Abstract: The present invention provides a method and device for conducting a rapid in vitro enzyme immunoassay test for the direct and qualitative detection of two or more viral antigens from specimens of symptomatic patients. The method for immunoassay of viral antigens is performed on a membrane. Non-immunological capture of viral antigens takes place by absorption onto the membrane. Captured antigen binds to a detection reagent that includes a label conjugated to a specific antibody. The test is a differentiated test such that two or more viral antigens may be distinguished from each other in a single test. The invention also includes a kit for performing an assay in accordance with the method of the present invention, wherein the kit comprises the device of the present invention.
Type:
Grant
Filed:
December 8, 2000
Date of Patent:
May 30, 2006
Assignee:
Becton Dickinson and Company
Inventors:
Robert C. Fletcher, Alvydas J. Ozinskas, Kenneth Kopher, John Gill