Patents Assigned to Akonni Biosystems, Inc.
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Patent number: 11767549Abstract: An integrated sample purification system includes a housing, a sample container rack, a filter holder, and a cylindrical magnet. The sample container rack and the filter device holder are disposed in the housing. The sample container rack is configured to hold one or more sample containers, the filter device holder is configured to hold one or more filter devices. The cylindrical magnet is adjacent to and external to the sample container rack, and is rotated about a central, longitudinal axis of the magnet by an electric motor disposed in the housing to lyse cells. Molecules of interest in the lysed cells are purified using filters that bind specifically to the molecules of interest. The system is readily amenable to automation and rapid purification and analysis of molecules of interest, such as nucleic acids and proteins.Type: GrantFiled: December 30, 2020Date of Patent: September 26, 2023Assignee: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. Cooney, Rebecca Holmberg, Phillip Belgrader, Peter Qiang Qu
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Patent number: 11747332Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.Type: GrantFiled: November 24, 2020Date of Patent: September 5, 2023Assignee: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. Cooney, Peter Qiang Qu, Alexander Perov, Jennifer Parker
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Patent number: 11097278Abstract: A molecular testing device comprises a heating and cooling module having a thin-film thermoelectric heating and cooling device, and a removable test module having a combined amplification and hybridization reaction chamber. The reaction chamber comprises a thermo-conductive exterior surface and a microarray on an interior surface. The thin-film thermoelectric heating and cooling device has a heat transfer surface that is adapted to make contact with the thermo-conductive exterior surface of the reaction chamber. The molecular testing device may be used to perform a PCR in the reaction chamber.Type: GrantFiled: September 24, 2018Date of Patent: August 24, 2021Assignee: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. Cooney, Peter Qiang Qu, Arial Bueno
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Patent number: 10882046Abstract: A molecular testing device comprises a heating and cooling module having a thin-film thermoelectric heating and cooling device, and a removable test module having a combined amplification and hybridization reaction chamber. The reaction chamber comprises a thermo-conductive exterior surface and a microarray on an interior surface. The thin-film thermoelectric heating and cooling device has a heat transfer surface that is adapted to make contact with the thermo-conductive exterior surface of the reaction chamber. The molecular testing device may be used to perform a PCR in the reaction chamber.Type: GrantFiled: August 21, 2018Date of Patent: January 5, 2021Assignee: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. Cooney, Peter Qiang Qu, Arial Bueno
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Patent number: 10877028Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.Type: GrantFiled: September 13, 2019Date of Patent: December 29, 2020Assignee: Akonni Biosystems, Inc.Inventors: Christopher G. Cooney, Peter Qiang Qu, Alexander Perov, Jennifer Parker
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Patent number: 10532352Abstract: An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation/analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.Type: GrantFiled: April 3, 2019Date of Patent: January 14, 2020Assignee: Akonni Biosystems, Inc.Inventors: Christopher G. Cooney, Phillip Belgrader, Arial Bueno, Steve Garber, Nitu Harshendu Thakore, Peter Qiang Qu
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Patent number: 10300482Abstract: An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation/analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.Type: GrantFiled: December 8, 2011Date of Patent: May 28, 2019Assignee: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. Cooney, Phillip Belgrader, Arial Bueno, Steve Garber, Nitu Harshendu Thakore, Peter Qiang Qu
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Patent number: 10138458Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.Type: GrantFiled: December 22, 2015Date of Patent: November 27, 2018Assignee: Akonni Biosystems, Inc.Inventors: Phillip Belgrader, Benjamin Hindson
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Patent number: 10125388Abstract: An integrated sample purification system includes a housing, a sample container rack, a filter holder, and a cylindrical magnet. The sample container rack and the filter device holder are disposed in the housing. The sample container rack is configured to hold one or more sample containers, the filter device holder is configured to hold one or more filter devices. The cylindrical magnet is adjacent to and external to the sample container rack, and is rotated about a central, longitudinal axis of the magnet by an electric motor disposed in the housing to lyse cells. Molecules of interest in the lysed cells are purified using filters that bind specifically to the molecules of interest. The system is readily amenable to automation and rapid purification and analysis of molecules of interest, such as nucleic acids and proteins.Type: GrantFiled: December 1, 2015Date of Patent: November 13, 2018Assignee: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. Cooney, Rebecca Holmberg, Phillip Belgrader, Peter Qiang Qu
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Patent number: 10093919Abstract: Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter.Type: GrantFiled: July 20, 2016Date of Patent: October 9, 2018Assignee: Akonni Biosystems, Inc.Inventors: Rebecca Holmberg, Alissa Erin Burkey, Tinsley Janna Stokes, Phillip Belgrader
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Patent number: 10081016Abstract: A molecular testing device comprises a heating and cooling module having a thin-film thermoelectric heating and cooling device, and a removable test module having a combined amplification and hybridization reaction chamber. The reaction chamber comprises a thermo-conductive exterior surface and a microarray on an interior surface. The thin-film thermoelectric heating and cooling device has a heat transfer surface that is adapted to make contact with the thermo-conductive exterior surface of the reaction chamber. The molecular testing device may be used to perform a PCR in the reaction chamber.Type: GrantFiled: June 18, 2015Date of Patent: September 25, 2018Assignee: Akonni Biosystems, Inc.Inventors: Christopher G. Cooney, Peter Qiang Qu, Arial Bueno
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Patent number: 9753047Abstract: A positioning system for a sample analysis device is disclosed. The positioning system comprises (1) a carousel comprising a platform and a sample loading tray mounted on the platform, and (2) a stage comprising a positioning system for positioning the carousel under the optical path of an imaging system. The sample loading tray is configured for holding a cartridge comprising one or more lateral flow cells (LFCs).Type: GrantFiled: October 26, 2015Date of Patent: September 5, 2017Assignee: AKONNI BIOSYSTEMS INC.Inventors: Christopher Cooney, Alexander Perov, Arial Bueno, Charles Daitch
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Patent number: 9493815Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.Type: GrantFiled: June 3, 2014Date of Patent: November 15, 2016Assignee: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. Cooney, Peter Qiang Qu, Alexander Perov, Jennifer Parker
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Patent number: 9428746Abstract: Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter.Type: GrantFiled: August 27, 2013Date of Patent: August 30, 2016Assignee: Akonni Biosystems, Inc.Inventors: Rebecca Holmberg, Alissa Erin Gindlesperger, Tinsley Janna Stokes, Phillip Belgrader
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Patent number: 9217174Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.Type: GrantFiled: February 12, 2013Date of Patent: December 22, 2015Assignees: Akonni Biosystems, Inc., Bio-Rad LaboratoriesInventors: Phillip Belgrader, Benjamin Hindson
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Patent number: 9074245Abstract: A microarray-based sample analysis (MBSA) system includes a cartridge holder adapted to receive a replaceable cartridge that is configured to receive a detachable, replaceable sample analysis unit containing one or more reaction chambers for sample analysis; a fluid control subsystem that controls fluid flow; and an optical subsystem configured to capture an image of the microarray.Type: GrantFiled: July 5, 2012Date of Patent: July 7, 2015Assignee: AKONNI BIOSYSTEMS, INCInventors: Phillip Belgrader, Christopher G. Cooney, Aleksandr N. Perov
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Patent number: 8906652Abstract: Methods and devices for performing chemical reactions under controlled temperature are described. In one embodiment, the devices provided by the invention comprise a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing. The reaction chamber has thermally conductive interior and exterior surfaces defining an internal volume therein at a first temperature. The device also includes at least one temperature-control substance at a second temperature received into said bladder and expels said temperature-control substance, the bladder expands to about substantially at least a portion of said exterior surfaces of said reaction chamber to enable thermal exchange between said temperature-control substance and the said internal volume of reaction chamber.Type: GrantFiled: August 1, 2012Date of Patent: December 9, 2014Assignee: Akonni Biosystems, Inc.Inventor: Phillip Belgrader
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Publication number: 20140287954Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.Type: ApplicationFiled: June 3, 2014Publication date: September 25, 2014Applicant: AKONNI BIOSYSTEMS, INC.Inventors: Christopher G. COONEY, Peter Qiang QU, Alexander PEROV, Jennifer PARKER
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Patent number: 8828912Abstract: A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.Type: GrantFiled: April 13, 2012Date of Patent: September 9, 2014Assignee: Akonni Biosystems, Inc.Inventor: Christopher G. Cooney
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Patent number: 8759085Abstract: A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.Type: GrantFiled: April 29, 2011Date of Patent: June 24, 2014Assignee: Akonni Biosystems, Inc.Inventors: Phil Belgrader, Christopher G. Cooney, Robert Doebler, Anna Hickerson, Bruce Irvine, Ali Nadim, James Sterling, Reza Miraghaie