Patents Assigned to Access Medical Systems, Ltd.
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Publication number: 20150276724Abstract: The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.Type: ApplicationFiled: January 27, 2014Publication date: October 1, 2015Applicant: Access Medical Systems, LTD.Inventors: Robert F. ZUK, Hong Tan
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Patent number: 8940238Abstract: The present invention is directed to an assembly for use in detecting an analyte in a sample based on thin-film spectral interference. The assembly comprises a waveguide, a monolithic substrate optically coupled to the waveguide, and a thin-film layer directly bonded to the sensing side of the monolithic substrate. The refractive index of the monolithic substrate is higher than the refractive index of the transparent material of the thin-film layer. A spectral interference between the light reflected into the waveguide from a first reflecting surface and a second reflecting surface varies as analyte molecules in a sample bind to the analyte binding molecules coated on the thin-film layer.Type: GrantFiled: December 3, 2013Date of Patent: January 27, 2015Assignee: Access Medical Systems, Ltd.Inventors: Hong Tan, Yushan Tan, Erhua Cao, Ming Xia, Robert F. Zuk
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Patent number: 8753574Abstract: This invention relates to a cartridge for an immunoassay test. The cartridge comprises (a) a probe well comprising a probe and a cap, the cap being in a closed position to enclose the probe in the probe well, wherein the probe has a bottom tip coated with analyte-binding molecules; (b) a sample well to receive a sample; (c) one or more reagent wells; (d) a plurality of wash wells each containing a first aqueous solution; and (e) a measurement well having a light transmissive bottom, the measurement well containing a second aqueous solution; wherein the openings of the sample well, reagent well, measurement well and wash wells are sealed. The present invention also relates to an apparatus for loading and releasing a probe. The apparatus comprises a push pin and a groove to load and transfer the probe to a plurality of locations, such as different wells in the above-mentioned cartridge, to conduct the immunoassay test.Type: GrantFiled: July 5, 2013Date of Patent: June 17, 2014Assignee: Access Medical Systems, Ltd.Inventors: Hong Tan, Ming Xia, Yushan Tan, Jun Chen, Erhua Cao, Genqian Li, Robert F. Zuk
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Publication number: 20140128596Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.Type: ApplicationFiled: March 18, 2013Publication date: May 8, 2014Applicant: ACCESS MEDICAL SYSTEMS, LTD.Inventor: Robert F. ZUK
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Patent number: 8647889Abstract: The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.Type: GrantFiled: November 14, 2012Date of Patent: February 11, 2014Assignee: Access Medical Systems, Ltd.Inventors: Robert F. Zuk, Hong Tan
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Patent number: 8597578Abstract: The present invention is directed to an assembly for use in detecting an analyte in a sample based on thin-film spectral interference. The assembly comprises a waveguide, a monolithic substrate optically coupled to the waveguide, and a thin-film layer directly bonded to the sensing side of the monolithic substrate. The refractive index of the monolithic substrate is higher than the refractive index of the transparent material of the thin-film layer. A spectral interference between the light reflected into the waveguide from a first reflecting surface and a second reflecting surface varies as analyte molecules in a sample bind to the analyte binding molecules coated on the thin-film layer.Type: GrantFiled: August 19, 2011Date of Patent: December 3, 2013Assignee: Access Medical Systems, Ltd.Inventors: Hong Tan, Yushan Tan, Erhua Cao, Min Xia, Robert F. Zuk
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Publication number: 20130273667Abstract: The present invention relates to a method for quantitating an analyte having a wide range concentration in a single assay without having to dilute the sample and repeating the assay. The key feature of the invention is having two cycles of events including sample binding to probe, binding reactions, and detection. After the first cycle of binding and detecting, the probe is dipped into the same sample vessel to bind additional analyte in the sample vessel at a condition that is more favorable to binding than the condition in the first cycle.Type: ApplicationFiled: March 11, 2013Publication date: October 17, 2013Applicant: ACCESS MEDICAL SYSTEMS, LTD.Inventor: Robert F. ZUK
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Patent number: 8501496Abstract: The present invention is directed immunoassay cuvettes that comprise diffusely bound and non-diffusely bound reagents for carrying out an immunoassay. The reaction and detection are carried out in the immunoassay cuvette. The immunoassay cuvette comprises a transparent front wall, a back wall, side walls, a bottom, and a top opening. The back wall of the cuvette has a substantially planar surface made of a non-porous material and comprises a capture zone having reagents non-diffusedly bound and a signal reagent zone having reagents diffusedly bound.Type: GrantFiled: October 17, 2012Date of Patent: August 6, 2013Assignee: Access Medical Systems, Ltd.Inventors: Robert F. Zuk, Hong Tan
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Patent number: 8492139Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.Type: GrantFiled: October 24, 2012Date of Patent: July 23, 2013Assignee: Access Medical Systems, Ltd.Inventors: Hong Tan, Robert F. Zuk, Yushan Tan, Erhua Cao, Min Xia, Jun Chen
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Patent number: 8415172Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.Type: GrantFiled: October 16, 2012Date of Patent: April 9, 2013Assignee: Access Medical Systems, Ltd.Inventor: Robert F. Zuk
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Publication number: 20130071952Abstract: The present invention is directed to a luminescent immunoassay method for detecting an analyte in a liquid sample with high sensitivity. The invention provides a unique combination of (i) using a probe having a small sensing surface area for binding analyte molecules, (ii) using a high molecular weight branched polymer conjugated with multiple binding molecules and multiple luminescent labels, and (iii) cycling the probe having immunocomplex formed back to the reagent vessel and amplification vessel 1-10 times and repeating the reaction with the reagent and the amplification polymer, to improve the sensitivity of detection level. For each cycling, the luminescent signal is increased significantly over the noise.Type: ApplicationFiled: November 14, 2012Publication date: March 21, 2013Applicant: ACCESS MEDICAL SYSTEMS, LTD.Inventor: Access Medical Systems, LTD.
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Publication number: 20130052081Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.Type: ApplicationFiled: October 24, 2012Publication date: February 28, 2013Applicant: ACCESS MEDICAL SYSTEMS, LTD.Inventor: Access Medical Systems, LTD.
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Publication number: 20130040378Abstract: The present invention is directed immunoassay cuvettes that comprise diffusely bound and non-diffusely bound reagents for carrying out an immunoassay. The reaction and detection are carried out in the immunoassay cuvette. The immunoassay cuvette comprises a transparent front wall, a back wall, side walls, a bottom, and a top opening. The back wall of the cuvette has a substantially planar surface made of a non-porous material and comprises a capture zone having reagents non-diffusedly bound and a signal reagent zone having reagents diffusedly bound.Type: ApplicationFiled: October 17, 2012Publication date: February 14, 2013Applicant: ACCESS MEDICAL SYSTEMS, LTD.Inventor: Access Medical Systems, LTD.
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Publication number: 20130041141Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked FICOLL® molecule having a plurality of binding molecules and a plurality of fluorescent labels.Type: ApplicationFiled: October 16, 2012Publication date: February 14, 2013Applicant: ACCESS MEDICAL SYSTEMS, LTD.Inventor: ACCESS MEDICAL SYSTEMS, LTD.
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Patent number: 8309369Abstract: This invention relates to a detection system for measuring a fluorescent signal in a fluorescent assay. The system comprises a probe having a small sensing surface bound with a fluorescent label, and a light source and a detector both mounted at the proximal side of the sensing surface of the substrate. The invention also relates to a method for detecting an analyte in a liquid sample using a probe tip having a small surface area (?5 mm) and a high molecular weight polymer (?1 MD) having multiple binding molecules and multiple fluorescent labels. The binding reaction is accelerated by flowing the reaction solutions laterally and moving the probe tip up and down in the reaction vessels. The invention furthers relates to a fluorescent labeling composition comprising a cross-linked Ficoll molecule having a plurality of binding molecules and a plurality of fluorescent labels.Type: GrantFiled: September 1, 2011Date of Patent: November 13, 2012Assignee: Access Medical Systems, Ltd.Inventors: Hong Tan, Robert F. Zuk, Yushan Tan, Erhua Cao, Min Xia, Jun Chen
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Patent number: 8304255Abstract: The present invention is directed immunoassay cuvettes that comprise diffusely bound and non-diffusely bound reagents for carrying out an immunoassay. The reaction and detection are carried out in the immunoassay cuvette. The immunoassay cuvette comprises a transparent front wall, a back wall, side walls, a bottom, and a top opening. The back wall of the cuvette has a substantially planar surface made of a non-porous material and comprises a capture zone having reagents non-diffusedly bound and a signal reagent zone having reagents diffusedly bound.Type: GrantFiled: February 11, 2009Date of Patent: November 6, 2012Assignee: Access Medical Systems, Ltd.Inventors: Robert F. Zuk, Hong Tan
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Patent number: D737993Type: GrantFiled: August 13, 2013Date of Patent: September 1, 2015Assignee: ACCESS MEDICAL SYSTEMS, LTD.Inventors: Hong Tan, Genqian Li, Ming Xia, Yongli Ren