Abstract: This invention relates to the isolation of a novel putative efflux gene from Pseudomonas mendocina. The putative efflux gene is useful for probing an organism's efflux system to gain an understanding of the mechanisms of solvent tolerance. The invention further provides a Pseudomonas mendocina strain deficient in this gene. This strain is unable to grow in the presence of chloramphenicol and, compared to the wildtype strain, grows slowly in the presence of high concentrations of PHBA.
Abstract: Novel reactions used to prepare phosphole and bisphosphole compounds are detailed. Novel phosphole compounds and metal coordination compounds of phosphole and bisphosphole compounds are also provided. These metal coordination compounds are useful as catalysts for the polymerization or olefins with carbon monoxide and for the polymerization of acrylic monomers.
Type:
Grant
Filed:
August 30, 2000
Date of Patent:
February 26, 2002
Assignee:
E. I. du Pont de Nemours and Company
Inventors:
Paul J. Fagan, George Yanwu Li, Zhibin Guan, Lin Wang
Abstract: This invention relates to an isolated nucleic acid fragment encoding a branched-chain amino acid degradation enzymes. The invention also relates to the construction of a chimeric gene encoding all or a portion of the branched-chain amino acid degradation enzymes, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the branched-chain amino acid degradation enzymes in a transformed host cell.
Type:
Grant
Filed:
July 29, 1999
Date of Patent:
February 19, 2002
Assignee:
E.I . du Pont de Nemours and Company
Inventors:
Rebecca E. Cahoon, William D. Hitz, Anthony J. Kinney, J. Antoni Rafalski