Abstract: Methods for increasing the genetic stability of genetically enhanced microbial host cells capable of producing a compound of interest are disclosed.
Type:
Grant
Filed:
June 14, 2017
Date of Patent:
January 8, 2019
Assignee:
Algenol Biotech LLC
Inventors:
Ming-De Deng, Ulf Dühring, Frank Uliczka, Kimberly Lynn Anderson
Abstract: The invention provides genetically modified cyanobacterial cells that are capable of utilizing phosphite as a primary phosphorus source, and can out-compete contaminant organisms for certain forms of phosphorus more effectively.
Type:
Grant
Filed:
October 12, 2017
Date of Patent:
November 27, 2018
Assignee:
Algenol Biotech LLC
Inventors:
Paul Gordon Roessler, Charles Ryan Budinoff, Songhua Zhu, Kui Wang, Heike Enke, Christian Weissert, Frank Jochem, Ming-De Deng
Abstract: Methods for extracting phycocyanin from biomass, comprising suspending dried biomass in a buffer solution, separating the biomass from supernatant, including through centrifugation and/or filtration, and purifying the supernatant, including through filtration.
Type:
Application
Filed:
April 24, 2018
Publication date:
October 25, 2018
Applicant:
Algenol Biotech LLC
Inventors:
Charles Budinoff, Lauren Hehman, Kevin Sweeney, Michael D. McConnell, Monica R. Stegman
Abstract: The invention provides genetically modified cyanobacterial cells that are capable of utilizing phosphite as a primary phosphorus source, and can out-compete contaminant organisms for certain forms of phosphorus more effectively.
Type:
Application
Filed:
October 12, 2017
Publication date:
April 26, 2018
Applicant:
Algenol Biotech LLC
Inventors:
Paul Gordon Roessler, Charles Ryan Budinoff, Songhua Zhu, Kui Wang, Heike Enke, Christian Weissert, Frank Jochem, Ming-De Deng
Abstract: A genetically enhanced cyanobacterial host cell, Cyanobacterium sp. ABICyanoI, is disclosed. The enhanced Cyanobacterium sp. ABICyanoI produces a compound or compounds of interest.
Type:
Grant
Filed:
December 23, 2013
Date of Patent:
January 9, 2018
Assignee:
Algenol Biotech LLC
Inventors:
Kui Wang, Tuo Shi, Irina Piven, Masami Inaba, Frank Uliczka, Dan Kramer, Heike Enke, Kerstin Baier, Alexandra Friedrich, Ulf Duehring
Abstract: Methods for increasing the genetic stability of genetically enhanced microbial host cells capable of producing a compound of interest are disclosed.
Type:
Application
Filed:
June 14, 2017
Publication date:
October 5, 2017
Applicant:
Algenol Biotech LLC
Inventors:
Ming-De DENG, Ulf DÜHRING, Frank ULICZKA, Kimberly Lynn ANDERSON
Abstract: A non-naturally occurring cyanobacterial cell for the production of a chemical compound of interest is provided which contains at least one genetic modification that alters expression of at least one phosphate uptake regulating gene encoding a protein involved in regulation of phosphate metabolism, so that cellular uptake and/or intracellular storage of a phosphate compound is increased in comparison to a native form of the cyanobacterial cell. Related methods and uses involving the cyanobacterial cell are also provided.
Type:
Application
Filed:
December 22, 2016
Publication date:
June 22, 2017
Applicant:
Algenol Biotech LLC
Inventors:
Dan Kramer, Christian Weissert, John Coleman, Heike Enke, Eva Schunder, Charles Ryan Budinoff, William Patrick Porubsky, Laura Belicka
Abstract: The invention provides novel compositions of matter for the production of ethanol from carbon dioxide and water. Particularly, the invention provides photoautotrophic organisms having a first and second genetic modification, wherein the first genetic modification improves the ethanol production from organisms having the second genetic modification.
Type:
Grant
Filed:
February 9, 2009
Date of Patent:
May 16, 2017
Assignee:
Algenol Biotech LLC
Inventors:
R. Paul Woods, Craig R. Smith, Dan Kramer, Heike Enke, Ulf Duhring, Kerstin Baier, Marianne Grundel, Wolfgang Lockau, Karl Ziegler, Christine Oesterhelt, John Coleman
Abstract: One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.
Type:
Grant
Filed:
June 30, 2014
Date of Patent:
January 24, 2017
Assignee:
Algenol Biotech LLC
Inventors:
Ulf Dühring, Kerstin Baier, Frauke Germer, Tuo Shi
Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.
Type:
Grant
Filed:
May 21, 2015
Date of Patent:
November 15, 2016
Assignee:
Algenol Biotech LLC
Inventors:
Ulf Dühring, Heike Enke, Karl Ziegler, Torsten Schwecke
Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.
Type:
Grant
Filed:
May 21, 2015
Date of Patent:
November 15, 2016
Assignee:
Algenol Biotech LLC
Inventors:
Ulf Dühring, Heike Enke, Karl Ziegler, Torsten Schwecke
Abstract: A plasmid vector for the production of compounds in cyanobacteria is described which is capable of being efficiently transformed to and replicating in a broad range of cyanobacterial species.
Abstract: One embodiment of the invention provides a genetically enhanced Chlorogloeopsis sp. host cell comprising at least one first recombinant gene encoding a first protein for the production of ethanol under the transcriptional control of a first inducible promoter, having at least 85%, 90% or 95% sequence identity to an endogenous inducible promoter of the Chlorogloeopsis sp. host cell.
Type:
Grant
Filed:
August 28, 2015
Date of Patent:
July 26, 2016
Assignee:
Algenol Biotech LLC
Inventors:
Alexandra Friedrich, Irina Piven, Frank Uliczka, Heike Enke
Abstract: Silicalite particles, methods of making silicalite particles, systems comprising silicalite particles and methods for using silicalite particles to separate ethanol from water.
Type:
Grant
Filed:
August 5, 2015
Date of Patent:
May 3, 2016
Assignee:
Algenol Biotech LLC
Inventors:
Ryan Lively, Michelle Dose, Ronald Chance, Benjamin McCool
Abstract: A cyanobacterial host cell, Cyanobacterium sp., that harbors at least one recombinant gene for the production of a chemical compounds is provided, as well as vectors derived from an endogenous plasmid isolated from the cell.
Type:
Grant
Filed:
June 5, 2014
Date of Patent:
April 19, 2016
Assignee:
Algenol Biotech LLC
Inventors:
Irina Piven, Alexandra Friedrich, Ulf Dühring, Frank Uliczka, Kerstin Baier, Masami Inaba, Tuo Shi, Kui Wang, Heike Enke, Dan Kramer
Abstract: This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds.
Type:
Grant
Filed:
June 30, 2014
Date of Patent:
April 19, 2016
Assignee:
Algenol Biotech LLC
Inventors:
Ulf Dühring, Alexandra Friedrich, Kerstin Baier, Heike Enke, Dan Kramer