Patents Assigned to Ahram Biosystems Inc.
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Publication number: 20190210028Abstract: Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: ApplicationFiled: August 22, 2018Publication date: July 11, 2019Applicant: Ahram Biosystems, Inc.Inventor: Hyun Jin Hwang
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Publication number: 20190168215Abstract: Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: ApplicationFiled: August 22, 2018Publication date: June 6, 2019Applicant: Ahram Biosystems, Inc.Inventor: Hyun Jin Hwang
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Patent number: 10086375Abstract: Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: GrantFiled: January 4, 2017Date of Patent: October 2, 2018Assignee: Ahram Biosystems, Inc.Inventor: Hyun Jin Hwang
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Patent number: 10086374Abstract: Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: GrantFiled: January 4, 2017Date of Patent: October 2, 2018Assignee: Ahram Biosystems, Inc.Inventor: Hyun Jin Hwang
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Patent number: 9765376Abstract: The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.Type: GrantFiled: October 17, 2013Date of Patent: September 19, 2017Assignee: AHRAM BIOSYSTEMS, INC.Inventors: Hyun Jin Hwang, Jeong Hee Kim, Kyunghoon Jeong
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Publication number: 20170239662Abstract: Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: ApplicationFiled: January 4, 2017Publication date: August 24, 2017Applicant: Ahram Biosystems, Inc.Inventor: Hyun Jin HWANG
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Publication number: 20170239654Abstract: Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: ApplicationFiled: January 4, 2017Publication date: August 24, 2017Applicant: Ahram Biosystems, Inc.Inventor: Hyun Jin HWANG
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Patent number: 9573133Abstract: Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: GrantFiled: July 2, 2012Date of Patent: February 21, 2017Assignee: AHRAM BIOSYSTEMS, INC.Inventor: Hyun Jin Hwang
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Patent number: 9573134Abstract: Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: GrantFiled: July 2, 2012Date of Patent: February 21, 2017Assignee: AHRAM BIOSYSTEMS, INC.Inventor: Hyun Jin Hwang
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Patent number: 9346055Abstract: The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.Type: GrantFiled: January 9, 2009Date of Patent: May 24, 2016Assignee: AHRAM BIOSYSTEMS, INC.Inventors: Hyun Jin Hwang, Jeong Hee Kim, Kyunghoon Jeong
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Publication number: 20140170707Abstract: The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.Type: ApplicationFiled: October 17, 2013Publication date: June 19, 2014Applicant: AHRAM BIOSYSTEMS, INC.Inventors: Hyun Jin HWANG, Jeong Hee KIM, Kyunghoon JEONG
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Publication number: 20130109021Abstract: Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: ApplicationFiled: July 2, 2012Publication date: May 2, 2013Applicant: AHRAM BIOSYSTEMS, INC.Inventor: Hyun Jin Hwang
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Publication number: 20130109022Abstract: Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.Type: ApplicationFiled: July 2, 2012Publication date: May 2, 2013Applicant: AHRAM BIOSYSTEMS, INC.Inventor: HYUN JIN HWANG
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Publication number: 20120021463Abstract: The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.Type: ApplicationFiled: October 3, 2011Publication date: January 26, 2012Applicant: Ahram Biosystems, Inc.Inventors: Hyun Jin Hwang, Jeong Hee Kim, Kyunghoon Jeong
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Patent number: 8067174Abstract: The present invention relates to a DNA polymerase immobilized by covalent bonding. More particularly, the present invention relates to an immobilized DNA polymerase whose activity is maximally preserved by masking the active site of the DNA polymerase and optimizing interaction of the masked molecule to the substrate material. In one embodiment, the average activity of the immobilized DNA polymerase is more than about 10% relative to that of the solution phase DNA polymerase. Further provided by the invention are methods and kits for performing polymerase chain reactions (PCR).Type: GrantFiled: May 30, 2007Date of Patent: November 29, 2011Assignee: Ahram Biosystems Inc.Inventors: Hyun Jin Hwang, Jeong Hee Kim
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Patent number: 8053215Abstract: The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply heat to, or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.Type: GrantFiled: October 30, 2009Date of Patent: November 8, 2011Assignee: Ahram Biosystems, Inc.Inventors: Hyun Jin Hwang, Jeong Hee Kim, Kyunghoon Jeong
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Patent number: 7855072Abstract: Disclosed is a system for detecting a protease inside a cell. In one embodiment, the system includes a chimeric protein that comprises as covalently linked components: 1) at least one optionally masked signal protein; 2) at least one protease-specific cleavage site; and 3) at least one detectable amino acid sequence. The invention has a wide spectrum of applications including use in the detection of novel protease inhibitors inside cells and tissue.Type: GrantFiled: August 16, 2006Date of Patent: December 21, 2010Assignee: Ahram Biosystems Inc.Inventors: Inhwan Hwang, Dae Heon Kim, Yong Jik Lee
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Publication number: 20100285536Abstract: The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.Type: ApplicationFiled: October 30, 2009Publication date: November 11, 2010Applicant: Ahram Biosystems, Inc.Inventors: Hyun Jin Hwang, Jeong Hee Kim, Kyunghoon Jeong
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Patent number: 7628961Abstract: The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply heat to, or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.Type: GrantFiled: March 15, 2004Date of Patent: December 8, 2009Assignee: Ahram Biosystems, Inc.Inventors: Hyun Jin Hwang, Jeong Hee Kim, Kyunghoon Jeong
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Patent number: 7488595Abstract: The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.Type: GrantFiled: April 29, 2004Date of Patent: February 10, 2009Assignee: Ahram Biosystems Inc.Inventors: Hyun Jin Hwang, Jeong Hee Kim, Kyunghoon Jeong