Patents Assigned to Agena BioScience, Inc.
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Patent number: 11827928Abstract: Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.Type: GrantFiled: August 6, 2021Date of Patent: November 28, 2023Assignee: AGENA BIOSCIENCE, INC.Inventors: Martin Beaulieu, Dirk Johannes Van den Boom
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Patent number: 11680289Abstract: Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species.Type: GrantFiled: November 16, 2020Date of Patent: June 20, 2023Assignee: AGENA BIOSCIENCE, INC.Inventor: Anders Olof Herman Nygren
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Patent number: 11667958Abstract: Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.Type: GrantFiled: February 18, 2020Date of Patent: June 6, 2023Assignee: AGENA BIOSCIENCE, INC.Inventors: Christiane Honisch, Dirk Van Den Boom, Michael Mosko, Anders Nygren
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Patent number: 11111530Abstract: Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.Type: GrantFiled: January 5, 2018Date of Patent: September 7, 2021Assignee: Agena Biosciences, Inc.Inventors: Martin Beaulieu, Dirk Johannes Van Den Boom
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Patent number: 10865439Abstract: Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species.Type: GrantFiled: October 30, 2019Date of Patent: December 15, 2020Assignee: Agena Bioscience, Inc.Inventor: Anders Olof Herman Nygren
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Patent number: 10774375Abstract: Provided herein are products and processes for the quantitation of mitochondrial nucleic acid in a sample from a subject. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and genomic polynucleotides from nucleic acid for a sample under amplification conditions. In certain aspects are multiplex methods for determining dosage of mitochondrial nucleic acid relative to genomic nucleic acid for a sample from a subject including amplifying sets of mitochondrial polynucleotides and amplifying sets of nuclear polynucleotides from nucleic acid for a sample under amplification conditions.Type: GrantFiled: September 16, 2016Date of Patent: September 15, 2020Assignee: Agena Bioscience, Inc.Inventor: Anders Olof Herman Nygren
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Patent number: 10640817Abstract: Provided herein are multiplex methods for detecting the presence or absence and amount of variants of a plurality of target nucleic acid species having low-abundance variants and high-abundance variants.Type: GrantFiled: April 22, 2016Date of Patent: May 5, 2020Assignee: Agena Bioscience, Inc.Inventor: Anders Olof Herman Nygren
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Patent number: 10604791Abstract: Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.Type: GrantFiled: July 17, 2012Date of Patent: March 31, 2020Assignee: Agena Bioscience, Inc.Inventors: Christiane Honisch, Dirk Johannes Van Den Boom, Michael Mosko, Anders Nygren
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Patent number: 10513728Abstract: Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species.Type: GrantFiled: January 23, 2019Date of Patent: December 24, 2019Assignee: Agena BioScience, Inc.Inventor: Anders Olof Herman Nygren
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Patent number: 10329612Abstract: The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.Type: GrantFiled: December 13, 2017Date of Patent: June 25, 2019Assignee: Agena Bioscience, Inc.Inventor: Thomas Becker
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Patent number: 10233489Abstract: Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species.Type: GrantFiled: April 22, 2016Date of Patent: March 19, 2019Assignee: Agena Bioscience, Inc.Inventor: Anders Olof Herman Nygren
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Patent number: 10204771Abstract: Provided herein are compositions and methods useful for preparing and analyzing a sample on a substrate by matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). In some embodiments, compositions provided herein comprise a substrate, matrix and nanoparticles, and sometimes comprise one or more additives and sometimes an analyte. Compositions provided herein sometimes comprise nanoparticles that include or are made up of silicon dioxide.Type: GrantFiled: March 7, 2016Date of Patent: February 12, 2019Assignee: Agena Bioscience, Inc.Inventors: Thomas Becker, Stefan Berkenkamp
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Patent number: 9896724Abstract: Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.Type: GrantFiled: May 22, 2015Date of Patent: February 20, 2018Assignee: Agena Bioscience, Inc.Inventors: Martin Beaulieu, Dirk Johannes van den Boom
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Patent number: 9873912Abstract: The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.Type: GrantFiled: March 9, 2016Date of Patent: January 23, 2018Assignee: Agena Bioscience, Inc.Inventor: Thomas Becker
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Patent number: 9669376Abstract: A substrate for use in mass spectrometric analyzes having an array of target locations.Type: GrantFiled: March 12, 2015Date of Patent: June 6, 2017Assignee: AGENA BIOSCIENCE, INC.Inventors: Thomas Becker, Aaron A. Hanson
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Patent number: 9394565Abstract: Fragmentation-based methods and systems, particularly mass spectrometry based methods and systems, for the analysis of sequence variations including haplotypes are provided. Also provided are methods for obtaining a specific allele from a nucleic acid mixture, such as genomic DNA.Type: GrantFiled: September 2, 2004Date of Patent: July 19, 2016Assignee: AGENA BIOSCIENCE, INC.Inventors: Martin Beaulieu, Dirk J. Van Den Boom
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Patent number: 9310378Abstract: The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.Type: GrantFiled: January 7, 2011Date of Patent: April 12, 2016Assignee: Agena Bioscience, Inc.Inventor: Thomas Becker
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Patent number: 9305756Abstract: Provided herein are compositions and methods useful for preparing and analyzing a sample on a substrate by matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). In some embodiments, compositions provided herein comprise a substrate, matrix and nanoparticles, and sometimes comprise one or more additives and sometimes an analyte. Compositions provided herein sometimes comprise nanoparticles that include or are made up of silicon dioxide.Type: GrantFiled: March 13, 2013Date of Patent: April 5, 2016Assignee: Agena Bioscience, Inc.Inventors: Thomas Becker, Stefan Berkenkamp
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Patent number: 9249456Abstract: Methods, combinations and kits are provided for identifying the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or for identifying the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule. Methylation state identification is performed by treating a methylated target nucleic acid molecule with a reagent that modifies one or more nucleotides in the target nucleic acid molecule as a function of the methylation state of the target nucleic acid molecule, methylation specifically amplifying treated target nucleic acid molecule, fragmenting amplified products, and detecting one or more fragments to thereby identify the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule.Type: GrantFiled: March 24, 2005Date of Patent: February 2, 2016Assignee: AGENA BIOSCIENCE, INC.Inventors: Mathias Ehrich, Dirk Johannes Van Den Boom
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Patent number: 9068953Abstract: Embodiments include integrated robotic sample transfer devices and components thereof which are used for reliably and accurately transferring small samples of material from one registered position to another registered position. Such transfers of material may be carried out by a single pin tool or an array of pin tools of a pin tool head assembly of robotic sample transfer devices. Some embodiments also include automated cleaning of the pin tools used to transfer the sample material. Some embodiments are fully integrated units having internal fluid supply and waste tanks, vacuum source, fluid pumps, controllers and user interface devices.Type: GrantFiled: September 24, 2012Date of Patent: June 30, 2015Assignee: Agena Bioscience, Inc.Inventors: Rolf Silbert, Richard Capella, Justin Cuzens