Patents Assigned to AnaSpec, Inc.
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Publication number: 20170226159Abstract: A method for producing a purified peptide from a supported crude peptide having a support and a first peptide chain bonded to the support at the C-terminus. The method includes: introducing a linker and a hydrophilic unit to an amino group of the first peptide chain of the supported crude peptide; cleaving a bond between the first peptide chain and the support before or after at least one of the linker and the hydrophilic unit is introduced to the amino group of the first peptide chain such that a support-free hydrophilized peptide is obtained; treating the support-free hydrophilized peptide by liquid chromatography; and cleaving a bond between the linker and the first peptide chain in the support-free hydrophilized peptide by chemical treatment after the liquid chromatography treatment such that a peptide including the first peptide chain is obtained.Type: ApplicationFiled: September 25, 2015Publication date: August 10, 2017Applicants: KANEKA CORPORATION, AnaSpec, Inc.Inventors: Keishi TAKATSU, Hirofumi MAEDA, Akio FUJII, Fengying LI, Xoapbing WANG
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Patent number: 7754893Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and the 3-position, their bioconjugates and their uses are described. 1,3?-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1?-crosslinked or non-crosslinked dyes. The invention includes derivate compounds having one or more benzo nitrogens.Type: GrantFiled: October 6, 2008Date of Patent: July 13, 2010Assignee: AnaSpec, Inc.Inventors: Zhenjun Diwu, Jianheng Zhang, Yi Tang, Xiang Guobing
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Publication number: 20100029954Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3?-position, their bioconjugates and their uses are described. 1,3?-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1?-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.Type: ApplicationFiled: August 20, 2009Publication date: February 4, 2010Applicant: AnaSpec Inc.Inventors: Zhenjun Diwu, Jianheng Zhang, Yi Tang, Xiang Guobing
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Publication number: 20100022768Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3?-position, their bioconjugates and their uses are described. 1,3?-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1?-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.Type: ApplicationFiled: August 20, 2009Publication date: January 28, 2010Applicant: AnaSpec IncInventors: Zhenjun Diwu, Jianheng Zhang, Yi Tang, Xiang Guobing
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Patent number: 7465810Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3?-position, their bioconjugates and their uses are described. 1,3?-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1?-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.Type: GrantFiled: October 21, 2005Date of Patent: December 16, 2008Assignee: AnaSpec, Inc.Inventors: Zhenjun Diwu, Jianheng Zhang, Yi Tang, Xiang Guobing
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Publication number: 20060223076Abstract: The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling a wide variety of substances, including biomolecules. These labeled substances are highly useful for a variety of energy-transfer assays and applications.Type: ApplicationFiled: September 7, 2005Publication date: October 5, 2006Applicant: AnaSpec, Inc.Inventors: Zhenjun Diwu, Jianheng Zhang, Yi Tang
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Publication number: 20060088816Abstract: The present invention relates generally to the detection and measurement of transmembrane potentials using an N,N,N?-trialkyl thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a slightly hydrophobic N,N,N?-trialkyl thiobarbituric acid-derived polymethine oxonols. The method comprises a slightly hydrophobic N,N,N?-trialkyl thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.Type: ApplicationFiled: August 25, 2005Publication date: April 27, 2006Applicant: AnaSpec, Inc.Inventors: Zhenjun Diwu, Jianjun He, Yi Tang
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Publication number: 20050226816Abstract: The present invention relates generally to the detection and measurement of transmembrane potentials using an asymmetric thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonols. The method comprises a moderately hydrophobic asymmetric thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes.Type: ApplicationFiled: October 21, 2004Publication date: October 13, 2005Applicant: AnaSpec, Inc.Inventors: Zhenjun Diwu, Yong Yao, Jianjun He, Guobing Xiang