Patents Assigned to Associates of Cape Cod, Inc.
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Patent number: 6900059Abstract: An analytical device that can be used to conveniently and accurately assay plural vessels. In one exemplary embodiment, a pair of LED sources provides illumination through a pair of radial waveguides to plural vessels arranged in a pair of substantially concentric and circular rows about the LED sources. A light pipe receives light transmitted through a vessel from each radial waveguide and reflects the received light downward to a single printed circuit board that contains a photodiode for each light pipe, as well as processing circuitry. The first LED source/radial waveguide optical is used to confirm the presence of a vessel, and the second is used to perform, e.g., turbidometric and/or calorimetric assays upon an analyte within the vessel. The vessel is incubated in a vessel support that includes a heat conducting base and a heat insulating cover. Heat is supplied by a DC heater.Type: GrantFiled: November 27, 2000Date of Patent: May 31, 2005Assignee: Associates of Cape Cod, Inc.Inventors: Alan Shinn, Chiko Fan, Elias R. Elias, Thomas J. Novitsky, Michael Dawson, Keith Richardson
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Publication number: 20050106746Abstract: An analytical device that can be used to conveniently and accurately assay plural vessels. In one exemplary embodiment, a pair of LED sources provides illumination through a pair of radial waveguides to plural vessels arranged in a pair of substantially concentric and circular rows about the LED sources. A light pipe receives light transmitted through a vessel from each radial waveguide and reflects the received light downward to a single printed circuit board that contains a photodiode for each light pipe, as well as processing circuitry. The first LED source/radial waveguide optical is used to confirm the presence of a vessel, and the second is used to perform, e.g., turbidometric and/or colorimetric assays upon an analyte within the vessel. The vessel is incubated in a vessel support that includes a heat conducting base and a heat insulating cover. Heat is supplied by a DC heater.Type: ApplicationFiled: December 10, 2004Publication date: May 19, 2005Applicant: Associates of Cape Cod, Inc.Inventors: Alan Shinn, Chiko Fan, Elias Elias, Thomas Novitsky, Michael Dawson, Keith Richardson
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Publication number: 20050069972Abstract: Kits and method for detecting bacterial endotoxin in an aqueous solution are provided. In certain examples, the kit includes at least a first container comprising solid, endotoxin-specific, horseshoe crab amebocyte lysate and at least one buffer, whereby the sensitivity of the amebocyte lysate is pre-certified. In certain examples, the kit also contains at least a second container comprising a defined quantity of endotoxin configured as a positive product control, wherein the defined quantity of the endotoxin is pre-certified to react positively with the amebocyte lysate in the first container.Type: ApplicationFiled: July 23, 2004Publication date: March 31, 2005Applicant: Associates of Cape Cod, Inc.Inventors: Carlos Castro, Richard Ridge, Thomas Novitsky
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Publication number: 20050026239Abstract: The present invention relates to a simple, rapid, and cost-effective test kit for specifically detecting bacterial endotoxin in aqueous solutions, such as water or dialysate solutions, using a Limulus Amebocyte Lysate (LAL)-based gel clot assay. Advantageously, the test kit can vary in its level of sensitivity for detecting endotoxin.Type: ApplicationFiled: April 19, 2004Publication date: February 3, 2005Applicant: Associates of Cape Cod, Inc.Inventors: Carlos Castro, Richard Ridge, Thomas Novitsky
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Publication number: 20030087823Abstract: The present invention relates to the use of endotoxin neutralizing protein (ENP) and derivatives thereof as stand alone microbial inhibitors or as synergistic enhancers of antibiotics and preservatives. Compositions comprising ENP or alone or in combination with an antibiotic may be used to prevent or treat gram-negative bacterial infections, endotoxemia, septic shock, gram-positive bacterial infections, yeast infections and fungal infections.Type: ApplicationFiled: September 11, 2002Publication date: May 8, 2003Applicant: Associates of Cape Cod, Inc.Inventors: Erik J. Paus, Norman R. Wainwright
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Patent number: 6391295Abstract: The present invention relates to an artificial bait for marine animals, comprising cell-free hemolymph of a horseshoe crab. The cell-free hemolymph of the horseshoe crab, which is the byproduct from the manufacture of Limulus Amebocyte Lysate (LAL), is an excellent attractant to be used in artificial baits for marine animals. The baits according to the invention attract the target marine animals better than the horseshoe crab itself. Further, the baits of the invention seem to attract non-target game less than the target game. The present invention also relates to a method of preparing a bait comprising cell-free hemolymph of a horseshoe crab, and a method of attracting marine animals.Type: GrantFiled: October 25, 2000Date of Patent: May 21, 2002Assignee: Associates of Cape Cod, Inc.Inventors: Thomas J. Novitsky, Michael E. Dawson, Erik J. Paus
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Patent number: 6384200Abstract: Endotoxin binding/neutralizing proteins capable of binding endotoxin in vivo, thereby neutralizing the toxic effect or bioactivity of endotoxin which are isolated from a horseshoe crab such as Limulus polyphemus, pharmaceutical compositions and pharmaceutical uses of the proteins, a method of purifying the proteins and an assay for endotoxin based on the proteins, are disclosed.Type: GrantFiled: May 1, 1997Date of Patent: May 7, 2002Assignee: Associates of Cape Cod, Inc.Inventors: Norman R. Wainwright, Thomas J. Novitsky
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Patent number: 6222021Abstract: Endotoxin binding/neutralizing proteins capable of binding endotoxin in vivo, thereby neutralizing the toxic effect or bioactivity of endotoxin which are isolated from a horseshoe crab such as Limulus polyphemus, pharmaceutical compositions and pharmaceutical uses of the proteins, a method of purifying the proteins and an assay for endotoxin based on the proteins, are disclosed.Type: GrantFiled: June 9, 1997Date of Patent: April 24, 2001Assignee: Associates of Cape Cod, Inc.Inventors: Norman R. Wainwright, Thomas J. Novitsky
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Patent number: 6171807Abstract: Fluorescently labeled ENP as well as methods of preparation are disclosed. Also disclosed is a method of detecting endotoxin in a liquid sample suspected of containing endotoxin, comprising admixing said liquid sample with fluorescently labeled ENP for a time sufficient to form a complex between the labeled ENP and the endotoxin, and performing fluorescence polarization experiment on the sample, wherein a change in the fluorescence polarization indicates the presence of endotoxin.Type: GrantFiled: November 10, 1997Date of Patent: January 9, 2001Assignee: Associates of Cape Cod, Inc.Inventors: Thomas J. Novitsky, Richard J. Ridge, Jack L. Sloyer
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Patent number: 5747455Abstract: Endotoxin binding/neutralizing proteins capable of binding endotoxin in vivo, thereby neutralizing the toxic effect or bioactivity of endotoxin which are isolated from a horseshoe crab such as Limulus polyphemus, pharmaceutical compositions and pharmaceutical uses of the proteins, a method of purifying the proteins and an assay for endotoxin based on the proteins, are disclosed.Type: GrantFiled: August 30, 1996Date of Patent: May 5, 1998Assignee: Associates of Cape Cod, Inc.Inventors: Norman R. Wainwright, Thomas J. Novitsky
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Patent number: 5627266Abstract: Endotoxin binding/neutralizing proteins capable of binding endotoxin in vivo, thereby neutralizing the toxic effect or bioactivity of endotoxin which are isolated from a horseshoe crab such as Limulus polyphemus, pharmaceutical compositions and pharmaceutical uses of the proteins, a method of purifying the proteins and an assay for endotoxin based on the proteins, are disclosed.Type: GrantFiled: June 7, 1995Date of Patent: May 6, 1997Assignee: Associates of Cape Cod, Inc.Inventors: Norman R. Wainwright, Thomas J. Novitsky
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Patent number: 5614369Abstract: Endotoxin binding/neutralizing proteins capable of binding endotoxin in vivo, thereby neutralizing the toxic effect or bioactivity of endotoxin which are isolated from a horseshoe crab such as Limulus polyphemus, pharmaceutical compositions and pharmaceutical uses of the proteins, a method of purifying the proteins and an assay for endotoxin based on the proteins, are disclosed.Type: GrantFiled: June 7, 1995Date of Patent: March 25, 1997Assignee: Associates of Cape Cod, Inc.Inventors: Norman R. Wainwright, Thomas J. Novitsky
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Patent number: 5594113Abstract: Endotoxin binding/neutralizing proteins capable of binding endotoxin in vivo, thereby neutralizing the toxic effect or bioactivity of endotoxin which are isolated from a horseshoe crab such as Limulus polyphemus, pharmaceutical compositions and pharmaceutical uses of the proteins, a method of purifying the proteins and an assay for endotoxin based on the proteins, are disclosed.Type: GrantFiled: June 22, 1994Date of Patent: January 14, 1997Assignee: Associates of Cape Cod, Inc.Inventors: Norman R. Wainwright, Thomas J. Novitsky
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Patent number: 4936682Abstract: An analytic instrument for kinetically measuring light absorption characteristics of a plurality of independent samples contained in disposable test tubes arranged in a circular pattern about a single light source. The instrument is designed to be used with a host personal computer and is not specific to any particular type of assay. The instrument obtains and temporarily stores raw data in the form of digitized output signals from the plurality of photodetectors and periodically passes them to the host computer. An incubator has test tube wells arranged in a circular array equidistant from a single incandescent light source. The circular symmetry controls the thermal gradients in the incubator such that all of the samples are disposed on the same isotherm and all of the photodetectors are disposed on the same isotherm.Type: GrantFiled: August 11, 1987Date of Patent: June 26, 1990Assignee: Associates of Cape Cod, Inc.Inventor: Joshua K. Hoyt
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Patent number: 4107077Abstract: Limulus lysate of improved sensitivity to endotoxin is prepared by withdrawing hemolymph from Limulus polyphemus (horseshoe crab), preparing a lysate by lysing the amoebocytes obtained from the hemolymph, treating the lysate with an amount of an organic solvent sufficient to precipitate a clotting inhibitor within the lysate and isolating the organic solvent treated lysate. In another embodiment, the sensitivity of the lysate is further increased by adding a salt of calcium, magnesium, manganese, thereto.Type: GrantFiled: August 12, 1977Date of Patent: August 15, 1978Assignee: Associates of Cape Cod, Inc.Inventors: James D. Sullivan, Jr., Stanley W. Watson
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Patent number: D443216Type: GrantFiled: July 27, 2000Date of Patent: June 5, 2001Assignee: Associates of Cape Cod, Inc.Inventors: Thomas J. Novitsky, John L. Sloyer, Jr., Elias R. Elias, Alan Shinn, Chiko Fan
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Patent number: D449894Type: GrantFiled: July 27, 2000Date of Patent: October 30, 2001Assignee: Associates of Cape Cod, Inc.Inventors: Thomas J. Novitsky, John L. Sloyer, Jr., Elias R. Elias, Alan Shinn, Chiko Fan
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Patent number: D457081Type: GrantFiled: July 25, 2001Date of Patent: May 14, 2002Assignee: Associates of Cape Cod, Inc.Inventors: Thomas J. Novitsky, John L. Sloyer, Jr., Elias R. Elias, Alan Shinn, Chiko Fan