Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.
Type:
Grant
Filed:
December 6, 2018
Date of Patent:
August 2, 2022
Assignee:
Advanced Enzyme Systems, LLC
Inventors:
Robert Muko Tjon-Joe-Pin, Moreland David Gibbs, Valentino Setoa Junior Te'o
Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.
Type:
Application
Filed:
December 6, 2018
Publication date:
March 28, 2019
Applicant:
Advanced Enzyme Systems, LLC
Inventors:
Robert Muko Tjon-Joe-Pin, Moreland David Gibbs, Valentino Setoa Junior Te'o
Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.
Type:
Grant
Filed:
November 25, 2014
Date of Patent:
March 12, 2019
Assignee:
Advanced Enzyme Systems, LLC
Inventors:
Robert Muko Tjon-Joe-Pin, Moreland David Gibbs, Valentino Setoa Junior Te'o
Abstract: Novel hyperthermophilic Dictyoglomus beta-mannanases are provided for use in high temperature industrial applications requiring enzymatic hydrolysis of 1,4-?-D-mannosidic linkages in mannans, galactomannans, and glucomannans. Also provided are methods and compositions for fracturing a subterranean formation in which a gellable fracturing fluid is first formed by blending together a hydratable polymer and a Dictyoglomus beta-mannanase as an enzyme breaker. An optimized and stabilized recombinant Dictyoglomus beta-mannanase is provided that shows superior performance/effectiveness and properties in degrading guar and derivatized guars at pH ranges from 3.0 to 12 and temperatures ranging from 130° F. to in excess of 270° F.
Type:
Application
Filed:
November 25, 2014
Publication date:
May 28, 2015
Applicant:
ADVANCED ENZYME SYSTEMS, LLC
Inventors:
Robert Muko Tjon-Joe-Pin, Moreland David Gibbs, Valentino Setoa Junior Te'o