Abstract: The disclosed synthesis system is based on the idea of designing a synthesis and treatment procedure, substrates and anchor groups which enable biomolecules to be simultaneously produced in an entirely automatic manner. By using a pipetting robot to dispense the reagents, the reaction column can be arranged in a format suitable for subsequent treatment. For a pipetting robot to carry out even water-sensitive or air-sensitive synthesis protocols, certain structural measures must be taken. The operation principle of the automated and the synthesis sequence are described below as an example of a possible solution. The automation can work with conventional substrates and reagents. Handling, however, is simplified by new, specially adapted substrates and anchor groups. A special, simultaneous purification and aliquot portioning process improves product quality and makes the device easier to use.
Type:
Grant
Filed:
November 30, 1999
Date of Patent:
February 11, 2003
Assignees:
Gesellschaft fuer Biotechnologische Forschung mbH
(GBF), Abimed Analysen-Technik GmbH, IMB Institut fuer Molekulare Bio technologie e.v.
Inventors:
Ronald Frank, Stefan Matysiak, Olaf Schreuer, Heinrich Gausepohl, Andre Rosenthal