Abstract: Provided is a method of producing 2?-fucosyllactose including culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express ?-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
Type:
Application
Filed:
October 1, 2019
Publication date:
February 13, 2020
Applicants:
Seoul National University R&DB Foundation, AP Technologies Corp.
Inventors:
Jin-Ho SEO, Young-Wook CHIN, Hae-Yong JO
Abstract: Disclosed are a recombinant Corynebacterium glutamicum (C. glutamicum) for producing fucosyllactose which is transformed to express ?-1,2-fucosyltransferase, GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY), wherein the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and a method for producing fucosyllactose using the same. According to the recombinant Corynebacterium glutamicum and the method for producing fucosyllactose according to the present invention, with use of a GRAS Corynebacterium glutamicum strain, which is safer than conventional Escherichia coli, 2?-fucosyllactose can be produced at a high concentration while overcoming drawbacks of conventional methods associated with industrial inapplicability resulting from low production concentrations.
Type:
Application
Filed:
April 24, 2017
Publication date:
October 18, 2018
Applicants:
Seoul National University R&DB Foundation, AP Technologies Corp.
Inventors:
Jin-Ho SEO, Young-Wook CHIN, Hae-Yong JO
Abstract: An electric privacy glass having a first lite of glass with a low-emissivity coating formed using a pyrolytic process on at least one major surface and a second lite of glass with a low-emissivity coating formed using a pyrolytic process on at least one major surface is provided. The first and second lites of glass are each coated with a resin on the major surfaces having the low-emissivity coating. The first and second lites of glass are stacked such that the low-emissivity coatings are facing each other with the resin therebetween. The electric privacy glass has pressure sequentially applied. The resin in cured to adhere the first and second lites of glass. The resin can include a liquid crystal polymer and a UV-curable acrylate polymer. The UV-curable acrylate polymer can include an acrylate urethane oligomer, a high boiling point methacrylate, an acrylic acid, and a photocuring agent.
Abstract: Systems and methods are provided for causing a computing device to process a check payment from a user using a debit card by: receiving debit card information from the user; sending the information to a payment platform via a network connection; exchanging the debit card information with an ATM network at a bank and receiving a response including a bank account and a bank routing number; making a fraud potential determination; returning a success flag if fraud is not suspected and an error message if fraud is suspected; storing the returned bank account and bank routing number in a database if fraud is not suspected; requesting the user to write their signature on a digitization pad in operation and storing the signature in the database; and creating a check image.