Abstract: A capsule (100) comprising a housing (110) in which are disposed nanofluidic biosensors (120), a fluid connecting element (140), a filter (150) and a cover (160) is described. The capsule (100) allows the analysis of a fluid sample (300) that would be deposited in the capsule system (100) by a pipette (400). The fluid sample (300) is filtered when passing through a filter (150), then transferred by a fluid connecting element (140) to the inlets of one or several nanofluidic biosensors (120). The capsule system (100) is disposed on an external support (200), and finally an optical or an electrical measurement unit (500) is used to measure the molecular interactions in the nanofluidic biosensors.

Abstract: A nanofluidic biosensor system (200) comprising a bottom substrate (120) and a top substrate (110) between which are defined an input lateral aperture (210), a nanoslit (230) which contains at least one functionalized area (231) and an output lateral aperture (220) or an internal reservoir (221), said biosensor system (200) being adapted to let a solution containing biomolecules (320) enter the input lateral aperture (210) and successively pass through said nanoslit (230) and said output lateral aperture (220) or internal reservoir (221); said biosensor system (200) furthermore comprising a gas evacuation subsystem (150-155) which is located between said nanoslit (230) and the biosensor external environment.

Abstract: A method and device for multiplexing and calibrating rapid quantification of biomolecules present in a nanofluidic biosensor composed by a nanoslit. A novel concept defining multiple different local structured areas containing biomarkers. Local structured areas can also be structured to decrease the biomarkers density in the nanoslit. Such enables the multiplexed quantification biomolecular interactions of interest in the same nanofluidic biosensor.

Abstract: A method and device for the rapid quantification of biomolecules (320) present in a nanochannel (210) is claimed. In particular, the present invention relates to a novel concept of liquid actuation and selectively functionalized surfaces in a nanochannel that create a concentration gradient of transitory immobilized biomolecules (340) across the nanochannel. The present concept enables the quantification of biomolecular interactions of interest (320).

Abstract: A method and device for the rapid quantification of biomolecules (320) present in a nanochannel (210) is claimed. In particular, the present invention relates to a novel concept of liquid actuation and selectively functionalized surfaces in a nanochannel that create a concentration gradient of transitory immobilized biomolecules (340) across the nanochannel. The present concept enables the quantification of biomolecular interactions of interest (320).