Abstract: The present invention relates to a DNA sequence, which codes for endomannanase produced by fungi of the genus Trichoderma and transferred into a yeast or fungus strain induces that strain to produce endomannanase, as well as to a method for isolating genes coding for endomannanases. The invention also relates to vectors, yeast strains and fungal strains containing the DNA sequence. Furthermore, the invention provides an enzyme product containing at least one endomannanase, which contains at least one of the following endomannanases produced by fungi of the Trichoderma genus: an enzyme having mannanase activity and an isoelectric point (pI) of about 3.8, an enzyme having mannanase activity and a pI of about 4.1, an enzyme having mannanase activity and a pI of about 4.5, an enzyme having mannanase activity and a pI of about 5.4 and an enzyme having mannanase activity and a pI of about 6.5, the isoelectric points being determined by isoelectric focusing.

Abstract: A novel high density liquid or paste yeast preparation, which comprises approximately 1-20% (w/w) of a polyol selected from propylene glycol, glycerol, nonfermentable mono- or oligosaccharides or sugar alcohols such as xylose, mannitol and sorbitol, soluble oligo- or polymeric carbohydrates such as partially hydrolysed starch, cellulose, agarose, or polyethylene glycol, or mixtures thereof, and fresh yeast. The high density yeast preparation has a density of more than 800 g yeast (of dry matter 27-29 %) per liter of preparation, and the concentration of the polyol in the extracellular liquid is 5-50 % (w/v). The preparation has an improved capacity for retaining its activity, dissolving instantly, and for being easily batched. It is uniformily suspendable and tolerant of repeated freezing and thawing. The liquid high density yeast preparation has a viscosity of less than 200 centiPoise (cP) at 20.degree. C.

Abstract: A method for treating alcoholism by extinguishing the alcohol-drinking response in which an opiate antagonist is transdermally administered to a subject and a device for transdermally administering the antagonist. The device is a package containing a fixed dose of opiate antagonist, a vehicle and a permeation enhancer.

Abstract: A method for treating alcoholism. The alcohol-drinking response of alcoholics is extinguished by having them drink alcoholic beverages while nalmefene, an opiate antagonist, blocks the positive reinforcement effect of ethanol in the brain.

Abstract: A smooth textured composition of yeast cells, more than 50% of the yeast cells comprising whole yeast cell walls, the composition comprising at least about 40% by weight of fiber and less than about 1.6% by weight of nucleic acid.

Abstract: The objects of this invention are new Saccharomyces cerevisiae yeast strains into which .alpha.-galactosidase gene (MEL.sup.+) has been transferred by using recombinant DNA methods. Baker's and distiller's yeasts producing .alpha.-galactosidase, are utilizable in the corresponding industry, because they are able to utilize the raffinose present in molasses, which results in greater yield of yeast (or ethanol) and reduction or elimination of the costs associated with biological oxygen demand (B.O.D.) in the effluent from factories. The improved ability of brewer's yeasts to produce .alpha.-galactosidase provides a sensitive method for monitoring pasteurization of beer.The new yeast strains prepared by using recombinant DNA methods produce more .alpha.-galactosidase than naturally occurring .alpha.-galactosidase producing yeast strains.Also methods for marking yeast strains and for producing stable transformants of yeasts are presented.

Abstract: A process for the production of starch from cereals includes the steps (a) subjecting dry-ground dehusked grains to enzyme treatment, (b) homogenizing the treated product (optional), (c) extracting starch from the homogenized product, leaving the fibre, (d) concentrating the starch to yield a crude starch fraction and process water, (e) separating proteins from the crude starch and (f) separating the crude starch into primary and secondary starch fractions. The process assures that solubles will be maximized so that drying can be accomplished primarily by evaporators rather than by other types of dryers.

Abstract: A liquid dispenser for being affixed to the neck of a liquid-filled container or bottle comprises a cap having a spout and a vent, an inner core for insertion in a cylindrical inner cavity of the cap and a stopper assembly attached to the inner core. The cap is rotatable about the inner core and stopper assembly between an open and closed position. Dispensing channels and venting channels in the core and stopper assembly align with the spout and vent in the cap in the open position. The dispensing channels and venting channels are disconnected from the spout and vent in the closed position. Various provisions are made so that the dispenser will be leak-proof in storage and in use. A tamper-evident arrangement is included in a portion of the dispenser. Specific features are provided in the neck of a bottle upon which the dispenser is to be affixed in order to position and lock the dispenser in place.