Abstract: A method for isolating a desired protein which comprises: providing a host cell expressing the desired protein as an insoluble aggregate; culturing the host cell with an effective mount of Cu.sup.++ ; disrupting the host cell producing a lysate; incubating the insoluble fraction non-disulfide-bond reducing or non-copper competing chaotropic conditions to solubilize contaminants; separating the insoluble from the soluble fraction; and exposing the insoluble fraction to disulfide-bond reducing or copper competing chaotropic conditions to solubilize the desired protein.
Type:
Grant
Filed:
April 16, 1992
Date of Patent:
June 4, 1996
Assignee:
Chiron Corporation
Inventors:
Lawrence S. Cousens, Patricia Tekamp-Olson
Abstract: Shipping fever, and other stress-related diseases in livestock, are treated by administration of a microencapsulated composition comprising IL-2 conjugated with a polyoxyethylene polymer, and mixed with a release-modulating amount of humen serum albumin. The microcapsules are administered parenterally, and release an effective amount of conjugated IL-2 continuously over a period of 14-30 days. These microcapsules are also effective in the treatment of cancer in mammals.
Type:
Grant
Filed:
August 12, 1988
Date of Patent:
April 7, 1992
Assignee:
Cetus Corporation
Inventors:
Maninder Singh, Jack H. Nunberg, Thomas R. Tice, Michael E. Hudson, Richard M. Gilley, Terrance A. Taforo