Abstract: The present invention relates to nucleic acid sequences from the bacterium, Myxococcus xanthus and, in particular, to genomic DNA sequences. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins and fragments of proteins. In addition, proteins and fragments of proteins so encoded and antibodies capable of binding the proteins are encompassed by the present invention. The invention also encompasses oligonucleotides including primers, e.g. useful for amplifying nucleic acid molecules, and collections of nucleic acid molecules and oligonucleotides, e.g. in microarrays. The invention also provides constructs and transgenic cells and organisms comprising nucleic acid molecules of the invention.

Abstract: The present invention is in the field of bioinformatics, particularly as it pertains to determining the associations of biological elements. More specifically, the present invention relates to the determination of associations among a set of biological elements using an algorithm that is capable of generating a Steiner tree.

Abstract: The present invention is in the field of plant biochemistry, particularly as it pertains to the &bgr;-oxidation pathway. More specifically, the invention relates to nucleic acid molecules that encode proteins and fragments of proteins associated with the b-oxidation pathway, the proteins and fragments of proteins so encoded, and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, proteins, and fragments of proteins.

Abstract: Arrays of polynucleotide or polypeptide target molecules immobilized on a surface of a substrate where the target molecules are arranged in the array according to intensity of organism expression of cognate probe molecules which hybridize to the target molecules. For instance, target molecules having a higher than average indicia of hybridization, e.g. at least a factor of 2, are segregated at a peripheral region of the substrate and at a lower surface density. Preferred arrays can contain animal, plant or microorganism target molecules including Aspergillus nidulans. Diagnostic arrays can comprise targets from mixed species, e.g. human, mouse and virus; plant breeding arrays can comprise targets from mixed plants, e.g. Arabidopsis thaliana, maize, soy, cotton, wheat, rice, canola and potato.

Abstract: In one aspect the present invention relates to the use of viral promoters in the expression of chimeric genes in plant cells. In another aspect this invention relates to chimeric genes which are capable of being expressed in plant cells, which utilize promoter regions derived from viruses which are capable of infecting plant cells. One such virus comprises the cauliflower mosaic virux (CaMV). Two different promoter regions have been derived from the CaMV genome and ligated to heterologous coding sequences to form chimeric genes. These chimeric genes have been shown to be expressed in plant cells. This invention also relates to plant cells, plant tissue, and differentiated plants which contain and express the chimeric genes of this invention.

Abstract: The invention provides plant cyclophilin promoters that direct efficient expression of contiguous structural coding sequences in essentially all plant cells and plant organs of transgenic plants. In addition, chimeric genes containing the plant cyclophilin promoters of the invention and vectors comprising the plant cyclophilin promoters and chimeric genes of the invention are taught herein.

Abstract: This invention relates to chimeric genes which are capable of being expressed in plant cells. Such genes contain (a) a promoter region derived a gene which is expressed in plant cells, such as the nopaline synthase gene; (b) a coding or structural sequence which is heterologous with respect to the promoter region; and (c) an appropriate 3′ non-translated region. Such genes have been used to create antibiotic-resistant plant cells; they are also useful for creating herbicide-resistant plants, and plants which contain mammalian polypeptides.

Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, and B.t.k. HD-73.

Abstract: A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.

Abstract: In one aspect the present invention relates to the use of viral promoters in the expression of chimeric genes in plant cells. In another aspect this invention relates to chimeric genes which are capable of being expressed in plant cells, which utilize promoter regions derived from viruses which are capable of infecting plant cells. One such virus comprises the cauliflower mosaic virux (CaMV). Two different promoter regions have been derived from the CaMV genome and ligated to heterologous coding sequences to form chimeric genes. These chimeric genes have been shown to be expressed in plant cells. This invention also relates to plant cells, plant tissue, and differentiated plants which contain and express the chimeric genes of this invention.

Abstract: 3-Hydroxysteroid oxidase controls insects, particularly lepidopterans and boll weevil. Genes encoding for this enzyme may be cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of controlling insect infestation.

Abstract: Novel 3,4,4-trifluorobutenoic acid derivatives of the formula ##STR1## wherein Q is --(C.dbd.W)--R.sub.2 wherein W is O or S, and R.sub.2 is an aliphatic or aromatic group when W.dbd.O; --OR.sub.50 ; --SR.sub.50 ; or --NR.sub.50 R.sub.60 wherein R.sub.60 is hydrogen or R.sub.50 ; NR.sub.7 R.sub.8 ; a heterocyclic group; phenyl substituted by alkyl; or any agronomically acceptable salt thereof having usefulness as nematocides, insecticides and acaricides.

Abstract: A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, B.t.k. HD-73, B.t. tenebrionis, B.t. entomocidus, 2 protein of B.t.k. HD-1, and the coat protein of potato leaf roll virus.

Abstract: A method for producing genetically transformed plants exhibiting toxicity to Coleopteran insects is disclosed. In another aspect, the present invention embraces chimeric plant genes, genetically transformed cells and differentiated plants which exhibit toxicity to Coleopteran insects. In yet another aspect, the present invention embraces bacterial cells and plant transformation vectors comprising a chimeric plant gene encoding a Coleopteran toxin protein of Bacillus thuringiensis.

Abstract: A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.

Abstract: In one aspect the present invention relates to the use of viral promoters in the expression of chimeric genes in plant cells. In another aspect this invention relates to chimeric genes which are capable of being expressed in plant cells, which utilize promoter regions derived from viruses which are capable of infecting plant cells. One such virus comprises the cauliflower mosaic virus (CaMV). Two different promoter regions have been derived from the CaMV genome and ligated to heterologous coding sequences to form chimeric genes. These chimeric genes have been shown to be expressed in plant cells. This invention also relates to plant cells, plant tissue, and differentiated plants which contain and express the chimeric genes of this invention.

Abstract: This present invention describes genetically transformed lettuce cells and transgenic lettuce plants which exhibit toxicity to Lepidopteran larvae.

Abstract: Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphosate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a conserved sequence found between positions 80 and 120 in the mature wild-type EPSP synthase.

Abstract: A process for producing an organic compound from a hydrocarbon containing feedstock. The feedstock is divided into two fractions. The first is subject to a primary steam reforming reaction. The reaction product is then combined with the second fraction and reacted with a free oxygen-rich gas in a secondary reforming reactor to form a synthesis gas having a ratio ofH.sub.2 /[2(CO)+3(CO.sub.2)]between 0.80 and 1.00. The synthesis gas is then mixed with a hydrogen-rich stream, which has been separated from a purge gas from the synthesis loop, to form a final synthesis gas. The final synthesis gas is injected into a synthesis loop in which the desired organic compound is formed. The purge gas extracted from the loop is subjected to a physical separation to form a hydrogen-rich gas stream and a residual gas stream. A portion of the hydrogen-rich stream is recycled to form the final synthesis gas.

Abstract: An ammonia production process in which excess nitrogen and traces of carbon oxides are removed from raw ammonia synthesis gas firstly by application of partial condensation and secondly by application of washing action provided by carbon-oxides-free liquified gas, rich in nitrogen, which is derived from a cryogenic process used preferentially for separation of hydrogen from the ammonia synthesis loop purge gas as produced in processes which use excess of nitrogen above stoichiometric requirements in the circulating gas in the synthesis loop.