Abstract: Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.
Type:
Application
Filed:
November 19, 2004
Publication date:
July 21, 2005
Applicants:
Affymetrix, Inc., Affymax Technologies, N.V., a Netherlands Antilles corporation
Inventors:
David Lockhart, Dirk Vetter, Martin Diggelmann
Abstract: A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.
Type:
Application
Filed:
November 24, 2004
Publication date:
June 9, 2005
Applicants:
Affymetrix, Inc., AFFYMAX TECHNOLOGIES N.V., a Netherlands Antilles corporation
Inventors:
James Winkler, Stephen Fodor, Christopher Buchko, Debra Ross, Lois Aldwin, Douglas Modlin
Abstract: A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
Type:
Application
Filed:
September 30, 2002
Publication date:
May 1, 2003
Applicant:
AFFYMAX TECHNOLOGIES, N.V., a Netherlands Antilles corporation
Inventors:
Stephen P.A. Fodor, Lubert Stryer, James L. Winkler, Christopher P. Holmes, Dennis W. Solas