Abstract: Modified animal erythropoietin polypeptides and uses thereof are provide.
Type:
Grant
Filed:
September 25, 2009
Date of Patent:
October 2, 2012
Assignee:
Ambrx, Inc.
Inventors:
Feng Tian, Anna-Maria A. Hays Putnam, Frank Song, Stephanie Chu, Joseph Sheffer, Richard S. Barnett, Marc E. Siladi, Kyle Atkinson, Darin Lee, Peter C. Canning
Abstract: The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH comprising non-naturally encoded amino acids and hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.
Type:
Grant
Filed:
October 17, 2007
Date of Patent:
May 15, 2012
Assignee:
Ambrx, Inc.
Inventors:
Ying Buechler, Ricky Lieu, Michael Ong, Stuart Bussell, Nick Knudsen, Ho Sung Cho
Abstract: Formulations of modified growth hormone polypeptides are provided, including human growth hormone polypeptides with one or more non-naturally encoded amino acids with an increased half-life.
Type:
Grant
Filed:
October 25, 2007
Date of Patent:
May 15, 2012
Assignee:
AMBRX, Inc.
Inventors:
Anna-Maria Hays, Ying Buechler, David C. Litzinger
Abstract: Modified human interferon polypeptides and uses thereof are provided, including PEGylated human interferon polypeptides with one or more non-naturally encoded amino acids.
Type:
Grant
Filed:
October 26, 2007
Date of Patent:
January 10, 2012
Assignee:
Ambrx, Inc.
Inventors:
Anna-Maria A. Hays, Bruce E. Kimmel, Ho Sung Cho, Bee-Cheng Sim, David C. Litzinger, Roberto Mariani, Vadim Kraynov, Nick Knudsen, Thomas O. Daniel, Alan Koder, Stuart Bussell, Junjie Liu, Zhenwei Miao, Theresa Morrow
Abstract: The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.
Type:
Grant
Filed:
December 21, 2005
Date of Patent:
December 20, 2011
Assignee:
AMBRX, Inc.
Inventors:
Ying Buechler, Ricky Lieu, Michael Ong, Stuart Bussell, Nick Knudsen, Ho Sung Cho
Abstract: The invention provides water-soluble compounds that include a polymer and at least one terminal azide or acetylene moiety. Also provided are highly efficient methods for the selective modification of proteins with PEG derivatives, which involves the selective incorporation of non-genetically encoded amino acids, e.g., those amino acids containing an azide or acetylene moiety, into proteins in response to a selector codon and the subsequent modification of those amino acids with a suitably reactive PEG derivative.
Abstract: The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH comprising non-naturally encoded amino acids and hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.
Type:
Grant
Filed:
October 17, 2007
Date of Patent:
June 14, 2011
Assignee:
Ambrx, Inc.
Inventors:
Ying Buechler, Ricky Lieu, Michael Ong, Stuart Bussell, Nick Knudsen, Ho Sung Cho
Abstract: The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH comprising non-naturally encoded amino acids and hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process of the present invention is also useful for purification of hGH linked to polymers or other molecules.
Type:
Grant
Filed:
January 31, 2008
Date of Patent:
May 24, 2011
Assignee:
AMBRX, Inc.
Inventors:
Ying Buechler, Ricky Lieu, Michael Ong, Stuart Bussell, Nick Knudsen, Ho Sung Cho
Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs.