Abstract: The present invention is based on the discovery that nitric oxide (NO) is an important growth regulator in an intact developing organism. In particular, the present invention relates to a method of increasing in a mammal a population of hematopoietic stem cells in bone marrow which are capable of undergoing normal hematopoiesis and differentiation, wherein the bone marrow is contacted with an inhibitor of NO, such as an inhibitor of nitric oxide synthase (NOS), thereby producing bone marrow having an increased population of hematopoietic stem cells which are capable of undergoing normal hematopoiesis and differentiation. The present invention also relates to a method of increasing a population of cells in S phase in a tissue of a mammal, comprising contacting the tissue with an inhibitor of NO, such as an inhibitor of NOS. The invention also pertains to a method of regenerating tissue in an adult mammal comprising contacting a selected tissue (e.g.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells are characterized by their ability to enhance the immune system (e.g., increase the activity of T and B lymphocytes and natural killer cells) in a mammal, said ability resulting from their having been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength. Also included are methods of making and using these compositions.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells are characterized by their ability to improve kidney functions (e.g., restoring urine secretion and/or lowering blood urea nitrogen, serum proteinuria, and/or serum creatinine levels) in a subject as a result of having been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength. Also included are methods of making such compositions.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to increase the capability of said plurality of yeast cells to improve the memory of a mammal. Also included are methods of making such compositions.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells are characterized by their ability to normalize the serum level of GPT, AP and/or LDH-5 in a mammal, said ability resulting from their having been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength. Also included are methods of making and using these compositions.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells are characterized by their ability to treat male hormone deficiency in a subject as a result of having been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength. Also included are methods of making such compositions.

Abstract: The present invention relates methods of generating infectious negative-strand virus in host cells by an entirely vector-based system without the aid of a helper virus. In particular, the present invention relates methods of generating infectious recombinant negative-strand RNA viruses intracellularly in the absence of helper virus from expression vectors comprising cDNAs encoding the viral proteins necessary to form ribonucleoprotein complexes (RNPs) and expression vectors comprising cDNA for genomic viral RNA(s) (vRNAs) or the corresponding cRNA(s). The present invention also relates to methods of generating infectious recombinant negative-strand RNA viruses which have mutations in viral genes and/or which express, package and/or present peptides or polypeptides encoded by heterologous nucleic acid sequences.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells are characterized by their ability to increase secretion of gastric juice or alleviate gastric ulcer in a mammal, said ability resulting from their having been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength. Also included are methods of making and using these compositions.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to substantially increase the capability of said plurality of yeast cells to convert biologically available phosphorus in a culture medium into their own biomass. Also included are methods of making such compositions.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to substantially increase the capability of said plurality of yeast cells to suppress the growth of algae or decompose debris of algae. Also included are methods of making such compositions.

Abstract: Compositions comprising a plurality of yeast cells which have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to substantially increase the capability of said plurality of yeast cells to degrade environmental toxins, such as antibiotics and organic solvents, in a culture medium. Also included are methods of making such compositions.

Abstract: Compositions comprising a plurality of yeast cells, wherein said plurality of yeast cells have been cultured in the presence of an alternating electric field having a specific frequency and a specific field strength for a period of time sufficient to substantially increase the capability of said plurality of yeast cells to convert bio-available nitrogen in a culture medium into their own biomass. Also included are methods of making such compositions.