a polypeptide of interest and to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention provides for the enhanced expression of a selected polypeptide by a microorganism.Type: GrantFiled: March 19, 2007Date of Patent: June 9, 2009Assignee: Genencor International, Inc.Inventors: Eugenio Ferrari, Anita Van Kimmenade
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Patent number: 7544489Abstract: The present invention relates to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms having exogenous nucleic acid sequences introduced therein and methods for producing proteins in such host cells, such as members of the genus Bacillus. More specifically, the present invention relates to the expression, production and secretion of a polypeptide of interest and to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention provides for the enhanced expression of a selected polypeptide by a microorganism.Type: GrantFiled: March 19, 2007Date of Patent: June 9, 2009Assignee: Genencor International, Inc.Inventors: Eugenio Ferrari, Anita Van Kimmenade
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Patent number: 7524816Abstract: The present invention provides peptides and supported peptides for treating proliferative diseases. In particularly preferred embodiments, the present invention provides peptides and supported peptides for treating diseases of the skin, such as rosacea. In some particularly preferred embodiments, the supported peptides of the present invention are anti-VEGF peptides. In alternative particularly preferred embodiments, the anti-VEGF peptides are expressed on a scaffold protein. In some most preferred embodiments, the scaffold proteins is BBI.Type: GrantFiled: November 8, 2004Date of Patent: April 28, 2009Assignee: Genencor International, Inc.Inventors: Anthony G. Day, David A. Estell, Christopher J. Murray, Scott D. Power
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Patent number: 7510831Abstract: A novel DNA is provided which encodes an enzyme having phytase activity isolated from Trichoderma. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.Type: GrantFiled: October 9, 2002Date of Patent: March 31, 2009Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward
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Patent number: 7498159Abstract: The present invention relates to the co-expression and production of a heterologous alpha amylase and an endogenous glucoamylase in an Aspergillus strain and enzyme compositions including the same.Type: GrantFiled: December 3, 2007Date of Patent: March 3, 2009Assignee: Genencor International, Inc.Inventors: Toby M. Baldwin, Kathleen A. Clarkson, Nigel Dunn-Coleman, Suzanne E. Lantz
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Patent number: 7494685Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.Type: GrantFiled: August 28, 2006Date of Patent: February 24, 2009Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward
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Patent number: 7407791Abstract: Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.Type: GrantFiled: August 6, 2003Date of Patent: August 5, 2008Assignee: Genencor International, Inc.Inventor: Maria R. Diaz-Torres