Abstract: A method for automatically focusing on a slide includes the steps of locating a coverslip, acquiring images from predetermined focal depths in the slide, and starting at an initial focal depth proximate the surface of the coverslip. A set of predetermined characteristics within each of the images are measured to generate at least one image measurement for each of the plurality of images. A focus measure is computed for each of the images, where each focus measure is a function of at least one image measurement. A best focus location is determined relative to a focal depth where an acquired image has a highest focus measure.

Abstract: A method for single image based image contrast quality evaluation is used in a computer controlled image system including an automated microscope for acquiring images. An image is acquired. An image contrast score is computed for the image. An image reliability score is computed for the image. The image contrast score and image reliability score are accumulated and combined to produce a contrast quality score. The contrast quality score is compared to a predetermined limit so as to produce a quality of processing result.