Patents Represented by Attorney Stacev R. Sias

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  • Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
    Patent number: 5210015
    Abstract: The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
    Type: Grant
    Filed: August 6, 1990
    Date of Patent: May 11, 1993
    Assignee: Hoffman-La Roche Inc.
    Inventors: David H. Gelfand, Pamela M. Holland, Randall K. Saiki, Robert M. Watson