Patents Assigned to Alere San Diego, Inc.
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Patent number: 11628216Abstract: The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.Type: GrantFiled: November 29, 2021Date of Patent: April 18, 2023Assignee: ALERE SAN DIEGO, INC.Inventors: Mariusz Banaszczyk, Normand Hébert, Neil Stowe
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Patent number: 11192857Abstract: The present invention relates to novel 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.Type: GrantFiled: May 18, 2017Date of Patent: December 7, 2021Assignee: ALERE SAN DIEGO, INC.Inventors: Fei Huang, Dongpei Wu, Lupe Mejorado, Mariusz Banaszczyk
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Patent number: 11185864Abstract: A manually actuated chromatography device comprising a chamber for receiving a liquid sample, a pump with a metering valve, and a chromatography element, wherein the pump moves a predetermined volume of liquid from the sample chamber to the chromatography element.Type: GrantFiled: October 31, 2016Date of Patent: November 30, 2021Assignees: ALERE SAN DIEGO, INC., ALERE SWITZERLAND GMBHInventors: Daniel Nicholas Haworth, John Paul Palmer-Felgate
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Patent number: 10632464Abstract: A microfluidic device includes an inlet port configured to receive a sample, a first reaction chamber fluidically coupled to the inlet port, a first pump fluidically coupled to the inlet port, a second pump fluidically coupled to a mixing chamber, a metering channel fluidically coupled to the first reaction chamber and to the mixing chamber, and one or more second reaction chambers fluidically coupled to the mixing chamber. The first pump is configured to move fluid from the inlet port to the first reaction chamber and from the first pump to the inlet port. The second pump is configured to move fluid from the second pump to the mixing chamber, from the first reaction chamber to the mixing chamber, and from the mixing chamber to the one or more second reaction chambers.Type: GrantFiled: February 27, 2018Date of Patent: April 28, 2020Assignee: ALERE SAN DIEGO, INC.Inventors: Alexander Schenk zu Schweinsberg, Austin Matthew Derfus, Justin Davidson, Karthikeyan Kumaravadivelu, Maulik Vinod Patel, Olaf Piepenburg, Catherine Jean Greenwood, Oliver Nentwich
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Patent number: 10538760Abstract: This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.Type: GrantFiled: December 11, 2015Date of Patent: January 21, 2020Assignee: ALERE SAN DIEGO, INC.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes
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Publication number: 20190292148Abstract: The present invention relates to novel 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.Type: ApplicationFiled: May 18, 2017Publication date: September 26, 2019Applicant: ALERE SAN DIEGO, INC.Inventors: Fei HUANG, Dongpei WU, Lupe MEJORADO, Mariusz BANASZCZYK
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Patent number: 10329603Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes.Type: GrantFiled: April 25, 2017Date of Patent: June 25, 2019Assignee: ALERE SAN DIEGO INC.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Patent number: 10329602Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundres of megabases in length.Type: GrantFiled: December 30, 2016Date of Patent: June 25, 2019Assignee: Alere San Diego, Inc.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Publication number: 20190056386Abstract: The present invention describes compositions and methods for treating cardiovascular disease and myocardial infarction using dipeptidyl peptidase inhibitors. Also provided are methods for increasing natriuretic peptide function by administering one or more analogues of B type natriuretic peptide that provide increased stability in the presence of prolyl-specific dipeptidyl peptidases.Type: ApplicationFiled: May 21, 2018Publication date: February 21, 2019Applicant: ALERE SAN DIEGO, INC.Inventors: Michael A. Whittaker, Kenneth F. Buechler
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Publication number: 20180353597Abstract: The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.Type: ApplicationFiled: August 20, 2018Publication date: December 13, 2018Applicant: ALERE SAN DIEGO, INC.Inventors: Mariusz BANASZCZYK, Normand HÉBERT, Neil STOWE
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Patent number: 10093908Abstract: The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.Type: GrantFiled: December 19, 2013Date of Patent: October 9, 2018Assignee: Alere San Diego, Inc.Inventors: Olaf Piepenburg, Niall A. Armes, Mathew James David Parker
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Patent number: 10052379Abstract: The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.Type: GrantFiled: July 3, 2017Date of Patent: August 21, 2018Assignee: ALERE SAN DIEGO, INC.Inventors: Mariusz Banaszczyk, Normand Hébert, Neil Stowe
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Patent number: 10036057Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes.Type: GrantFiled: September 25, 2015Date of Patent: July 31, 2018Assignee: ALERE SAN DIEGO, INC.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Patent number: 9977013Abstract: Methods for improving immunoassays, and in particular, assays for B-Type natriuretic peptide analogues resistant to prolyl-specific dipeptidyl degradation are provided wherein one or more antibodies are selected such that the one or more antibodies detect a biologically active form of a natriuretic peptide of interest.Type: GrantFiled: October 13, 2016Date of Patent: May 22, 2018Assignee: ALERE SAN DIEGO, INC.Inventors: Michael A. Whittaker, Kenneth F. Buechler
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Patent number: 9932577Abstract: This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.Type: GrantFiled: October 29, 2013Date of Patent: April 3, 2018Assignee: ALERE SAN DIEGO, INC.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes
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Patent number: 9896719Abstract: A new class of nucleic acid substrates for AP endonucleases and members of the glycosylase/lyase family of enzymes is described. Representatives of each family, the enzymes Nfo and fpg, respectively, cleave nucleic acid backbones at positions in which a base has been replaced by a linker to which a variety of label moieties may be attached. The use of these synthetic substrates embedded within oligonucleotides is of utility in a number of applications.Type: GrantFiled: October 3, 2016Date of Patent: February 20, 2018Assignee: Alere San Diego Inc.Inventors: Olaf Piepenburg, Niall A. Armes
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Patent number: 9719132Abstract: A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.Type: GrantFiled: August 31, 2015Date of Patent: August 1, 2017Assignee: Alere San Diego Inc.Inventors: Niall A. Armes, Olaf Piepenburg, Catherine Jean Greenwood
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Patent number: 9694069Abstract: The present invention relates to novel 6-acetylmorphine analogs, and methods for their synthesis and use. Such analogs are designed to provide a convenient linkage chemistry for coupling under mild conditions to a suitable group on a target protein, polypeptide, solid phase or detectable label.Type: GrantFiled: March 14, 2014Date of Patent: July 4, 2017Assignee: ALERE SAN DIEGO, INC.Inventors: Mariusz Banaszczyk, Normand Hébert, Neil Stowe
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Patent number: 9663820Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes.Type: GrantFiled: January 6, 2016Date of Patent: May 30, 2017Assignee: Alere San Diego Inc.Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Publication number: 20170138932Abstract: The present invention describes compositions and methods for treating cardiovascular disease and myocardial infarction using dipeptidyl peptidase inhibitors. Also provided are methods for increasing natriuretic peptide function by administering one or more analogues of B type natriuretic peptide that provide increased stability in the presence of prolyl-specific dipeptidyl peptidases.Type: ApplicationFiled: October 13, 2016Publication date: May 18, 2017Applicant: ALERE SAN DIEGO, INC.Inventor: Michael A. Whittaker