Abstract: The present invention relates to methods of preparing biological material, for use in various experimental, diagnostic or therapeutic uses, including immunotherapy treatment or prophylaxy of tumors. More particularly, the present invention relates to methods of preparing membrane vesicles (in particular exosomes) released by various types of mammalian cells, comprising diafiltration and/or density cushion centrifugation. The invention also provides novel methods for characterizing and analyzing exosome preparations, which can be used in quality control assay for the purpose of pharmaceutical product production. The invention is suitable to produce pharmaceutical grade preparations of such membrane vesicles and to fully characterize said preparations, for use in human beings.
Type:
Grant
Filed:
April 27, 2000
Date of Patent:
November 2, 2004
Assignee:
Anosys, Inc.
Inventors:
Henry Lamparski, Curtis Ruegg, Jean-Bernard Le Pecq, Di-Hewi Hsu, Jenq Yuan Yao