Abstract: The present disclosure relates to an improved method based on hormone-receptor binding for the determination of follicle stimulating hormone and to improved reagents useful for the determination of follicle stimulating hormone in a hormone-receptor binding assay.
Type:
Grant
Filed:
June 25, 1986
Date of Patent:
May 1, 1990
Assignee:
The Albany Medical College of Union University
Inventors:
Alan L. Schneyer, Patrick M. Sluss, Bosukonda Dattatreyamurty, Leo E. Reichert, Jr.
Abstract: The present disclosure relates to novel liposome compositions and methods for their preparation. Utilization of the present invention provides an efficient reconstitution of membrane proteins into large (0.1 to 2 micron diameter) phospholipid vesicles with a large, internal aqueous space. The method has been exemplified with the use of glycoproteins of influenza (A/PR8/34) and Sendai (parainfluenza type I) viruses. The method comprises (A) extracting out the desired membrane protein from a source biological material with an extraction buffer comprising a detergent; (B) mixing the extract with a phospholipid solution and deriving a cochleate intermediate; and (C) forming large phospholipid vesicles with integrated membrane protein in a biologically active state.
Type:
Grant
Filed:
April 13, 1987
Date of Patent:
October 3, 1989
Assignee:
Albany Medical College of Union University