Patents Represented by Attorney, Agent or Law Firm Elia J. Lambiris, Esq.
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Patent number: 6423524Abstract: The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.Type: GrantFiled: November 10, 1998Date of Patent: July 23, 2002Assignee: Novo Nordisk A/SInventors: Fred Hagen, Carsten Mailand Hjort, Sven Hastrup
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Patent number: 6372465Abstract: The present invention relates to variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A,L,I,Q,M,E,D; A399G; F397N,Y,E,Q; P395A,S; R360A,L,I,Q,M,E,D; K353Q,M; S402A,T,V,S; D292L; A501S; W350F,Y; V495A,T,V,S; K394 A,L,I,Q,M,E,D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.Type: GrantFiled: February 20, 2001Date of Patent: April 16, 2002Assignee: Novozymes A/SInventors: Allan Svendsen, Louise Jørgensen
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Patent number: 6365204Abstract: Disclosed is a process for preparing a dough or a baked product from the dough which process involves incorporating into the dough an anti-staling amylase and a phospholipase. The bread made by the combined use of an anti-staling amylase and a phospholipase has improved softness, both when eaten on the same day and when stored for several days after baking. There is no significant change in the taste or smell of the baked product.Type: GrantFiled: April 5, 1999Date of Patent: April 2, 2002Assignee: NovozymesInventors: Tina Spendler, Lone Nilsson, Claus Crone Fuglsang
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Patent number: 6346401Abstract: The present invention deals with a process for oxidation of a hydroxy group of C1 and/or C2 and/or C3 and/or C4 and/or C5 and/or C6 of a sugar monomer of an oligo- or a polysaccharide comprising contacting, in an aqueous medium, the oligo- or the polysaccharide with a phenol oxidizing enzyme and an enhancing agent, whereby an oligo- or a polysaccharide with altered characteristics compared to the native oligo- or polysaccharide is created.Type: GrantFiled: May 26, 1999Date of Patent: February 12, 2002Assignee: Novozymes A/SInventor: Jesper Vallentin Kierulff
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Patent number: 6306631Abstract: The present invention relates to a novel ppGpp synthetase and expression systems for improved production of proteins of interest in Gram-positive bacteria, involving use of the novel ppGpp synthetase.Type: GrantFiled: November 16, 1999Date of Patent: October 23, 2001Assignee: Novozymes A/SInventors: Jens Tonne Andersen, Stanislas Dusko Ehrlich
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Patent number: 6306813Abstract: Lipases having improved washing performance for use in detergents to improve the removal of fatty soiling, and having activity throughout a temperature range of 1 to 65° C., including an activity at 1° C. which is at least 20% the activity at 30° C., activity over a pH range of 8-12, and activity at high concentration of anionic surfactant and in the presence of bleach.Type: GrantFiled: September 11, 1998Date of Patent: October 23, 2001Assignee: Novozymes A/SInventor: Masahiro Suzuki
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Patent number: 6303752Abstract: The present invention relates to modified polypeptides with reduced respiratory allergenicity having polymeric molecules with a molecular weight from 100 up to 750 Da, coupled covalently to the parent polypeptide having a molecular weight from 5 to 100 kDa. The present invention also relates to industrial compositions comprising modified polypeptide with reduced respiratory allergenicity, skin care products, the use of modified polypeptides for reducing the allergenicity of industrial composition and products and finally a method for reducing the allergenicity of polypeptides.Type: GrantFiled: June 25, 1998Date of Patent: October 16, 2001Assignee: Novozymes A/SInventors: Arne Agerlin Olsen, Tine Muxoll Fatum, Heinz Josef Deussen, Erwin Ludo Roggen
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Patent number: 6300116Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.Type: GrantFiled: November 4, 1997Date of Patent: October 9, 2001Assignee: Novozymes A/SInventors: Claus von der Osten, Torben Halkier, Carsten Andersen, Peter Bauditz, Peter Kamp Hansen
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Patent number: 6298859Abstract: A process for preparing tobacco, which process comprises the steps of treating a tobacco material with a phenol oxidising enzyme, such as by extracting tobacco with a solvent to provide an extract and a residue; and treating the extract with a phenol oxidising enzyme such as a laccase. An improved tobacco product having a reduced amount of phenolic compounds. This is an alternative or a supplement to a process in which the phenolic compounds are adsorbed onto the insoluble carrier polyvinylpolypyrrolidone (PVPP). In preferred embodiments, the process includes further steps of removing the oxidised phenolic compound, adding adsorbents such as bentonite; removing and/or inactivating the enzyme; and concentrating the extract. Preferred phenol oxidising enzymes are peroxidases and laccases. The thus treated extract is advantageously re-combined with the tobacco residue and further processed to provide a tobacco article for smoking.Type: GrantFiled: July 7, 1999Date of Patent: October 9, 2001Assignee: Novozymes A/SInventors: Jesper Vallentin Kierulff, Ole Bill Jørgensen, Tomas Tage Hansen, Anthony James Knox, Yves De Grandpré
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Patent number: 6296672Abstract: The present invention relates to methods of dyeing a material which involve contacting the material with a dyeing system which comprises: (a) a mixture of (i) an aromatic diamine and (ii) one or more of a naphthol and an aminonaphthalene and (b) an oxidation system comprising (i) a hydrogen peroxide source and an enzyme exhibiting peroxidase activity or (ii) an enzyme exibiting oxidase activity on one or more of the compounds of mixture (a). The material may be a fabric, yarn, fiber, garment or film made of fur, hide, leather, silk or wool, or made of cationic polysaccharide, cotton, diacetate, flax, linen, lyocel, polyacrylic, synthetic polyamide, polyester, ramie, rayon, triacetate, or viscose.Type: GrantFiled: December 14, 1999Date of Patent: October 2, 2001Assignees: Novozymes A/S Patents, Novozymes North AmericaInventors: Martin Barfoed, Ole Kirk, Sonja Salmon
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Patent number: 6287826Abstract: The present invention relates to a process for the preparation of a glucose syrup wherein starch is treated with a Termamyl-like &agr;-amylase comprising a substitution in Val54 shown in SEQ ID NO: 2 or in the corresponding position in another Termamyl-like &agr;-amylase. The invention also relates to a glucose syrup obtainable by the process of the invention and the use thereof as ingredient in food products. An object of the invention is also to provide for the use of a Termamyl-like &agr;-amylase with a substitution in position Val54 using SEQ ID NO: 2 as the backbone or a corresponding position in another Termamyl-like &agr;-amylase for preparing glucose syrup.Type: GrantFiled: March 8, 1999Date of Patent: September 11, 2001Assignee: Novo Nordisk A/SInventors: Barrie Edmund Norman, Hanne Vang Hendriksen
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Patent number: 6270813Abstract: A process for preparing a dough or a baked product comprises adding an amylase to the dough in an amount which is effective to retard the staling of the bread. The amylase is an exo-amylase which hydrolyzes starch to form mainly mal-totriose.Type: GrantFiled: March 28, 2000Date of Patent: August 7, 2001Assignee: Novozymes A/SInventors: Jack Beck Nielsen, Thomas Schäfer
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Patent number: 6268197Abstract: A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.Type: GrantFiled: July 7, 1998Date of Patent: July 31, 2001Assignee: Novozymes A/SInventors: Martin Schulein, Helle Outtrup, Per Lina Jorgensen, Mads Eskelund Bjornvad
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Patent number: 6268343Abstract: The present invention relates to GLP-1 derivatives having a lipophilic substituent, pharmaceutical compositions comprising same, and methods of making an using same. The GLP-1 derivatives of the present invention have a protracted profile of action.Type: GrantFiled: February 26, 1999Date of Patent: July 31, 2001Assignee: Novo Nordisk A/SInventors: Liselotte Bjerre Knudsen, Per Olaf Huusfeldt, Per Franklin Nielsen, Niels C. Kaarsholm, Helle Birk Olsen, Søren Erik Bjørn, Freddy Zimmerdahl Pedersen, Kjeld Madsen
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Patent number: 6261828Abstract: A one-step process for combined desizing and “stone-washing” of dyed denim, wherein the denim is treated with an amylolytic enzyme in combination with a first abrading monocomponent endoglucanase and a second streak-reducing monocomponent endoglucanase.Type: GrantFiled: April 29, 1998Date of Patent: July 17, 2001Assignee: Novo Nordisk A/SInventor: Henrik Lund
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Patent number: 6255084Abstract: The invention relates to an isolated thermostable glucoamylase derived from Talaromyces emersonii suitable for starch conversion processes.Type: GrantFiled: November 24, 1998Date of Patent: July 3, 2001Assignee: Novozymes A/SInventors: Bjarne Rønfeldt Nielsen, Ruby Ilum Nielsen, Jan Lehmbeck
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Patent number: 6242237Abstract: The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme with galactanase activity, a method of producing the enzyme, an enzyme composition comprising the enzyme with galactanase activity, and the use of the galactanase enzyme and enzyme composition for a number of industrial applications.Type: GrantFiled: August 21, 1998Date of Patent: June 5, 2001Assignee: Novo Nordisk A/SInventors: Lene Venke Kofod, Markus Sakari Kauppinen, Lene Nonboe Andersen, Ib Groth Clausen, Anette Müllertz
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Patent number: 6232101Abstract: A method for causing gelling or increase of viscosity of an aqueous medium containing a gellable polymeric material having substituents with phenolic hydroxy groups comprises adding an oxidase, particularly a laccase, to the aqueous medium.Type: GrantFiled: October 28, 1996Date of Patent: May 15, 2001Assignee: Novozymes A/S PatentsInventors: Gitte Budolfsen, Hans Peter Heldt-Hansen
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Patent number: 6231621Abstract: The invention relates to substrates which develops color on oxidation of general formula (I). The invention also relates to the use of said substrates for dyeing, e.g. keratinous fibers, in particular hair, dyeing textiles, a dyeing composition and a method for dyeing keratinous fibers.Type: GrantFiled: March 18, 1999Date of Patent: May 15, 2001Assignee: Novozymes A/SInventors: Niels Henrik Sørensen, Ole Kirk, Rikke Lolck, Torben Desler
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Patent number: 6228630Abstract: An enzyme preparation comprising (a) first enzyme with xylanase activity and (b) a second enzyme having cellulotytic, xylanolytic or pectinolytic activity and are supported by the specification.Type: GrantFiled: June 22, 2000Date of Patent: May 8, 2001Assignee: Novo Nordisk A/SInventors: Lene Venke Kofod, MArkus Sakari Kauppinen, Stephan Christgau, Hans Peter Heldt-Hansen, Henrik Dalbøge, Lene Nonboe Andersen, Joan Qi Si, Tina Sejersgåard Jacobsen, Niels Munk, Anette Müllertz