Abstract: The present invention relates to the finding and construction of fish insulin-like growth factor II (IGF-II) cDNAs which can be cloned and expressed in cells. This invention also relates to the production of biologically active fish IGF-II polypeptides by a gene expression system using fish IGF-II cDNAs. The fish IGF-II cDNAs have 1977 bp which transcribe into a prepeptide (signal peptide), and B, C, A, D, E domain peptides. The fish mature IGF-II is a single polypeptide containing the NH.sub.2 -B-C-A-D-COOH domains. The mature IGF-II polypeptide is 7 kDa in weight and has 70 amino acids. The fish recombinant IGF-II CDNA can be cloned and expressed in E. coli, yeast, baculovirus, and fish cells.

Abstract: A method of forming shallow junctions in a CMOS transistor is disclosed. The method comprises the steps of: (a) forming a diffusion source layer on a N-well region, a P-well region, field oxide layer, and the gates of a CMOS transistor; (b) forming a photoresist layer over the P-well region; (c) carrying out p-type ion implantation to dope a part of the diffusion source layer on the P-well region; (d) removing the photoresist layer on the P-well region; (e) forming a photoresist layer over the N-well region; (f) carrying out n-type ion implantation to dope the other part of the diffusion source layer on the N-well region; (g) removing the photoresist layer on the N-well region; and (h) oxidizing the diffusion source layer and driving the ions therein into the P-well and N-well regions to form shallow junctions, respectively. The present invention has several advantages. First, it is compatible with the conventional CMOS process.