Abstract: SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an inoculum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30.degree.-38.degree. C while continuing the agitation of the medium, periodically transferring the growing cells into fresh medium and continuing the incubation with agitation of the medium, whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.
Abstract: The invention relates to a process for the preparation of aldehydes by the hydroformylation of olefinically unsaturated compounds using a rhodium catalyst which catalyst is provided by (1) introducing rhodium into the reaction solution in the form of an ionic rhodium compound, the said ionic compound consisting of (a) a rhodium-containing cation having rhodium complexed with ligands other than halide, and (b) a non-coordinating anion; and (2) furnishing to the reaction solution at least two moles of a modifying ligand per mole of ionic rhodium compound where the modifying ligand is furnished either pre-coordinated in the said ionic rhodium compound or as a free compound, and where the modifying ligand is a tertiary organo phosphorous compound having from 3 to 90 carbon atoms, or a tertiary organo arsenic compound having from 3 to 90 carbon atoms, or a tertiary organo antimony compound having from 3 to 90 carbon atoms.
Type:
Grant
Filed:
April 4, 1975
Date of Patent:
October 4, 1977
Assignee:
Monsanto Company
Inventors:
Harold Burnham Tinker, Donald E. Morris
Abstract: Provisions are taken to counter nitrogen oxide fumes produced in catalyst preparation involving metal nitrate decomposition, as by including urea or another scavenger, or by delaying calcining until used as catalyst in treating auto exhaust.
Type:
Grant
Filed:
January 2, 1974
Date of Patent:
September 27, 1977
Assignee:
Monsanto Company
Inventors:
Wesley B. Argo, James W. Edwards, Albert P. Little, William M. Lumb
Abstract: A method of determining triglycerides in biological fluids which comprises reacting a biological fluid sample with a lipolytic enzyme to liberate free glycerol, reacting said free glycerol with a coupled enzyme series in an oxygenated aqueous solution containing ATP, GK, .alpha.-GPDH, NAD and an electron transfer agent, and measuring the uptake of oxygen by the oxidation of the resulting NADH with an oxygen-sensing electrode.
Type:
Grant
Filed:
December 15, 1975
Date of Patent:
August 30, 1977
Assignee:
Monsanto Company
Inventors:
Lloyd E. Weeks, John H. Johnson, Margaret J. Reents
Abstract: A method of determining creatine phosphokinase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing creatine phosphate, ADP, glucose, HK, G-6-PDH, NADP and an electron transfer agent and measuring the uptake of oxygen by the oxidation of the resulting NADPH with an oxygen-sensing electrode.
Abstract: A process for production of orthophenylphenol is disclosed. More specifically, the invention relates to the conversion of phenol or diphenyl ether into orthophenylphenol in a two-stage process in which dibenzofuran is an intermediate.
Abstract: A method of determining dopamine-.beta.-hydroxylase (D.beta.H) activity in biological fluids which comprises reacting a D.beta.H-specific substrate with a sample of said biological fluid in the presence of ascorbate, fumarate and oxygen and measuring the uptake of oxygen by the resulting hydroxylation with an oxygen-sensing electrode.
Abstract: The complexes [Ir(CO).sub.3 I], [HIr(CO).sub.3 I.sub.2 ], [HIr(CO).sub.2 I.sub.2 (H.sub.2 O)], [RIr(CO).sub.2 I.sub.2 ], [(RCO)Ir(CO).sub.2 I.sub.2 ] where R is an alkyl radical, are described. Processes for preparing the aforesaid complexes are described and in addition a new process for preparation of [Ir(CO).sub.3 I.sub.3 ] is described.
Type:
Grant
Filed:
July 29, 1974
Date of Patent:
June 14, 1977
Assignee:
Monsanto Company
Inventors:
Denis Forster, Arnold Hershman, Donald E. Morris
Abstract: Elemental phosphorus is oxidized via indirect electrolytic oxidation in an electrolysis medium containing elemental phosphorus, an aqueous solution of hydrogen halide, and a non-aqueous solvent to produce phosphorous acid, (HO).sub.2 HPO.
Abstract: A unitized solid phase kit for radioimmunoassay is disclosed. All of the necessary assay reagents are incorporated in a single tube wherein all phases of the assay procedure are performed, requiring only the addition of the patient's sample. Antibody is bound to the tube surface, while labelled antigen is also present but unbound. Storage in the absence of air and water results in the stabilization of the reagents such that the system can be stored for long periods.
Abstract: Water-insoluble, biologically active conjugates for use in a reactor core of a flow-through reactor are prepared by covalently bonding an enzyme directly to a fibrous dialdehyde cellulose such as cotton. Preferably the fibrous cellulose is placed in the reactor core of a flow-through reactor before or after it is treated with periodic acid solution to convert glucose units thereof to units containing dialdehyde groups. Thereafter, an enzyme solution is passed through the resultant dialdehyde cellulose to bind the enzyme to the cellulose.
Abstract: A method for preparation of heterocyclic fused ring compounds by dehydration and dehydrogenation of certain phenolic compounds in the presence of a cerium oxide catalyst is disclosed. Preferred phenolic compounds, catalytic materials, and reaction conditions also are described.
Abstract: Process for removing sulfur dioxide and nitrogen oxides from gaseous mixtures which comprises contacting such mixtures, in the presence of a reducing agent comprising carbon monoxide, hydrogen, or mixtures thereof, with a solid reagent comprising calcium oxide, magnesium oxide, or mixtures thereof at temperatures in excess of about 700.degree. C.
Abstract: A method for preparation of heterocyclic fused ring compounds by dehydrogenation of certain aromatic ether compounds in the presence of a cerium oxide catalyst is disclosed. Preferred aromatic ether compounds, catalytic materials, and reaction conditions also are described.
Abstract: A method for preparation of heterocyclic fused ring compounds by dehydration and dehydrogenation of certain phenolic compounds in the presence of water vapor and in the presence of a cerium oxide catalyst is disclosed. Preferred phenolic compounds, catalytic materials, and reaction conditions also are described.