Abstract: A method and device for performing direct quantitative real time PCR in a crude sample (200), wherein said sample is subjected to a centrifugal force sufficient to separate components of the sample into a supernatant and a pellet, and wherein at least one light source (204) and at least one detector (205) are positioned so that the excitation light impinges on the sample in a position above said pellet, and said detector detects light emitted from a position above said pellet.
Abstract: A method and device for performing direct quantitative real time PCR in a crude sample (200), wherein said sample is subjected to a centrifugal force sufficient to separate components of the sample into a supernatant and a pellet, and wherein at least one light source (204) and at least one detector (205) are positioned so that the excitation light impinges on the sample in a position above said pellet, and said detector detects light emitted from a position above said pellet.
Abstract: A rotor assembly for centrifuging reaction vessels containing reaction mixtures in a device for asymmetric heating and cooling of reaction mixtures during centrifugation. The rotor contains at least one fan blade used to force ambient gas to pass the reaction mixture. Also provided is a device for asymmetric heating and cooling of reaction mixtures during centrifugation.
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