Patents Represented by Attorney, Agent or Law Firm Joe Liebeschuetz
-
Patent number: 6800439Abstract: Improved nucleic acid arrays are provided which have been prepared in a non-oxidizing atmosphere.Type: GrantFiled: February 7, 2001Date of Patent: October 5, 2004Assignee: Affymetrix, Inc.Inventors: Glenn McGall, Lisa Kajisa, Mark Trulson
-
Patent number: 6747143Abstract: A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.Type: GrantFiled: May 20, 2002Date of Patent: June 8, 2004Assignee: Affymetrix, Inc.Inventors: Lubert Stryer, Stephen P. A. Fodor, J. Leighton Read, Michael C. Pirrung
-
Patent number: 6660234Abstract: A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.Type: GrantFiled: May 15, 2002Date of Patent: December 9, 2003Assignee: Affymetrix, Inc.Inventors: Luburt Stryer, Stephen P. A. Fodor, J. Leighton Read, Michael C. Pirrung
-
Patent number: 6602986Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: November 15, 2000Date of Patent: August 5, 2003Assignee: Maxygen, Inc.Inventors: Willem P. C. Stemmer, Andreas Crameri
-
Patent number: 6566495Abstract: A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.Type: GrantFiled: December 17, 1999Date of Patent: May 20, 2003Assignee: Affymetrix, Inc.Inventors: Stephen P. A. Fodor, Lubert Stryer, J. Leighton Read, Michael C. Pirrung
-
Patent number: 6506603Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: November 18, 2000Date of Patent: January 14, 2003Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6444468Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: November 28, 2000Date of Patent: September 3, 2002Assignee: Maxygen, Inc.Inventors: Willem P. C. Stemmer, Andreas Crameri
-
Patent number: 6420175Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: January 15, 1999Date of Patent: July 16, 2002Assignee: Maxygen, Inc.Inventor: Willem P.C. Stemmer
-
Patent number: 6399383Abstract: The invention provides human papillomavirus vectors useful in gene therapy. Such a vector contains E1 and E2 coding regions from a benign or low-risk human papillomavirus operably linked to a promoter and enhancer, and an LCR region from a human papillomavirus comprising an origin of replication including binding sites for the E1 and E2 proteins. The invention further provides methods of using such vectors in gene therapy, methods of controlling expression using a patch, and methods of using such vectors to evolve drugs for stimulation of hair growth or alteration of hair color.Type: GrantFiled: October 27, 1998Date of Patent: June 4, 2002Assignee: Maxygen, Inc.Inventors: Doris Apt, Paul Khavari, William P. C Stemmer
-
Patent number: 6391552Abstract: The invention provides a number of strategies for transferring and/or evolving gene(s) associated with cellular DNA uptake so that they confer or enhance DNA-uptake capacity of a recipient cell. Evolution is achieved by recursive cycles of recombination and screening/selection. One such strategy entails evolving genes that confer competence in one species to confer either greater competence in that species, or comparable or greater competence in a second species. Another strategy entails evolving genes for use as components of cloning vector to confer enhanced uptake of the vector. Other strategies entail evolving viral receptors, viruses, and genes that mediate conjugal transfer.Type: GrantFiled: November 1, 1999Date of Patent: May 21, 2002Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6387702Abstract: The invention provides a number of strategies for transferring and/or evolving gene(s) associated with cellular DNA uptake so that they confer or enhance DNA-uptake capacity of a recipient cell. Evolution is achieved by recursive cycles of recombination and screening/selection. One such strategy entails evolving genes that confer competence in one species to confer either greater competence in that species, or comparable or greater competence in a second species. Another strategy entails evolving genes for use as components of cloning vector to confer enhanced uptake of the vector. Other strategies entail evolving viral receptors, viruses, and genes that mediate conjugal transfer.Type: GrantFiled: November 1, 1999Date of Patent: May 14, 2002Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6365408Abstract: A method of mutating a polynucleotide such that it has a desired or improved functional property is disclosed.Type: GrantFiled: January 4, 2000Date of Patent: April 2, 2002Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6358742Abstract: The invention provides a number of strategies for transferring and/or evolving gene(s) associated with cellular DNA uptake so that they confer or enhance DNA-uptake capacity of a recipient cell. Evolution is achieved by recursive cycles of recombination and screening/selection. One such strategy entails evolving genes that confer competence in one species to confer either greater competence in that species, or comparable or greater competence in a second species. Another strategy entails evolving genes for use as components of cloning vector to confer enhanced uptake of the vector. Other strategies entail evolving viral receptors, viruses, and genes that mediate conjugal transfer.Type: GrantFiled: November 1, 1999Date of Patent: March 19, 2002Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6291242Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: October 2, 1998Date of Patent: September 18, 2001Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6287861Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: August 12, 1998Date of Patent: September 11, 2001Assignee: Maxygen, Inc.Inventors: Willem P. C. Stemmer, Andreas Crameri
-
Patent number: 6277638Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: January 15, 1999Date of Patent: August 21, 2001Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6180406Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: June 17, 1998Date of Patent: January 30, 2001Assignee: Maxygen, Inc.Inventor: Willem P.C. Stemmer
-
Patent number: 6165793Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: May 8, 1998Date of Patent: December 26, 2000Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6132970Abstract: The invention is directed to methods of shuffling polynucleotide variants. The methods entail conducting a multi-cyclic polynucleotide extension process on partially annealed polynucleotide strands having sequences from the plurality of chosen polynucleotide variants, the polynucleotide strands having regions of similarity and regions of heterology with each other and being partially annealed through the regions of similarity, under conditions whereby one strand serves as a template for extension of another strand with which it is partially annealed to generate a population of shuffled polynucleotides. Shuffled polynucleotides are then selected or screened to identify a shuffled polynucleotide having a desired functional property.Type: GrantFiled: June 19, 1998Date of Patent: October 17, 2000Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer
-
Patent number: 6117679Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.Type: GrantFiled: March 25, 1996Date of Patent: September 12, 2000Assignee: Maxygen, Inc.Inventor: Willem P. C. Stemmer