Patents Assigned to AbClonal Science, Inc.
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Patent number: 11821010Abstract: The invention includes a mutant Taq polymerase, which can significantly extend and amplify a target sequence where the extension conditions are time limited to as little as one second. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.Type: GrantFiled: November 30, 2020Date of Patent: November 21, 2023Assignee: AbClonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun
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Patent number: 11788082Abstract: A number of T4 DNA ligase mutants exhibiting enhanced ligation activity at temperatures above 37° C. compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. T4 Ligase catalyzes the formation of phosphodiester bonds between the 5? and 3? ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.Type: GrantFiled: February 22, 2022Date of Patent: October 17, 2023Assignee: ABclonal Science, Inc.Inventors: Zhenyu Zhu, Aine Quimby, Dapeng Sun, Alicia DiCicco
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Patent number: 11781126Abstract: Proteases are enzymes which hydrolyze protein enzymes, eliminating their activity. The present invention exploits the hydrolyzing activity of proteases including proteinase K, endoproteinase LysC and/or trypsin to control the activity of restriction enzymes and/or eliminate or reduce production of unwanted DNA or RNA fragments (known as star activity).Type: GrantFiled: November 12, 2022Date of Patent: October 10, 2023Assignee: ABclonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun, Aine Quimby, Michaela Shottes
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Patent number: 11767524Abstract: Disclosed is a product and process, wherein one adds a N-terminal Histamine-Maltose Binding Protein (“MBP”) tag to endonucleases, including restriction endonucleases like Hind III, and binds the tagged fusion protein to a solid support, preferably beads, once the enzyme has digested oligonucleotides in solution, in order to arrest further digestion. Preferred beads for binding the tagged enzyme are magnetic beads, which can easily be removed from solution by binding to a support and then removing it, or can be accumulated by magnetic attraction in a particular region. More preferred are magnetic beads bound to iminodiacetic acid or nitrilotriacetic acid.Type: GrantFiled: September 22, 2020Date of Patent: September 26, 2023Assignee: ABclonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun, Michaela Shottes
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Patent number: 11746142Abstract: Disclosed is producing recombinant SARS-CoV-2 spike protein in a pre-fusion state, using furin knock out or knockdown mammalian cells (such as HEK293, CHO or other mammalian cells) and using them to generate antibodies and related binding agents. The antibodies/binding agents can be used in SARS-CoV-2 detection assays or in diagnosis of active or prior infection with SARS-CoV-2; in prophylaxis or as a therapeutic; or for prophylactic or therapeutic use against coronaviruses related to SARS-CoV-2.Type: GrantFiled: April 28, 2021Date of Patent: September 5, 2023Assignee: ABCLONAL SCIENCE, INC.Inventors: Yang Xiang, Yan Tan
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Patent number: 11685913Abstract: A number of T4 DNA ligase mutants exhibiting enhanced ligation activity in the presence of high salt concentrations compared to the wild-type ligase were engineered, characterized, and selected via gel electrophoresis of ligation products from a standard ligation assay. Ligase catalyzes the formation of phosphodiester bonds between the 5? and 3? ends of complementary cohesive ends or blunt ends of duplex DNA, a process that is vital to numerous molecular biology processes including cloning and sequencing.Type: GrantFiled: February 18, 2022Date of Patent: June 27, 2023Assignee: AbClonal Science, Inc.Inventors: Alicia DiCicco, Zhenyu Zhu, Aine Quimby
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Patent number: 11649441Abstract: Disclosed are Taq DNA polymerase mutants which exhibit enhanced efficiency in qPCR compared to the wild type Taq DNA polymerase. Such mutants include: V62S, V64S, A70F, F73A, A77F, P253G, E255K, D257R, A259F, A271F, L288S, E289K, S357I, L361S, L376S, P382G, T385I, G418P, R419D, E421K, L461S, A472F, E497K, L498S, E524K, D551R, R556D, S679I, L789S, E189K/E507K/E742K (See Sequence Listing Guide for the mutants' amino acid and protein sequences).Type: GrantFiled: September 9, 2021Date of Patent: May 16, 2023Assignee: ABCLONAL SCIENCE, INC.Inventors: Zhenyu Zhu, Dapeng Sun, Aine Quimby
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Patent number: 11613738Abstract: The invention includes a mutant Taq polymerase or biologically active fragment thereof, which can significantly extend and amplify a target sequence in comparison to wild type Taq polymerase, where the extension mixture includes asymmetrical cyanine dye, including SYBR Green I. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.Type: GrantFiled: May 11, 2020Date of Patent: March 28, 2023Assignee: ABCLONAL SCIENCE, INC.Inventors: Zhenyu Zhu, Dapeng Sun
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Publication number: 20230033390Abstract: Disclosed is a device for angling a multi-well plate during shaking on a typical low speed laboratory incubator-shaker to facilitate cell growth. An assembly with a holder for a deep-well multi-well plate to attach it to a base has an adjustable-angle joint. The base is attached to a conventional incubator shaker tray via screws or a sticky pad. The adjustable-angle joint allows maintaining the desired angle of the plate, even at full shaking speed.Type: ApplicationFiled: August 2, 2021Publication date: February 2, 2023Applicant: AbClonal Science, Inc.Inventors: Yang Xiang, Alexa Vahey, Aine Quimby
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Patent number: 11512296Abstract: Proteases are enzymes which hydrolyze protein enzymes, eliminating their activity. The present invention exploits the hydrolyzing activity of proteases including proteinase K, endoproteinase LysC and/or trypsin to control the activity of restriction enzymes and/or eliminate or reduce production of unwanted DNA or RNA fragments (known as star activity).Type: GrantFiled: February 9, 2021Date of Patent: November 29, 2022Assignee: ABclonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun, Aine Quimby, Michaela Shottes
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Patent number: 11168312Abstract: The invention includes a mutant Taq polymerase, which can effectively amplify a target sequence under conditions of salt concentration(s) similar to body fluids, including blood, serum or plasma preserved with sodium citrate. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.Type: GrantFiled: March 9, 2020Date of Patent: November 9, 2021Assignee: ABclonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun, Weiling Peng
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Patent number: 10947518Abstract: Proteases are enzymes which hydrolyze protein enzymes, eliminating their activity. The present invention exploits the hydrolyzing activity of proteases including proteinase K, endoproteinase LysC and/or trypsin to control the activity of restriction enzymes and/or eliminate or reduce production of unwanted DNA or RNA fragments (known as star activity).Type: GrantFiled: October 2, 2020Date of Patent: March 16, 2021Assignee: ABclonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun, Aine Quimby, Michaela Shottes
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Patent number: 10920202Abstract: Disclosed is an engineered thermolabile mutant of Serratia Marcescens Nuclease. In the same buffer as used for optimal enzyme activity, after 60° C. for 20 min, while the wild type retained 12.5% activity, the thermolabile mutant R146D/D156R/D229R/D245R (SEQ ID NO: 12, without the first 21 amino acids, which are a signal peptide) retained only 0.39% activity. Heat inactivation of the mutant, when it is used for protein purification, can be used after a period when substantial DNA in a protein has been degraded, but before unwanted degradation takes place.Type: GrantFiled: August 20, 2020Date of Patent: February 16, 2021Assignee: AbClonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun, Michaela Shottes
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Patent number: 10865441Abstract: The invention includes a mutant Taq polymerase, which can significantly extend and amplify a target sequence where the extension conditions are time limited to as little as one second. The mutant Taq polymerase, or a biologically active fragment thereof, has one or more substitutions differing from the wild type as shown in Table I.Type: GrantFiled: March 9, 2020Date of Patent: December 15, 2020Assignee: AbClonal Science, Inc.Inventors: Zhenyu Zhu, Dapeng Sun