Patents Assigned to Amnis Corporation
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Patent number: 8150136Abstract: The use of an imaging system, cell compartment markers, and molecular markers in methods for correlating the movement of molecules within a cell to a particular compartment are provided, including measuring and correlating molecule movement in adherent and non-adherent cells.Type: GrantFiled: March 16, 2005Date of Patent: April 3, 2012Assignee: Amnis CorporationInventors: Thaddeus C. George, David A. Basiji, Keith Frost, Brian E. Hall, William E. Ortyn, Michael J. Seo
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Patent number: 8131053Abstract: Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images for each cell passing through the field of view of the imaging system. Acquiring a plurality of different images (i.e., bright field, dark field, and fluorescent images) facilitates the determination of different morphological and morphometric parameters. Simultaneously acquiring the plurality of images enables relatively large populations of cells to be rapidly imaged, so that relatively small numbers of cancer cells in a large population of cells can be detected. Initially, known cancer cells are imaged to enable a marker to be identified. Then, a sample that may include cancer cells is imaged to determine if the marker is present.Type: GrantFiled: July 28, 2008Date of Patent: March 6, 2012Assignee: Amnis CorporationInventors: William Ortyn, David Basiji, Luchuan Liang, Vidya Venkatachalam, Philip Morrissey
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Patent number: 8103080Abstract: Provided are methods for determining and analyzing photometric and morphogenic features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.Type: GrantFiled: March 16, 2005Date of Patent: January 24, 2012Assignee: Amnis CorporationInventors: Thaddeus C George, David A Basiji, Brian E Hall, William E Ortyn, Michael J Seo, Philip J Morrissey, Cathleen A Zimmerman
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Publication number: 20120013785Abstract: A system and method for high numeric aperture imaging systems includes a splitter, a defocusing system, and a combiner. The splitter reflects a portion of collected light and transmits another portion of the collected light. The defocusing system is configured to modify optical power of either the transmitted portion or reflected portion of the collected light. The combiner is oriented with respect to a mechanical angle. The combiner recombines portions of the transmitted portion and the reflected portion such that the transmitted portion and reflected portion are subsequently transmitted being separated by an optical separation angle based upon the mechanical angle of orientation of the combiner. Various other implementations are used to maintain focus with regards to the imaging systems involved.Type: ApplicationFiled: February 14, 2011Publication date: January 19, 2012Applicant: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, David J. Perry
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Publication number: 20110280467Abstract: Provided are methods for determining and analyzing photometric and morphogenic features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis.Type: ApplicationFiled: July 26, 2011Publication date: November 17, 2011Applicant: Amnis CorporationInventors: Thaddeus C. George, David A. Basiji, Brian E. Hall, William E. Ortyn, Michael J. Seo, Philip J. Morrissey, Cathleen A. Zimmerman
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Patent number: 8009189Abstract: A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.Type: GrantFiled: June 3, 2008Date of Patent: August 30, 2011Assignee: Amnis CorporationInventors: William Ortyn, David Basiji, Keith Frost, Luchuan Liang, Richard Bauer, Brian Hall, David Perry
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Patent number: 8005314Abstract: A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.Type: GrantFiled: December 11, 2006Date of Patent: August 23, 2011Assignee: Amnis CorporationInventors: William Ortyn, David Basiji, Keith Frost, Luchuan Liang, Richard Bauer, Brian Hall, David Perry
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MODIFYING THE OUTPUT OF A LASER TO ACHIEVE A FLAT TOP IN THE LASER'S GAUSSIAN BEAM INTENSITY PROFILE
Publication number: 20110085221Abstract: A laser beam is periodically deflected before being directed into a sample volume. The beam is deflected at a frequency such that the beam makes one or more passes through the sample volume while data are collected from the sample volume. The amplitude of motion of the beam, the dwell time of the beam at any given point, and the Gaussian intensity profile of the beam cooperate to produce an effective flat topped illumination profile for the light that is incident on specimens in the sample volume. The total photon exposure at any given point in the sample volume is a function of both the beam intensity and the dwell time at that location. Therefore, a longer dwell time and lower intensity at the edge of the profile are in balance with a shorter dwell time and higher intensity at the center of the profile.Type: ApplicationFiled: September 20, 2010Publication date: April 14, 2011Applicant: Amnis CorporationInventors: William Ortyn, David Perry, Tom Montague -
Patent number: 7925069Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.Type: GrantFiled: September 29, 2009Date of Patent: April 12, 2011Assignee: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, Philip Morrissey, Thaddeus George, Brian Hall, Cathleen Zimmerman, David Perry
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Patent number: 7889263Abstract: A system and method for high numeric aperture imaging systems includes a splitter, a defocusing system, and a combiner. The splitter reflects a portion of collected light and transmits another portion of the collected light. The defocusing system is configured to modify optical power of either the transmitted portion or reflected portion of the collected light. The combiner is oriented with respect to a mechanical angle. The combiner recombines portions of the transmitted portion and the reflected portion such that the transmitted portion and reflected portion are subsequently transmitted being separated by an optical separation angle based upon the mechanical angle of orientation of the combiner. Various other implementations are used to maintain focus with regards to the imaging systems involved.Type: GrantFiled: April 5, 2010Date of Patent: February 15, 2011Assignee: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, David J. Perry
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Publication number: 20100240062Abstract: The present invention provides methods for preparing cells with highly condensed chromosomes, such as sperm, and methods for detecting and quantifying specific cellular target molecules in intact cells. Specifically, methods are provided for detecting chromosomes and chromosomal abnormalities, including aneuploidy, in intact cells using fluorescence in situ hybridization of cells in suspension, such as sperm cells.Type: ApplicationFiled: May 28, 2010Publication date: September 23, 2010Applicant: Amnis CorporationInventors: James Brawley, Philip J. Morrissey, Rosalynde J. Finch, David A. Basiji, Luchuan Liang
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Publication number: 20100232675Abstract: Multimodal or multispectral images of cells comprising a population of cells are simultaneously collected. Photometric and/or morphometric image features identifiable in the images are used to identify differences between first and second populations of cells. The differences can include changes in a relative percentage of different cell types in each population, or a change in a first type of cell present in the first population of cells and the same type of cell in the second population of cells. The changes may be indicative of a disease state, indicative of a relative effectiveness of a therapy, or indicative of a health of the person from whom the cells populations were obtained.Type: ApplicationFiled: December 4, 2009Publication date: September 16, 2010Applicant: Amnis CorporationInventors: William Ortyn, David Basiji, Philip Morrissey, Thaddeus George, Brian Hall, Cathleen Zimmerman, David Perry
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Publication number: 20100188559Abstract: A system and method for high numeric aperture imaging systems includes a splitter, a defocusing system, and a combiner. The splitter reflects a portion of collected light and transmits another portion of the collected light. The defocusing system is configured to modify optical power of either the transmitted portion or reflected portion of the collected light. The combiner is oriented with respect to a mechanical angle. The combiner recombines portions of the transmitted portion and the reflected portion such that the transmitted portion and reflected portion are subsequently transmitted being separated by an optical separation angle based upon the mechanical angle of orientation of the combiner. Various other implementations are used to maintain focus with regards to the imaging systems involved.Type: ApplicationFiled: April 5, 2010Publication date: July 29, 2010Applicant: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, David J. Perry
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Patent number: 7719598Abstract: A system and method for high numeric aperture imaging systems includes a splitter, a defocusing system, and a combiner. The splitter reflects a portion of collected light and transmits another portion of the collected light. The defocusing system is configured to modify optical power of either the transmitted portion or reflected portion of the collected light. The combiner is oriented with respect to a mechanical angle. The combiner recombines portions of the transmitted portion and the reflected portion such that the transmitted portion and reflected portion are subsequently transmitted being separated by an optical separation angle based upon the mechanical angle of orientation of the combiner. Various other implementations are used to maintain focus with regards to the imaging systems involved.Type: GrantFiled: January 24, 2006Date of Patent: May 18, 2010Assignee: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, David J. Perry
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Publication number: 20100021039Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.Type: ApplicationFiled: September 29, 2009Publication date: January 28, 2010Applicant: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, Philip Morrissey, Thaddeus George, Brian Hall, Cathleen Zimmerman, David Perry
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Patent number: 7634126Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.Type: GrantFiled: January 29, 2009Date of Patent: December 15, 2009Assignee: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, Philip Morrissey, Thaddeus George, Brian Hall, Cathleen Zimmerman, David Perry
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Patent number: 7634125Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.Type: GrantFiled: August 20, 2008Date of Patent: December 15, 2009Assignee: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, Philip Morrissey, Thaddeus George, Brian Hall, Cathleen Zimmerman, David Perry
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Patent number: 7610942Abstract: A low pulsatility syringe pump including a duplex bearing set rotatingly supporting a lead screw, and a transmission having a first drive train configured to increase a number of motor rotations required for a single rotation of the lead screw, and a second drive train configured to reduce the number of motor rotations required for a single rotation of the lead screw as compared to the first drive train. Another embodiment also includes a motor configured to rotate the syringe about its own axis, independent of the motion of the lead screw. In this other embodiment, the fluid in the syringe barrel includes objects (such as cells, latex beads, etc.) entrained in the fluid. The rate of rotation (e.g., about three revolutions per second) is chosen such that each object traces a substantially circular pathway in the syringe barrel and remains in suspension.Type: GrantFiled: January 15, 2004Date of Patent: November 3, 2009Assignee: Amnis CorporationInventors: Norio Harui, Michael A. Crawford, Richard J. Esposito, William E. Ortyn
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Publication number: 20090202130Abstract: The use of an imaging system, cell compartment markers, and molecular markers in methods for correlating the movement of molecules within a cell to a particular compartment are provided, including measuring and correlating molecule movement in adherent and non-adherent cells.Type: ApplicationFiled: March 16, 2005Publication date: August 13, 2009Applicant: AMNIS CORPORATIONInventors: Thaddeus C. George, David A. Basiji, Keith Frost, Brian E. Hall, William E. Ortyn, Michael J. Seo
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Publication number: 20090190822Abstract: Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.Type: ApplicationFiled: January 29, 2009Publication date: July 30, 2009Applicant: Amnis CorporationInventors: William E. Ortyn, David A. Basiji, Philip Morrissey, Thaddeus George, Brian Hall, Cathleen Zimmerman, David Perry