Abstract: Disclosed is a method for the manipulation for an identification purpose of a microcarrier comprising the steps of: (a) an identification purpose step of the microcarrier; and (b) a positioning and orientation step prior to or during the identification purpose step. Also disclosed is an apparatus for the manipulation for identification purposes of a microcarrier comprising means for identification purposes such as a microscope or labelling means such as a high spatial resolution light source, and means for the positioning and orientation of the microcarriers.

Abstract: Encoded microcarriers, and more specifically microcarriers having codes written on them. Methods for writing the codes on the microcarriers, methods of reading the codes, and methods of using the encoded microcarriers. A preferred method of encoding the microcarriers involves exposing microcarriers containing a bleachable substance to a high spatial resolution light source to bleach the codes on the microcarriers. The encoded microcarriers may be used, for example, as support materials in chemical and biological assays and syntheses.

Abstract: An apparatus for irradiating a liquid sample with acoustic energy to generate cavitation in the liquid sample. The apparatus includes a source for emitting high intensity ultrasound waves and is adapted to receive a cartridge having a chamber containing the liquid sample, wherein the high intensity ultrasound waves are focused on a focus position at or near a nucleation element extending at least partially into the chamber.

Abstract: The present invention relates to a cartridge for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences. The cartridge comprises a first component and a second component being connectable to each other, the first component comprising at least a first fluid opening and a first sealing surface and the second component comprising at least a second fluid opening and a second sealing surface. Upon connection of the first and second component the first and second fluid opening are placeable in fluid communication and the first and second sealing surfaces are placeable against each other to seal the fluid communication between the first and second fluid opening. The invention is characterized in that the cartridge comprises biasing means for biasing the second sealing surface in the direction of the first sealing surface.

Abstract: An apparatus for irradiating a liquid sample with acoustic energy to generate cavitation in the liquid sample is provided. The apparatus includes a source and is adapted to receive a cartridge in such a way, that the apparatus focuses the HIFU waves emitted from the source onto a liquid air interface that is present within the cartridge. This focusing is performed when the cartridge is inserted into a receiving section of the apparatus.

Abstract: A method for irradiating a sample with focused acoustic energy is provided. The generated acoustic energy is transmitted from the source to the sample via a completely dry propagation path. At least two different focal regions are generated with the source for generating acoustic energy.

Abstract: A device for receiving a sample carrier is provided. The device includes an opening for receiving part of the sample carrier and a cutter for removing a part of the sample carrier. The cutter is coupled to a lid, which is movable to allow the cutter to make an incision in the sample carrier and, at the same time, to close at least part of the opening left open after receipt of the sample carrier. The disclosure further relates to a system comprising such a device and a method for operating such a device.

Abstract: A device for treating a sample with focused acoustic energy is provided. The device transmits the generated acoustic energy from the source to the sample via a complete dry propagation path. A cartridge containing the sample is inserted into an instrument, wherein the insertion forms a dry propagation path.

Abstract: A device for receiving a sample carrier is provided. The device includes an opening for receiving part of the sample carrier and a cutter for removing a part of the sample carrier. The cutter is coupled to a lid, which is movable to allow the cutter to make an incision in the sample carrier and, at the same time, to close at least part of the opening left open after receipt of the sample carrier. The disclosure further relates to a system comprising such a device and a method for operating such a device.

Abstract: Methods and devices for the selective lysis of cells in a sample comprising micro-organisms such as bacteria are provided. The selective lysis is obtained by incubating the sample in a non-ionic detergent under alkaline conditions.

Abstract: An optical detection system for monitoring real-time PCR reactions in a plurality of sample chambers with a plurality of optical units is provided. Due to a relative movement, the optical units are relative to the sample chambers, color multiplexing and space multiplexing are combined for optically detecting pathogens in a sample during the process of the PCR and delivering a quantitative result.

Abstract: Silicon microcarriers suitable for fluorescent assays as a well as a method of producing such microcarriers are provided. The method includes the steps of providing a SOI wafer having a bottom layer of monocristalline silicone, an insulator layer and a bottom layer of monocristalline silicon, delineating microparticles, etching away the insulator layer and then depositing an oxide layer on the wafer still holding the microparticles before finally lifting-off the microparticles.

Abstract: A manifold system is provided that includes a manifold housing and a manifold core. The manifold housing is configured to receive the manifold core. The manifold housing includes an oblique inner surface and at least one fluidic channel. The fluidic channel ends with one of its ends at the oblique inner surface. The manifold housing together with the at least one fluidic channel has no undercut.

Abstract: A device for receiving a sample carrier is provided. The device includes an opening for receiving part of the sample carrier and a cutter for removing a part of the sample carrier. The cutter is coupled to a lid, which is movable to allow the cutter to make an incision in the sample carrier and, at the same time, to close at least part of the opening left open after receipt of the sample carrier. The disclosure further relates to a system comprising such a device and a method for operating such a device.

Abstract: A microfluidic cartridge for placement onto a parallel pneumatic interface plate of a pneumatic instrument is provided. The cartridge includes a three dimensional fluid channel, in which a fluid is to be transported, a flexible membrane that spans a plane and is part of an outer surface of the cartridge. The three dimensional fluid channel is spatially defined in three dimensions by internal walls of the cartridge and by the flexible membrane. The flexible membrane is in a ground state, when no pressure or vacuum is applied to the flexible membrane. However, the flexible membrane is pneumatically deflectable from the ground state perpendicular to the plane of the flexible membrane in two directions when the cartridge is placed onto the parallel pneumatic interface plate.

Abstract: A device for receiving a sample carrier is provided. The device includes an opening for receiving part of the sample carrier and a cutter for removing a part of the sample carrier. The cutter is coupled to a lid, which is movable to allow the cutter to make an incision in the sample carrier and, at the same time, to close at least part of the opening left open after receipt of the sample carrier. The disclosure further relates to a system comprising such a device and a method for operating such a device.

Abstract: An optical detection system for monitoring real-time PCR reactions in a plurality of sample chambers with a plurality of optical units is provided. Due to a relative movement, the optical units are relative to the sample chambers, color multiplexing and space multiplexing are combined for optically detecting pathogens in a sample during the process of the PCR and delivering a quantitative result.