Abstract: Novel substantially pure periplasmic 3':5'-cyclic nucleotide phosphodiesterases are provided which are obtainable from gram negative bacterium capable of growing on restricted media containing cAMP or cGMP as a sole carbon source. Also provided is the isolated DNA coding for such enzymes and related methods of producing the same.

Abstract: The present invention relates to a monclonal antibody capable of suppressing the motility of cancer cells, a polypeptide recognizable by said anti-cancer antibody and its fragment peptides which is capable of suppressing the motility of cancer cells. The present invention also relates to a production and a use for preventing the matastasis of cancer thereof.

Abstract: A clip for earrings comprising: a movable spring action clip arm and a stationery body, said body having a attachment member at one end thereof and connection means at the other end thereof, one end of said clip arm having means for receiving said body connection means so as to pivotally attach said clip arm to said body for relative spring urged movment towards and away from said clip, said attachment member having a first and second side and an opening extending through said sides, said first side being adapted to receive a decorative element, said second side having a metallic band positioned above said opening and extending from the surface in an arc shape and adapted to receive an earwire.

Abstract: Provided are monoclonal antibodies, fragments, and derivatives thereof reactive with an epitope of the T cell receptor alpha chain variable region, V.alpha.12.1, on human T lymphocytes. The monoclonal antibodies are reactive with approximately 2% of CD4.sup.+ T lymphocytes and with approximately 5% of CD8.sup.+ T lymphocytes in peripheral blood cells in normal individuals and define a subset of individuals afflicted with an autoimmune disease, especially rheumatoid arthritis, that exhibit increased expression of the V.alpha.12.1 gene on CD8.sup.+ peripheral blood T lymphocytes when compared to normal individuals. Methods for treating individuals afflicted with such an autoimmune disease using V.alpha.12.1 specific reagents are described herein.

Abstract: The present invention provides recombinant DNA viral vectors which co-express lentivirus genes encoding structural and enzymatic polypeptides capable of assembling into defective nonself-propagating viral particles. The viral DNA vectors as well as the viral particles can be used as immunogens and for targeted delivery of heterologous gene products and genes.

Abstract: Recombinant viral vectors which coexpress heterologous polypeptides capable of assembling into defective non-self-propagating lentiviral particles are disclosed. The viral vectors as well as the viral particles can be used as immunogens and for targeted delivery of heterologous gene products and drugs.

Abstract: A modified Salmonella, wherein the wild type pag gene such as pagC has been replaced by a gene encoding a truncated pag gene such as pagC gene fused to a heterologous DNA segment, is disclosed. This modification significantly attenuates the virulence of the Salmonella. In addition, immune reaction to the portion of the fusion protein encoded by the heterologous gene can be generated. Uses of the vector, including its use in a drug screen are also disclosed.

Abstract: The present invention relates to a recombinant carcinoembryonic antigen (CEA)/vaccinia virus or other viral vector which expresses CEA on the surface of infected cells and which elicits an immune response in vivo directed against CEA or cells expressing CEA and a pharmaceutical composition containing same.

Abstract: O-substituted fumagillol derivatives and its salts have an angiogenesis inhibiting activity and are useful for prophylaxis and treatment of diseases induced by abnormally stimulated neovascularization.

Abstract: The ability of viruses to undergo recombination within tandemly arranged homologous sequences can be utilized to generate chimeric genes and proteins. Tandemly arranged homologous sequences will rapidly degenerate in a random fashion to yield a single copy comprised of portions of both original sequences. Therefore, a recombinant virus which contains two related but non-identical genes in tandem array yields a population of recombinant viruses which contain a spectrum of hybrid sequences derived from recombination between the original genes. The viruses, therefore, contain hybrid DNA sequences that encode proteins with new epitopes or different combinations of epitopes. Vaccines are derived which may afford protection against a broad spectrum of antigen types.

Abstract: This invention relates to a DNA fragment comprising a base sequence encoding a non-A non-B hepatitis-specific antigen polypeptide, said base sequence being obtained using genetic engineering techniques from non-A non-B hepatitis virus RNA which is isolated directly from blood plasma from non-A non-B hepatitis patients, to an expression vector and a transformant for use in the expression of the DNA fragment, to a single strand DNA sequence for PCR primer, and to use of said polypeptide and said single strand DNA sequence in the detection of the non-A non-B hepatitis virus. The recombinant polypeptide and the single strand DNA sequence for PCR primer make it possible to detect the non-A non-B hepatitis virus with extremely high accuracy.

Abstract: We have discovered that using non-integrative viral vectors having low replicative efficiency for insertion of a gene into a cell such as a lymphocyte or a tumor cell is a preferred system for transforming such cells for use in somatic cell therapy or gene therapy. These vectors are preferably cytoplasmic viral vectors, as opposed to nuclear viral vectors. Preferred cytoplasmic vectors include DNA viruses such as pox viruses and iridoviruses and RNA viruses such as picornavirus, calicivirus and togavirus. More preferably the virus used will not be capable of sustained replication in the target cell. For example, a preferred pox virus for human cells will be an avipox, or suipox in contrast to an orthopox virus such as vaccinia.

Abstract: This invention relates to a DNA fragment comprising a base sequence encoding a non-A non-B hepatitis-specific antigen polypeptide, said base sequence being obtained using genetic engineering techniques from non-A non-B hepatitis virus RNA which is isolated directly from blood plasma from non-A non-B hepatitis patients, to an expression vector and a transformant for use in the expression of the DNA fragment, to a single strand DNA sequence for PCR primer, and to use of said polypeptide and said single strand DNA sequence in the detection of the non-A non-B hepatitis virus. The recombinant polypeptide and the single strand DNA sequence for PCR primer make it possible to detect the non-A non-B hepatitis virus with extremely high accuracy.

Abstract: The present invention provides a cathepsin L inhibitor containing a compound of the formula: ##STR1## wherein R.sup.1 is a hydrogen atom or an arylalkyl, heterocyclic-alkyl or lower alkyl group which may be substituted; R.sup.2 and R.sup.3 independently are a hydrogen atom or a hydrocarbon residue which may be substituted; R.sup.4 is an alkanoyl, sulfonyl, carbonyloxy, carbamoyl or thiocarbamoyl group which may be substituted; X is formula: --CHO or --CH.sub.2 OB (wherein B is a hydrogen atom or a protecting group of hydroxyl group); m and n independently are an integer of 0 or 1; provided that R.sup.4 is an alkanoyl group substituted by aryl, a sulfonyl group substituted by aryl having more than 9 carbon atoms or by lower alkyl, or a carbamoyl or thiocarbamoyl group which may be substituted when R.sup.1 is an unsubstituted lower alkyl, arylalkyl on methylthioethyl group, R.sup.2 and R.sup.3 independently are a lower alkyl or arylalkyl, X is --CHO, m is 1 and n is 0 or 1, or a salt thereof.

Abstract: The present invention is directed to a method for cloning and producing the SfiI restriction endonuclease by 1) introducing the restriction endonuclease gene from S. fimbriatus into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SfiI restriction endonuclease activity; and 3) purifying the SfiI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SfiI restriction endonuclease activity.

Abstract: Recombinant avipox viral vectors which express heterologous polypeptides capable of assembling into defective nonself-propagating viral particles are disclosed. The recombinant avipox viruses can be used to produce significant amounts of the heterologous polypeptides in avian or non-avian cells. Preferably, the recombinant avipox virus is a fowlpox virus. The viral particles can also be used as immunogens and for targeted delivery of heterologous gene products and drugs.

Abstract: When a CPU switches the power supply to a fusing lamp to OFF from ON state while an exposing lamp stays on, the CPU suspends a feedback phase control of the exposing lamp and carries out a phase control of the exposing lamp based on the phase control data pre-stored in a RAM for a predetermined period before and after the switching. The exposing lamp is hardly affected by the variance in voltage caused by the switching of the power supply state to the fusing lamp, and therefore can emit exposing light in a constant amount to form an image. Thus, it becomes possible to produce a satisfactory copy image with substantially no inconsistency in copy density.

Abstract: Recombinant viral vectors which coexpress heterologous polypeptides capable of assembling into defective nonself-propagating viral particles are disclosed. The viral vectors as well as the viral particles can be used as immunogens and for targeted delivery of heterologous gene products and drugs.

Abstract: Expession of a target gene or transene in catecholaminergic cells of a transgenic mammal operably linked to the 5' flanlking sequence of a rat tyrosine hydroxylase gene is disclosed as well as immortalized catecholaminergic neuronal cell lines employing an oncogene operably linked to the 5' flanking sequence of a rat tyrosine hydroxylase gene.