Patents Assigned to ABSCI, LLC
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Patent number: 11584785Abstract: A connecting polypeptide has SEQ ID NO:73. A proinsulin polypeptide includes a mature insulin A-chain, a mature insulin B-chain, and a connecting peptide comprising SEQ ID NO: 73 linking the mature A-chain and the mature B-chain, wherein the connecting peptide is not a native human proinsulin C-peptide. The proinsulin polypeptides according to the invention can be made in high titers and in high purity.Type: GrantFiled: May 4, 2020Date of Patent: February 21, 2023Assignee: ABSCI, LLCInventors: Amy Doerner, Michael Cammarata, Tyler Nusca, Suzanne A. Sprunger, Melissa Patterson
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Patent number: 11447781Abstract: The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.Type: GrantFiled: February 13, 2020Date of Patent: September 20, 2022Assignee: ABSCI LLCInventors: Sean McClain, Mark Valasek
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Patent number: 11371048Abstract: The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.Type: GrantFiled: September 11, 2016Date of Patent: June 28, 2022Assignee: ABSCI LLCInventors: Sean McClain, Mark Valasek, Philip Barish, Jeremy Minshull
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Publication number: 20200299343Abstract: A connecting polypeptide has SEQ ID NO:73. A proinsulin polypeptide includes a mature insulin A-chain, a mature insulin B-chain, and a connecting peptide comprising SEQ ID NO: 73 linking the mature A-chain and the mature B-chain, wherein the connecting peptide is not a native human proinsulin C-peptide. The proinsulin polypeptides according to the invention can be made in high titers and in high purity.Type: ApplicationFiled: May 4, 2020Publication date: September 24, 2020Applicant: AbSci LLCInventors: Amy Doerner, Michael Cammarata, Fred Larimore, Tyler Nusca, Suzanne A. Sprunger, Melissa Patterson
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Publication number: 20200270338Abstract: A cell for producing insulin includes an E. coli host cell and a polynucleotide designed for production of insulin disposed within the E. coli host cell. The polynucleotide includes a first inducible promoter and a first coding sequence to be transcribed from the first inducible promoter, the first coding sequence encoding an insulin polypeptide. The polynucleotide also includes a second inducible promoter and a second coding sequence to be transcribed from the second inducible promoter, the second coding sequence encoding one or more of cDsbA, cDsbC, a protein disulfide isomerase, Ervlp, and a chaperone. The first inducible promoter and the second inducible promoter are not responsive to the same inducer. A method of producing insulin includes growing a sample of the cells in a fermentation volume between 0.1 L and 1,000,000 L, adding an inducer of the first inducible promoter to induce expression of the insulin polypeptide, and purifying the insulin polypeptide.Type: ApplicationFiled: May 11, 2020Publication date: August 27, 2020Applicant: AbSci LLCInventors: Sean McClain, Philip Barish
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Publication number: 20200172915Abstract: The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.Type: ApplicationFiled: February 13, 2020Publication date: June 4, 2020Applicant: AbSci LLCInventors: Sean McClain, Mark Valasek
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Patent number: 10604761Abstract: The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.Type: GrantFiled: February 16, 2017Date of Patent: March 31, 2020Assignee: AbSci LLCInventors: Sean McClain, Mark Valasek
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Publication number: 20200032276Abstract: The present invention is an inducible coexpression system, capable of controlled induction of expression of each gene product.Type: ApplicationFiled: October 7, 2019Publication date: January 30, 2020Applicant: AbSci, LLCInventors: Sean McClain, Mark Valasek
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Patent number: 10508270Abstract: The present invention provides methods of producing thrombin using coordinated coexpression systems, and particularly inducible coexpression systems, capable of controlled induction of expression of each gene product required for the production of thrombin, and the thrombin produced by this method.Type: GrantFiled: February 5, 2014Date of Patent: December 17, 2019Assignees: AbSci LLC, Aronora Inc.Inventors: Sean McClain, Mark Valasek, Andras Gruber
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Publication number: 20180282405Abstract: The present invention provides host cells having an oxidizing cytoplasm, which are capable of growing to high cell densities and producing recombinant proteins in soluble form. Also provided are methods for growing such host cells to high densities, methods for optionally modulating the growth rates of the host cells, and methods for inducing the host cells to produce the desired gene product in soluble form. Further aspects of the invention relate to host cell preparation and storage methods, which utilize the advantages of producing gene products in host cell cytoplasm to provide methods for long-term storage and/or transport of expressed gene products that are retained in a stable and soluble form within the host cells.Type: ApplicationFiled: June 14, 2018Publication date: October 4, 2018Applicant: AbSci, LLCInventors: Sean McClain, Philip Barish
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Publication number: 20170159061Abstract: The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.Type: ApplicationFiled: February 16, 2017Publication date: June 8, 2017Applicant: AbSci, LLCInventors: Sean McClain, Mark Valasek
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Patent number: 9617335Abstract: The present invention is an inducible coexpression system, capable of controlled induction of expression of each gene product.Type: GrantFiled: August 5, 2013Date of Patent: April 11, 2017Assignee: AbSci LLCInventors: Sean McClain, Mark Valasek
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Publication number: 20160376602Abstract: The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.Type: ApplicationFiled: September 11, 2016Publication date: December 29, 2016Applicant: AbSci, LLCInventors: Sean McClain, Mark Valasek, Philip Barish, Jeremy Minshull
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Publication number: 20160152701Abstract: The present invention is an inducible coexpression system, capable of controlled induction of expression of each gene product.Type: ApplicationFiled: November 25, 2015Publication date: June 2, 2016Applicant: ABSCI, LLCInventors: Sean McClain, Mark Valasek
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Publication number: 20150353940Abstract: The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.Type: ApplicationFiled: June 16, 2015Publication date: December 10, 2015Applicant: ABSCI, LLCInventors: Sean McClain, Mark Valasek, Philip Barish, Jeremy Minshull