Abstract: The present invention relates to methods of reducing the rate at which a cotton-containing fabric becomes harsh or of reducing the harshness of a cotton-containing fabric, comprising treating the cotton-containing fabric with a cellulase preparation obtained from a fungus, wherein the cellulase preparation comprises a substantially homogeneous endoglucanase component which: (a) has a pH optimum of about 7.5-10.0; (b) exhibits a CMC endoase activity of at least about 10 CMC-endoase units per mg of total protein; (c) exhibits a CAVU activity of at least about 50% at a pH of 7.0-12.0; and (d) has essentially no cellobiohydrolase activity; wherein at least about 50% by weight of the total cellulase content of the cellulase preparation is the endoglucanase component.