Patents Represented by Attorney, Agent or Law Firm Elizabeth N. Spar
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Patent number: 6589743Abstract: The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid sequence, and cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid sequence in a sample.Type: GrantFiled: October 17, 2001Date of Patent: July 8, 2003Assignee: StratageneInventor: Joseph A. Sorge
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Patent number: 6551820Abstract: Plant expression vectors comprising at least two expression cassettes are provided which function to reduce transcriptional silencing of polynucleotide expression. Further, novel plant expression vectors for expression of immunogenic polypeptides, including HBsAg, are provided. The plant expression vectors can be used to produce immunogenic polypeptides, including HBsAg, in edible plant tissues. The edible plant tissues can be used to elicit an immune response in humans and animals when the plant tissues are consumed.Type: GrantFiled: December 23, 1999Date of Patent: April 22, 2003Assignee: Boyce Thompson Institute for Plant ResearchInventors: Hugh S. Mason, Yasmin Thanavala, Charles Joel Arntzen, Elizabeth Richter
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Patent number: 6548250Abstract: The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid sequence with a nucleic acid polymerase and cleaving the cleavage structure with a FEN nuclease to generate a cleaved nucleic acid fragment. The invention also relates to methods of detecting or measuring a target nucleic acid sequence, where the method includes forming a cleavage structure by incubating a target nucleic acid sequence with a nucleic acid polymerase, cleaving the cleavage structure with a FEN nuclease and detecting or measuring the release of a fragment.Type: GrantFiled: August 30, 2000Date of Patent: April 15, 2003Assignee: StratageneInventor: Joseph A. Sorge
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Patent number: 6528254Abstract: The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid sequence with a nucleic acid polymerase and cleaving the cleavage structure with a FEN nuclease to generate a cleaved nucleic acid fragment. The invention also relates to methods of detecting or measuring a target nucleic acid sequence, where the method includes forming a cleavage structure by incubating a target nucleic acid sequence with a nucleic acid polymerase, cleaving the cleavage structure with a FEN nuclease and detecting or measuring the release of a fragment.Type: GrantFiled: October 29, 1999Date of Patent: March 4, 2003Assignee: StratageneInventor: Joseph A. Sorge
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Patent number: 6503504Abstract: Disclosed herein is a method of delivering a bioactive compound to an organism that involves growing individual cells in vitro under conditions that allow the formation of an organized tissue, at least a subset of the cells containing a foreign DNA sequence which mediates the production of the bioactive compound; and implanting the organized tissue into the organism, whereby the bioactive compound is produced and delivered to the organism.Type: GrantFiled: July 17, 1997Date of Patent: January 7, 2003Assignee: Miriam HospitalInventor: Herman H. Vandenburgh
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Patent number: 6485960Abstract: The present invention relates to the polycystic kidney disease 1 (PKD1) gene and its nucleic acid sequence, mutations thereof in patients having PKD1-associated disorders, the protein encoded by the PKD1 gene or its mutants, and their uses in disease diagnosis and therapy.Type: GrantFiled: April 14, 1995Date of Patent: November 26, 2002Assignee: Medical Research CouncilInventors: Peter Charles Harris, Belen Peral, Christopher J. Ward, James Hughes, Martin Hendrik Breuning, Dorothea Johanna Maria Peters, Jeroen Hendrik Roelfsema, Julian Sampson, Dirkje Jorijntje Johanna Halley, Mark David Nellist, Lambertus Antonius Jacobus Janssen, Ajenne Lique Wilhelma Hesseling
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Patent number: 6469228Abstract: Disclosed are isolated transposable elements, or isolated DNA sequences which encode a transposase protein (or a portion of a transposase protein), The isolated transposable elements or the isolated DNA sequences being characterized by the ability to hybridize to the DNA sequence of Minos 1 under stringent hybridization conditions. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences. Such transposable are useful in methods for the stable introduction of a DNA sequence of interest into a eukaryotic cell. The sequence information disclosed herein is useful in the design of oligonucleotide primers which are useful for the isolation of related members of the Tc-1 family of transposable elements.Type: GrantFiled: May 9, 1994Date of Patent: October 22, 2002Assignee: Minos BiosystemsInventors: Charalambos Savakis, Gerald H. Franz, Athanasios Loukeris
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Patent number: 6380360Abstract: The present invention relates to the polycystic kidney disease 1 (PKD1) gene and its nucleic acid sequence, mutations thereof in patients having PKD1-associated disorders, the protein encoded by the PKD1 gene or its mutants, and their uses in disease diagnosis and therapy.Type: GrantFiled: March 31, 1998Date of Patent: April 30, 2002Assignee: Medical Research CouncilInventors: Peter Charles Harris, Belen Peral, Christopher J. Ward, James Hughes, Martin Hendrik Breuning, Dorothea Johanna Maria Peters, Jeroen Hendrik Roelfsema, Julian Sampson, Dirkje Jorijntje Johanna Halley, Mark David Nellist, Lambertus Antonius Jacobus Janssen, Ajenne Lique Wilhelma Hesseling