Abstract: A method of in vitro fucosylation of selectin ligands on cord blood-derived hematopoietic stem cells for bone marrow transplantation is disclosed. In this method, an effective amount of an ?1,3-fucosyltransferase, e.g., ?1,3-fucosyltransferase VI, is used in vitro to treat cord blood-derived hematopoietic stem cells to convert non-functional PSGL-1 or other ligands on the cell surface into functional forms that bind selectins, especially P-selectin or E-selectin. The treated cells have enhanced effectiveness in reconstituting bone marrow in patients in need of such therapy.

Abstract: A valve assembly provides the ability for internal tank operations providing novel and unique methods for intricate measurement, monitoring, sampling, mixing, isolation, evaluation or other tasks desired inside tanks, vessels, piping or flow applications. The valve assembly is supported by the sidewall of the vessel and extends into a cavity of the vessel. The valve assembly including a barrel and a valve member. The barrel has a wall defining a chamber. The wall has at least one opening. The valve member is movable between a closed position and an open position.

Abstract: A decorative cover formed from a sheet of material having forming indicia thereon is disclosed. The forming indicia permits an operator to form the sheet of material into the decorative cover without the use of a mold, such as a flower pot or potted plant. A method for forming the sheet of material into the decorative cover is also disclosed.

Abstract: Tyrosylprotein sulfotransferases and nucleic acids encoding the tyrosyiprotein sulfotransferases are described. Dual isotopes of the enzyme and of the nucleic acids encoding said enzymes have been identified in human, mouse and C. elegans. The polypeptides and polynucleotides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational tyrosine sulfation of proteins or peptides produced within the expression system. The enzymes can be used to sulfate peptides or proteins requiring sulfation.

Abstract: A new class of synthetic glycosulfopeptides (GSPs) which have one or more sulfated tyrosine residues and a glycan linked to the peptide, the glycan preferably including a sialyl Lewisx group or a sialyl Lewisa group. In a preferred version the GSPs have an O-glycan comprising a &bgr;1, 6 linkage to a GalNAc. The present invention further contemplates in vitro methods of the synthesis of these GSPs without the use of the cells and methods of their use in vivo as powerful anti-inflammatory antithrombotic, or anti-metastatic compounds. The invention also contemplates a method of synthesizing oligosaccharides by cleaving the glycan from the GSP.

Abstract: Core 1 &bgr;3-galactosyl transferases and nucleic acids encoding the core 1 &bgr;3-galactosyl transferases are described. The enzymes and the nucleic acids encoding said enzymes have been identified in human, rat, mouse D. melanogaster and C. elegans. The polypeptides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational core 1 O-linked glycosylation of proteins or peptides produced within the expression system. The enzymes can be used to galactosylate, via a &bgr;3-linkage, an N-acetylgalactosamine linked to a serine, threonine or other O-linking amino acid on peptides or proteins requiring O-linked glycosylation.

Abstract: A welding helmet includes a connector for connecting the head gear and the protective shell and is comprised of an external finger knob allowing for user adjustment of the friction developed by the connector, a conical pivot stop having a conical surface, a strap member having a matching conical surface with the frictional forces being developed between these two conical surfaces, and a bolt which joins the parts of the connector and mounts them between the head gear and shell. A guide pin and guide slot in the stop and strap member control and guide the movement between these two parts as the shell is moved with respect to the user's head. A locator pin in the stop is inserted into one of a series of holes in the helmet to locate the angular orientation between the stop and the helmet, and thereby helps to fix the limits of relative movement between the shell and the user's head.

Abstract: A plant packaging system comprising a combination of a protective sleeve portion and a decorative cover portion having a base and skirt for packaging a potted plant. The protective sleeve can be detached from the decorative portion of the package system once the protective function of the sleeve has been completed. The sleeve has one or a plurality of horizontally positioned expansion elements for allowing the skirt portion to extend angularly from the base when the upper sleeve portion is detached.

Abstract: A spacing member for use with an alternator of the type having an electrical generating assembly housed in a frame with an external mounting surface to which a voltage regulator is adapted to be mounted. A brush assembly is connected to the voltage regulator and has a pair of brushes adapted to engage a pair of slip rings of the electric generating assembly when the brush assembly is positioned in a slip ring engaging position, the spacing member supports the voltage regulator in a spaced apart relationship relative to the mounting surface of the frame so as to provide an air flow passage between the voltage regulator and the mounting surface for enhancing heat transfer away from the voltage regulator and the spacing member supports the voltage regulator in a spaced apart relationship relative to the brush assembly so as to support the brush assembly in the slip ring engaging position.