Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
Type:
Grant
Filed:
July 19, 2004
Date of Patent:
August 5, 2008
Assignee:
Invitrogen Corporation
Inventors:
James L. Hartley, Michael A. Brasch, Gary F. Temple, Donna K. Fox
Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.
Type:
Grant
Filed:
December 11, 2000
Date of Patent:
July 1, 2008
Assignee:
Invitrogen Corp.
Inventors:
David Cheo, Michael A. Brasch, Gary F. Temple, James L. Hartley, Devon R. N. Byrd
Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.
Type:
Grant
Filed:
August 14, 2003
Date of Patent:
April 1, 2008
Assignee:
Invitrogen Corp.
Inventors:
David Cheo, Michael A. Brasch, Gary F. Temple, James L. Hartley, Devon R. N. Byrd