Abstract: A novel preparative methodology yields water-soluble, cross-linked conjugates and conjugate complexes that confer an improved sensitivity in immunochemical assays, particularly in the context of lateral flow devices and in determinations of the presence or absence of small amounts of active components present in a liquid sample.
Type:
Grant
Filed:
April 5, 2001
Date of Patent:
September 30, 2003
Assignee:
Amdex A/S
Inventors:
Allan Otto Fog Lihme, Christopher John Stanley
Abstract: A nucleic acid primer is bound to a soluble carrier macromolecule, a multitude of primer molecules thus being carried by each carrier macromolecule, and hybridisation of the primer to a template followed by extension of the primer to replicate the template in complementary form is carried out as part of a PCR procedure or other amplification, or to form an extended primer of greater hybridisation affinity. A second primer used in the amplification may also be bound to a carrier macromolecule.
Abstract: A nucleic acid primer is bound to a soluble carrier macromolecule, a multitude of primer molecules thus being carried by each carrier macromolecule, and hybridisation of the primer to a template followed by extension of the primer to replicate the template in complementary form is carried out as part of a PCR procedure or other amplification, or to form an extended primer of greater hybridization affinity. A second primer used in the amplification may also be bound to a carrier macromolecule.
Abstract: Method for detecting the presence or absence of a viable microorganism in a sample which has been subjected to a sterilization process, whereby the sample is contacted with an antibody which specifically recognizes and binds an indicator epitope found on the surface of an intact viable microorganism, but not on the surface of a microorganism killed by the sterilization process.
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