Abstract: This Invention relates to, for production of N-acetyl-D-glucosamine and D-glucosamine by microbial fermentation: A nongenetic recombinant strain, preserved in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms; NJ090259, a strain of Bacillus subtilis, Preservation No. CGMCC10257; and NJ091195, a strain of Bacillus licheniformis, Preservation No. CGMCC10258 and Preservation Date Dec. 29, 2014. The beneficial effects of this Invention are: Provide a new strain for production of N-acetyl-D-glucosamine and D-glucosamine, and its production methods. By this method, it may achieve stable production and supply of N-acetyl-D-glucosamine, and may also achieve non-animal-derived, safety production of N-acetyl-D-glucosamine and D-glucosamine; the method is of short production phase and low cost, and is more environmentally-friendly.