Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

Abstract: A method for producing an L-amino acid, such as L-histidine, L-threonine, L-lysine, L-glutamic acid, and L-tryptophan, using bacterium belonging to the genus Escherichia which has increased expression of genes, such as those of the xylABFGHR locus, which encode the xylose utilization enzymes, is disclosed. The method includes cultivating the L-amino acid producing bacterium in a culture medium containing xylose, and collecting the L-amino acid from the culture medium.

Abstract: L-glutamic acid is produced by culturing in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source a microorganism that can metabolize a carbon source at a specific pH, and wherein said microorganism has an ability to accumulate L-glutamic acid in said medium in an amount which exceeds the the saturation concentration of L-glutamic acid, wherein the pH of said medium is controlled so that L-glutamic acid precipitates.

Abstract: A target substance is produced by culturing a bacterium which has the ability to produce the target substance in a medium to cause accumulation of said target substance in the medium and collecting the target substance from the medium, wherein the bacterium is modified so that a system for uptake of a byproduct of the target substance or a substrate for a biosynthesis system of the target substance into the bacterial cell.

Abstract: O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genus Escherichia which harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.

Abstract: A bacterial strain of Escherichia coli is described which produces L-threonine, and is obtained by a process comprising transduction by bacteriophage P1 which bears a transposon which inactivates threonine dehydrogenase activity, and isolation of a transductant lacking threonine dehydrogenase activity.

Abstract: A bacterium belonging to the genus Escherichia and having an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing an expression amount of an L-amino acid excretion protein, and a method for producing the L-amino acid using the bacterium.

Abstract: The present invention provides polypeptides and polynucleotides involved in C1 assimilation in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Abstract: A plasmid which is able to be isolated from Corynebacterium thermoaminogenes, and which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

Abstract: A gene encoding a protein constituting sucrose PTS of coryneform bacterium is provided by amplifying a region existing downstream from sucrase gene in coryneform bacterium by the cassette-ligation mediated PCR to obtain DNA encoding sucrose PTS enzyme II, which is a protein defined in the following (A) or (B): (A) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing; (B) a protein which has the amino acid sequence of SEQ ID NO: 2 in Sequence Listing including substitution, deletion, insertion, addition or inversion of one or several amino acids, and an activity for binding to sucrose.

Abstract: A bacterium which has an ability to produce an amino acid and in which a novel gene (rhtB) coding for a protein having an activity of making a bacterium having the protein L-homoserine-resistant is enhanced, is cultivated in a culture medium to produce and accumulate the amino acid in the medium, and the amino acid is recovered from the medium.

Abstract: Disclosed herein is a method of purifying L-glutamic acid by transformation recrystallization which method comprises maintaining crude crystals of L-glutamic acid containing ? crystals of L-glutamic acid in an aqueous solvent at a temperature of from 50° C. to the boiling point of said aqueous solvent in the coexistence of active carbon until about 30% or more of the crystals of L-glutamic acid have been transformed into ? crystals thereof, the amount of said aqueous solvent being an amount not more than the amount sufficient (i.e., an amount insufficient) to form a saturated solution of said crystals of L-glutamic acid, according to which method purified crystals of L-glutamic acid can be obtained extremely rapidly and in high yields, and remarkably conveniently and easily on an industrial scale, as compared with the conventional transformation recrystallization method which is a hitherto common purification method of L-glutamic acid.

Abstract: An Escherichia coil mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library of Bacillus methanolicus, a transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.

Abstract: A system for automatically creating databases containing industry, service, product and subject classification data, contact data, geographic location data (CCG-data) and links to web pages from HTML, XML or SGML encoded web pages posted on computer networks such as the Internet or Intranets. The web pages containing HTML, XML or SGML encoded CCG-data, database update controls and web browser display controls are created and modified by using simple text editors, HTML, XML or SGML editors or purpose built editors. The CCG databases may be searched for references (URLs) to web pages by use of enquiries which reference one or more of the items of the CCG-data. Alternatively, enquiries referencing the CCG-data in the databases may supply contact data without web page references. Data duplication and coordination is reduced by including in the web page CCG-data display controls which are used by web browsers to format for display the same data that is used to automatically update the databases.