Abstract: The invention relates to a separation free bioanalytical assay method for qualitatively and/or quantitatively determining an analyte (4) in a sample of a biological fluid or suspension. The invention resides in that the method comprises, apart from essential steps for a two-photon excitation based assay method well known in prior art, the further steps of: a) recording focus positions and corresponding two-photon excited fluorescence emission photon counts of a plurality of microparticles (1) of a device; b) calculating a correction matrix for the device employing the recorded focus positions and corresponding two-photon excited fluorescence emission photon counts, and c) correcting two-photon excited fluorescence emission photon counts from the microparticles (1) of said device employing the correction matrix obtained for the device employing the recorded focus positions and the corresponding two-photon excited fluorescence emission counts.

Abstract: The invention relates to a piercable hermetic cover (2) for a bioassay cartridge (4) with at least one reaction chamber (6). Characteristic for the invention is that: the cover (2) comprises at least a top layer (8), a middle layer (10), a bottom layer (12), and sites intended for piercing (14); and the cover (2) has, at the sites (16) intended for piercing, a hollow space (18) between the top layer (14) and the bottom layer (12). The present invention also relates to a system (20) comprising a bioassay cartridge (4) and a cover (2) for the cartridge (4). The present invention further relates to use of the cover (2) for covering the cartridge (4).

Abstract: The invention relates to a piercable hermetic cover (2) for a bioassay cartridge (4) with at least one reaction chamber (6). Characteristic for the invention is that: the cover (2) comprises at least a top layer (8), a middle layer (10), a bottom layer (12), and sites intended for piercing (14); and the cover (2) has, at the sites (16) intended for piercing, a hollow space (18) between the top layer (14) and the bottom layer (12). The present invention also relates to a system (20) comprising a bioassay cartridge (4) and a cover (2) for the cartridge (4). The present invention further relates to use of the cover (2) for covering the cartridge (4).