Abstract: The present invention relates to compositions and methods for delivery of nucleic acids. In particular, the invention provides a polymeric delivery formulation including a nucleic acid to be transfected into a host cell, formulated in a biodegradable polymer having phosphorous-based linkages.
Type:
Grant
Filed:
June 18, 1999
Date of Patent:
April 15, 2003
Assignee:
Johns Hopkins University School of Medicine
Inventors:
Hai-Quan Mao, Kevin Y. Lin, Bart S. Hendriks, Kam W. Leong, Michael F. Haller
Abstract: A method and device to assist in the determination of protein domain boundaries is described. In particular the device is designed to provide a high throughput of proteolytic digestion of proteins to identify domains and their boundaries, for use in protein structure determination, in a manner that is amenable to automation. Proteases are immobilized in a convenient format such as a microtitre plate and preferably arranged in a matrix thereby allowing for simultaneous degradation of a protein by a number of proteases at a number of concentrations.